Interpretive criteria for mupirocin susceptibility testing of Staphylococcus spp. using CLSI guidelines.

Hdl Handle:
http://hdl.handle.net/10147/209283
Title:
Interpretive criteria for mupirocin susceptibility testing of Staphylococcus spp. using CLSI guidelines.
Authors:
Creagh, S; Lucey, B
Affiliation:
Department of Medical Microbiology, Cork University Hospital, Wilton, Cork,, Ireland.
Citation:
Br J Biomed Sci. 2007;64(1):1-5.
Journal:
British journal of biomedical science
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209283
PubMed ID:
17444410
Abstract:
Mupirocin is an antimicrobial agent commonly used to treat staphylococcal infection or to eliminate persistent carriage. To date, interpretive criteria have not been established to define susceptibility or resistance when performing mupirocin susceptibility testing. In this evaluation, using CLSI guidelines, a total of 502 staphylococci comprising 219 methicillin-sensitive Staphylococcus aureus, 222 methicillin-resistant S. aureus and 61 coagulase-negative staphylococci are tested by broth microdilution, disc diffusion and E-test. Disc diffusion using 5 microg mupirocin discs was found to be a reliable method to distinguish susceptible and resistant strains. Minimum inhibitory concentration (MIC) determination was required to differentiate low-level and high-level resistance to mupirocin. E-test was found to be an accurate alternative to broth microdilution for the routine determination of MIC values of staphylococci to mupirocin. Broth microdilution and disc-diffusion results were plotted on a scattergram, and error rates were calculated. No errors were found using susceptibility criteria of < 4 microg/mL (MIC) and > 19 mm (zone diameter).
Language:
eng
MeSH:
Anti-Bacterial Agents/*pharmacology; *Drug Resistance, Bacterial; Humans; Ireland; Microbial Sensitivity Tests/*methods; Multicenter Studies as Topic; Mupirocin/*pharmacology; Staphylococcal Infections/*drug therapy; Staphylococcus aureus/*drug effects/growth & development
ISSN:
0967-4845 (Print); 0967-4845 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorCreagh, Sen_GB
dc.contributor.authorLucey, Ben_GB
dc.date.accessioned2012-02-03T15:17:09Z-
dc.date.available2012-02-03T15:17:09Z-
dc.date.issued2012-02-03T15:17:09Z-
dc.identifier.citationBr J Biomed Sci. 2007;64(1):1-5.en_GB
dc.identifier.issn0967-4845 (Print)en_GB
dc.identifier.issn0967-4845 (Linking)en_GB
dc.identifier.pmid17444410en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209283-
dc.description.abstractMupirocin is an antimicrobial agent commonly used to treat staphylococcal infection or to eliminate persistent carriage. To date, interpretive criteria have not been established to define susceptibility or resistance when performing mupirocin susceptibility testing. In this evaluation, using CLSI guidelines, a total of 502 staphylococci comprising 219 methicillin-sensitive Staphylococcus aureus, 222 methicillin-resistant S. aureus and 61 coagulase-negative staphylococci are tested by broth microdilution, disc diffusion and E-test. Disc diffusion using 5 microg mupirocin discs was found to be a reliable method to distinguish susceptible and resistant strains. Minimum inhibitory concentration (MIC) determination was required to differentiate low-level and high-level resistance to mupirocin. E-test was found to be an accurate alternative to broth microdilution for the routine determination of MIC values of staphylococci to mupirocin. Broth microdilution and disc-diffusion results were plotted on a scattergram, and error rates were calculated. No errors were found using susceptibility criteria of < 4 microg/mL (MIC) and > 19 mm (zone diameter).en_GB
dc.language.isoengen_GB
dc.subject.meshAnti-Bacterial Agents/*pharmacologyen_GB
dc.subject.mesh*Drug Resistance, Bacterialen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIrelanden_GB
dc.subject.meshMicrobial Sensitivity Tests/*methodsen_GB
dc.subject.meshMulticenter Studies as Topicen_GB
dc.subject.meshMupirocin/*pharmacologyen_GB
dc.subject.meshStaphylococcal Infections/*drug therapyen_GB
dc.subject.meshStaphylococcus aureus/*drug effects/growth & developmenten_GB
dc.titleInterpretive criteria for mupirocin susceptibility testing of Staphylococcus spp. using CLSI guidelines.en_GB
dc.contributor.departmentDepartment of Medical Microbiology, Cork University Hospital, Wilton, Cork,, Ireland.en_GB
dc.identifier.journalBritish journal of biomedical scienceen_GB
dc.description.provinceMunster-

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