Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.

Hdl Handle:
http://hdl.handle.net/10147/209098
Title:
Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.
Authors:
Levis, J; Kenny-Walsh, E; O'Sullivan, K; Horgan, M; Whelton, M; Shanahan, F; Fanning, L
Affiliation:
Hepatitis C Unit, Department of Medicine, Clinical Sciences Building, Cork, University Hospital, University College, Cork, Ireland.
Citation:
J Clin Virol. 2001 Feb;20(3):163-71.
Journal:
Journal of clinical virology : the official publication of the Pan American, Society for Clinical Virology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209098
PubMed ID:
11166666
Abstract:
BACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient's first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.
Language:
eng
MeSH:
DNA, Viral/analysis; Enzyme-Linked Immunosorbent Assay; Female; Genotype; Hepacivirus/*genetics; Hepatitis C/*virology; Humans; Male; Prospective Studies; RNA, Viral/blood; Reagent Kits, Diagnostic; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; Viral Load; Viremia/virology
ISSN:
1386-6532 (Print); 1386-6532 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorLevis, Jen_GB
dc.contributor.authorKenny-Walsh, Een_GB
dc.contributor.authorO'Sullivan, Ken_GB
dc.contributor.authorHorgan, Men_GB
dc.contributor.authorWhelton, Men_GB
dc.contributor.authorShanahan, Fen_GB
dc.contributor.authorFanning, Len_GB
dc.date.accessioned2012-02-03T15:12:13Z-
dc.date.available2012-02-03T15:12:13Z-
dc.date.issued2012-02-03T15:12:13Z-
dc.identifier.citationJ Clin Virol. 2001 Feb;20(3):163-71.en_GB
dc.identifier.issn1386-6532 (Print)en_GB
dc.identifier.issn1386-6532 (Linking)en_GB
dc.identifier.pmid11166666en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209098-
dc.description.abstractBACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient's first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.en_GB
dc.language.isoengen_GB
dc.subject.meshDNA, Viral/analysisen_GB
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshGenotypeen_GB
dc.subject.meshHepacivirus/*geneticsen_GB
dc.subject.meshHepatitis C/*virologyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMaleen_GB
dc.subject.meshProspective Studiesen_GB
dc.subject.meshRNA, Viral/blooden_GB
dc.subject.meshReagent Kits, Diagnosticen_GB
dc.subject.meshRetrospective Studiesen_GB
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_GB
dc.subject.meshViral Loaden_GB
dc.subject.meshViremia/virologyen_GB
dc.titleStrategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.en_GB
dc.contributor.departmentHepatitis C Unit, Department of Medicine, Clinical Sciences Building, Cork, University Hospital, University College, Cork, Ireland.en_GB
dc.identifier.journalJournal of clinical virology : the official publication of the Pan American, Society for Clinical Virologyen_GB
dc.description.provinceMunster-
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