The ligase chain reaction as a primary screening tool for the detection of culture positive tuberculosis.

Hdl Handle:
http://hdl.handle.net/10147/209103
Title:
The ligase chain reaction as a primary screening tool for the detection of culture positive tuberculosis.
Authors:
O'Connor, T M; Sheehan, S; Cryan, B; Brennan, N; Bredin, C P
Affiliation:
Department of Respiratory Medicine, Cork University Hospital, Cork, Ireland., terryoconnor@eircom.net
Citation:
Thorax. 2000 Nov;55(11):955-7.
Journal:
Thorax
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209103
PubMed ID:
11050266
Abstract:
BACKGROUND: The ligase chain reaction Mycobacterium tuberculosis assay uses ligase chain reaction technology to detect tuberculous DNA sequences in clinical specimens. A study was undertaken to determine its sensitivity and specificity as a primary screening tool for the detection of culture positive tuberculosis. METHODS: The study was conducted on 2420 clinical specimens (sputum, bronchoalveolar lavage fluid, pleural fluid, urine) submitted for primary screening for Mycobacterium tuberculosis to a regional medical microbiology laboratory. Specimens were tested in parallel with smear, ligase chain reaction, and culture. RESULTS: Thirty nine patients had specimens testing positive by the ligase chain reaction assay. Thirty two patients had newly diagnosed tuberculosis, one had a tuberculosis relapse, three had tuberculosis (on antituberculous therapy when tested), and three had healed tuberculosis. In the newly diagnosed group specimens were smear positive in 21 cases (66%), ligase chain reaction positive in 30 cases (94%), and culture positive in 32 cases (100%). Using a positive culture to diagnose active tuberculosis, the ligase chain reaction assay had a sensitivity of 93.9%, a specificity of 99.8%, a positive predictive value of 83.8%, and a negative predictive value of 99.9%. CONCLUSIONS: This study is the largest clinical trial to date to report the efficacy of the ligase chain reaction as a primary screening tool to detect Mycobacterium tuberculosis infection. The authors conclude that ligase chain reaction is a useful primary screening test for tuberculosis, offering speed and discrimination in the early stages of diagnosis and complementing traditional smear and culture techniques.
Language:
eng
MeSH:
Adolescent; Adult; Aged; Aged, 80 and over; Body Fluids/chemistry; Bronchoalveolar Lavage Fluid/chemistry; DNA, Bacterial/analysis; Female; Humans; Ligase Chain Reaction/*standards; Male; Mass Screening/methods; Middle Aged; Mycobacterium tuberculosis/*isolation & purification; Sensitivity and Specificity; Sputum/chemistry; Tuberculosis/*diagnosis/urine
ISSN:
0040-6376 (Print); 0040-6376 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Connor, T Men_GB
dc.contributor.authorSheehan, Sen_GB
dc.contributor.authorCryan, Ben_GB
dc.contributor.authorBrennan, Nen_GB
dc.contributor.authorBredin, C Pen_GB
dc.date.accessioned2012-02-03T15:12:21Z-
dc.date.available2012-02-03T15:12:21Z-
dc.date.issued2012-02-03T15:12:21Z-
dc.identifier.citationThorax. 2000 Nov;55(11):955-7.en_GB
dc.identifier.issn0040-6376 (Print)en_GB
dc.identifier.issn0040-6376 (Linking)en_GB
dc.identifier.pmid11050266en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209103-
dc.description.abstractBACKGROUND: The ligase chain reaction Mycobacterium tuberculosis assay uses ligase chain reaction technology to detect tuberculous DNA sequences in clinical specimens. A study was undertaken to determine its sensitivity and specificity as a primary screening tool for the detection of culture positive tuberculosis. METHODS: The study was conducted on 2420 clinical specimens (sputum, bronchoalveolar lavage fluid, pleural fluid, urine) submitted for primary screening for Mycobacterium tuberculosis to a regional medical microbiology laboratory. Specimens were tested in parallel with smear, ligase chain reaction, and culture. RESULTS: Thirty nine patients had specimens testing positive by the ligase chain reaction assay. Thirty two patients had newly diagnosed tuberculosis, one had a tuberculosis relapse, three had tuberculosis (on antituberculous therapy when tested), and three had healed tuberculosis. In the newly diagnosed group specimens were smear positive in 21 cases (66%), ligase chain reaction positive in 30 cases (94%), and culture positive in 32 cases (100%). Using a positive culture to diagnose active tuberculosis, the ligase chain reaction assay had a sensitivity of 93.9%, a specificity of 99.8%, a positive predictive value of 83.8%, and a negative predictive value of 99.9%. CONCLUSIONS: This study is the largest clinical trial to date to report the efficacy of the ligase chain reaction as a primary screening tool to detect Mycobacterium tuberculosis infection. The authors conclude that ligase chain reaction is a useful primary screening test for tuberculosis, offering speed and discrimination in the early stages of diagnosis and complementing traditional smear and culture techniques.en_GB
dc.language.isoengen_GB
dc.subject.meshAdolescenten_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshAged, 80 and overen_GB
dc.subject.meshBody Fluids/chemistryen_GB
dc.subject.meshBronchoalveolar Lavage Fluid/chemistryen_GB
dc.subject.meshDNA, Bacterial/analysisen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLigase Chain Reaction/*standardsen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMass Screening/methodsen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshMycobacterium tuberculosis/*isolation & purificationen_GB
dc.subject.meshSensitivity and Specificityen_GB
dc.subject.meshSputum/chemistryen_GB
dc.subject.meshTuberculosis/*diagnosis/urineen_GB
dc.titleThe ligase chain reaction as a primary screening tool for the detection of culture positive tuberculosis.en_GB
dc.contributor.departmentDepartment of Respiratory Medicine, Cork University Hospital, Cork, Ireland., terryoconnor@eircom.neten_GB
dc.identifier.journalThoraxen_GB
dc.description.provinceMunster-

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