Neutrophil-induced transmigration of tumour cells treated with tumour-conditioned medium is facilitated by granulocyte-macrophage colony-stimulating factor.

Hdl Handle:
http://hdl.handle.net/10147/209116
Title:
Neutrophil-induced transmigration of tumour cells treated with tumour-conditioned medium is facilitated by granulocyte-macrophage colony-stimulating factor.
Authors:
Wu, Q D; Wang, J H; Bouchier-Hayes, D; Redmond, H P
Affiliation:
Department of Surgery, Cork University Hospital, University College Cork,, Ireland.
Citation:
Eur J Surg. 2000 May;166(5):361-6.
Journal:
The European journal of surgery = Acta chirurgica
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209116
DOI:
10.1080/110241500750008899
PubMed ID:
10881945
Abstract:
OBJECTIVE: To investigate the effect of different cytokines that are present in tumour-conditioned medium on human neutrophil (PMN)-induced tumour cell transmigration. DESIGN: Laboratory study. SETTING: University hospital, Ireland. MATERIAL: Isolated human PMN and cultured human breast tumour cell line, MDA-MB-231. Interventions: Human PMN treated with either tumour-conditioned medium or different media neutralised with monoclonal antibodies (MoAb), and MDA-MB-231 cells were plated on macrovascular and microvascular endothelial monolayers in collagen-coated transwells to assess migration of tumour cells. MAIN OUTCOME MEASURES: Cytokines present in tumour-conditioned medium, PMN cytocidal function and receptor expression, and tumour cell transmigration. RESULTS: tumour-conditioned medium contained high concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and interleukin 8 (IL-8), but not granulocyte colony-stimulating factor (G-CSF) and interleukin 3 (IL-3). Anti-GM-CSF MoAb significantly reduced PMN-induced transmigration of tumour cells treated with tumour-conditioned medium (p < 0.05), whereas anti-VEGF and anti-IL-8 MoAbs did not affect their migration. In addition, anti-GM-CSF MoAb, but not anti-VEGF or anti-IL-8 MoAb, reduced PMN CD11b and CD18 overexpression induced by tumour-conditioned medium (p < 0.05). CONCLUSION: These results indicate that the GM-CSF that is present in tumour-conditioned medium may be involved, at least in part, in alterations in PMN function mediated by the medium and subsequently PMN-induced transmigration of tumour cells.
Language:
eng
MeSH:
Breast Neoplasms/*pathology/physiopathology; Cell Adhesion Molecules; Cell Movement; Culture Media; Cytokines/physiology; Endothelium, Vascular; Female; Granulocyte-Macrophage Colony-Stimulating Factor/*physiology; Humans; Interleukin-3/physiology; Interleukin-8/physiology; Neutrophils/*physiology; Tumor Cells, Cultured/*physiology
ISSN:
1102-4151 (Print); 1102-4151 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorWu, Q Den_GB
dc.contributor.authorWang, J Hen_GB
dc.contributor.authorBouchier-Hayes, Den_GB
dc.contributor.authorRedmond, H Pen_GB
dc.date.accessioned2012-02-03T15:12:41Z-
dc.date.available2012-02-03T15:12:41Z-
dc.date.issued2012-02-03T15:12:41Z-
dc.identifier.citationEur J Surg. 2000 May;166(5):361-6.en_GB
dc.identifier.issn1102-4151 (Print)en_GB
dc.identifier.issn1102-4151 (Linking)en_GB
dc.identifier.pmid10881945en_GB
dc.identifier.doi10.1080/110241500750008899en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209116-
dc.description.abstractOBJECTIVE: To investigate the effect of different cytokines that are present in tumour-conditioned medium on human neutrophil (PMN)-induced tumour cell transmigration. DESIGN: Laboratory study. SETTING: University hospital, Ireland. MATERIAL: Isolated human PMN and cultured human breast tumour cell line, MDA-MB-231. Interventions: Human PMN treated with either tumour-conditioned medium or different media neutralised with monoclonal antibodies (MoAb), and MDA-MB-231 cells were plated on macrovascular and microvascular endothelial monolayers in collagen-coated transwells to assess migration of tumour cells. MAIN OUTCOME MEASURES: Cytokines present in tumour-conditioned medium, PMN cytocidal function and receptor expression, and tumour cell transmigration. RESULTS: tumour-conditioned medium contained high concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and interleukin 8 (IL-8), but not granulocyte colony-stimulating factor (G-CSF) and interleukin 3 (IL-3). Anti-GM-CSF MoAb significantly reduced PMN-induced transmigration of tumour cells treated with tumour-conditioned medium (p < 0.05), whereas anti-VEGF and anti-IL-8 MoAbs did not affect their migration. In addition, anti-GM-CSF MoAb, but not anti-VEGF or anti-IL-8 MoAb, reduced PMN CD11b and CD18 overexpression induced by tumour-conditioned medium (p < 0.05). CONCLUSION: These results indicate that the GM-CSF that is present in tumour-conditioned medium may be involved, at least in part, in alterations in PMN function mediated by the medium and subsequently PMN-induced transmigration of tumour cells.en_GB
dc.language.isoengen_GB
dc.subject.meshBreast Neoplasms/*pathology/physiopathologyen_GB
dc.subject.meshCell Adhesion Moleculesen_GB
dc.subject.meshCell Movementen_GB
dc.subject.meshCulture Mediaen_GB
dc.subject.meshCytokines/physiologyen_GB
dc.subject.meshEndothelium, Vascularen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor/*physiologyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshInterleukin-3/physiologyen_GB
dc.subject.meshInterleukin-8/physiologyen_GB
dc.subject.meshNeutrophils/*physiologyen_GB
dc.subject.meshTumor Cells, Cultured/*physiologyen_GB
dc.titleNeutrophil-induced transmigration of tumour cells treated with tumour-conditioned medium is facilitated by granulocyte-macrophage colony-stimulating factor.en_GB
dc.contributor.departmentDepartment of Surgery, Cork University Hospital, University College Cork,, Ireland.en_GB
dc.identifier.journalThe European journal of surgery = Acta chirurgicaen_GB
dc.description.provinceMunster-
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