Sulphomucin expression in ileal pouches: emerging differences between ulcerative colitis and familial adenomatous polyposis pouches.

Hdl Handle:
http://hdl.handle.net/10147/209141
Title:
Sulphomucin expression in ileal pouches: emerging differences between ulcerative colitis and familial adenomatous polyposis pouches.
Authors:
Bambury, Niamh; Coffey, J Calvin; Burke, John; Redmond, H Paul; Kirwan, William O
Affiliation:
Department of Surgery, University College Cork and Cork University Hospital,, National University of Ireland, Dublin, Ireland.
Citation:
Dis Colon Rectum. 2008 May;51(5):561-7. Epub 2008 Feb 26.
Journal:
Diseases of the colon and rectum
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209141
DOI:
10.1007/s10350-008-9200-0
PubMed ID:
18299930
Abstract:
PURPOSE: We characterized the expression of sialomucin and sulphomucin in pouches fashioned for familial adenomatous polyposis and ulcerative colitis. We correlated sulphomucin expression with bacterial colonization and mucosal inflammation. METHODS: Ethical approval and informed consent were obtained. Mucosal biopsies from 9 patients with familial adenomatous polyposis and 12 with ulcerative colitis were obtained. Sulphomucin levels were assessed by using the high iron-diamine stain. Mucous gel layer composition was correlated with villous height, crypt depth, and total mucosal thickness. Mucous gel layer composition was correlated with acute and chronic inflammatory infiltrates. Colonization by a panel of seven bacterial species (including sulphate reducing bacteria) was established and correlated with sulphomucin levels. RESULTS: High-iron-diamine positivity (i.e., sulphomucin expression) was greater in ulcerative colitis pouch mucous gel (2.083 +/- 0.5 vs. 0.556 +/- 0.4, P = 0.003). Sulphomucin expression correlated with reduced crypt depth, villous height, and total mucosal thickness. In the ulcerative colitis group, chronic inflammatory infiltrate scores were significantly greater for high-iron-diamine-positive patients. Colonization by sulphate reducing bacteria was increased in high-iron-diamine-positive patients. CONCLUSIONS: Sulphomucin expression is increased in the mucous gel layer of the ulcerative colitis pouch compared with that of the familial adenomatous polyposis pouch. Sulphomucin expression is associated with colonization by sulphate-reducing bacteria and increased chronic inflammation.
Language:
eng
MeSH:
Adenomatous Polyposis Coli/*metabolism/surgery; Biopsy; Colitis, Ulcerative/*metabolism/surgery; Colonic Pouches/*microbiology; Female; Humans; Male; Mucins/*metabolism; Statistics, Nonparametric
ISSN:
1530-0358 (Electronic); 0012-3706 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorBambury, Niamhen_GB
dc.contributor.authorCoffey, J Calvinen_GB
dc.contributor.authorBurke, Johnen_GB
dc.contributor.authorRedmond, H Paulen_GB
dc.contributor.authorKirwan, William Oen_GB
dc.date.accessioned2012-02-03T15:13:20Z-
dc.date.available2012-02-03T15:13:20Z-
dc.date.issued2012-02-03T15:13:20Z-
dc.identifier.citationDis Colon Rectum. 2008 May;51(5):561-7. Epub 2008 Feb 26.en_GB
dc.identifier.issn1530-0358 (Electronic)en_GB
dc.identifier.issn0012-3706 (Linking)en_GB
dc.identifier.pmid18299930en_GB
dc.identifier.doi10.1007/s10350-008-9200-0en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209141-
dc.description.abstractPURPOSE: We characterized the expression of sialomucin and sulphomucin in pouches fashioned for familial adenomatous polyposis and ulcerative colitis. We correlated sulphomucin expression with bacterial colonization and mucosal inflammation. METHODS: Ethical approval and informed consent were obtained. Mucosal biopsies from 9 patients with familial adenomatous polyposis and 12 with ulcerative colitis were obtained. Sulphomucin levels were assessed by using the high iron-diamine stain. Mucous gel layer composition was correlated with villous height, crypt depth, and total mucosal thickness. Mucous gel layer composition was correlated with acute and chronic inflammatory infiltrates. Colonization by a panel of seven bacterial species (including sulphate reducing bacteria) was established and correlated with sulphomucin levels. RESULTS: High-iron-diamine positivity (i.e., sulphomucin expression) was greater in ulcerative colitis pouch mucous gel (2.083 +/- 0.5 vs. 0.556 +/- 0.4, P = 0.003). Sulphomucin expression correlated with reduced crypt depth, villous height, and total mucosal thickness. In the ulcerative colitis group, chronic inflammatory infiltrate scores were significantly greater for high-iron-diamine-positive patients. Colonization by sulphate reducing bacteria was increased in high-iron-diamine-positive patients. CONCLUSIONS: Sulphomucin expression is increased in the mucous gel layer of the ulcerative colitis pouch compared with that of the familial adenomatous polyposis pouch. Sulphomucin expression is associated with colonization by sulphate-reducing bacteria and increased chronic inflammation.en_GB
dc.language.isoengen_GB
dc.subject.meshAdenomatous Polyposis Coli/*metabolism/surgeryen_GB
dc.subject.meshBiopsyen_GB
dc.subject.meshColitis, Ulcerative/*metabolism/surgeryen_GB
dc.subject.meshColonic Pouches/*microbiologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMucins/*metabolismen_GB
dc.subject.meshStatistics, Nonparametricen_GB
dc.titleSulphomucin expression in ileal pouches: emerging differences between ulcerative colitis and familial adenomatous polyposis pouches.en_GB
dc.contributor.departmentDepartment of Surgery, University College Cork and Cork University Hospital,, National University of Ireland, Dublin, Ireland.en_GB
dc.identifier.journalDiseases of the colon and rectumen_GB
dc.description.provinceMunster-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.