Sodium hyaluronate enhances colorectal tumour cell metastatic potential in vitro and in vivo.

Hdl Handle:
http://hdl.handle.net/10147/209095
Title:
Sodium hyaluronate enhances colorectal tumour cell metastatic potential in vitro and in vivo.
Authors:
Tan, B; Wang, J H; Wu, Q D; Kirwan, W O; Redmond, H P
Affiliation:
Department of Academic Surgery, Cork University Hospital, University College, Cork, Cork, Ireland.
Citation:
Br J Surg. 2001 Feb;88(2):246-50.
Journal:
The British journal of surgery
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209095
DOI:
10.1046/j.1365-2168.2001.01664.x
PubMed ID:
11167875
Abstract:
BACKGROUND: Sodium hyaluronate has been used intraperitoneally to prevent postoperative adhesions. However, the effect of sodium hyaluronate on tumour growth and metastasis in vitro and in vivo is still unknown. METHODS: Human colorectal tumour cell lines SW480, SW620 and SW707 were treated with sodium hyaluronate (10-500 microg/ml) and carboxymethylcellulose (0.125-1 per cent), and tumour cell proliferation and motility were determined in vitro. For the in vivo experiments male BD IX rats were randomized to a sodium hyaluronate group (n = 11; intraperitoneal administration of 0.5 x 10(6) DHD/K12 tumour cells and 5 ml 0.4 per cent sodium hyaluronate) or a phosphate-buffered saline group (n = 11; 0.5 x 10(6) DHD/K12 tumour cells and 5 ml phosphate-buffered saline intraperitoneally). Four weeks later the intraperitoneal tumour load was visualized directly. RESULTS: In vitro sodium hyaluronate increased tumour cell proliferation and motility significantly. Sodium hyaluronate-induced tumour cell motility appeared to be CD44 receptor dependent, whereas sodium hyaluronate-induced tumour cell proliferation was CD44 receptor independent. In vivo there was a significantly higher total tumour nodule count in the peritoneal cavity of the sodium hyaluronate-treated group compared with the control (P = 0.016). CONCLUSION: Sodium hyaluronate enhances tumour metastatic potential in vitro and in vivo, which suggests that use of sodium hyaluronate to prevent adhesions in colorectal cancer surgery may also potentiate intraperitoneal tumour growth. Presented to the Patey Prize Session of the Surgical Research Society and the annual scientific meeting of the Association of Surgeons of Great Britain and Ireland, Brighton, UK, 4-7 May 1999
Language:
eng
MeSH:
Animals; Antigens, CD44/metabolism; Cell Division/drug effects; Cell Movement; Colorectal Neoplasms/*pathology; Humans; Hyaluronic Acid/*adverse effects; Male; Neoplasm Metastasis/*pathology; Peritoneal Neoplasms/pathology; Rats; Tumor Cells, Cultured/drug effects
ISSN:
0007-1323 (Print); 0007-1323 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorTan, Ben_GB
dc.contributor.authorWang, J Hen_GB
dc.contributor.authorWu, Q Den_GB
dc.contributor.authorKirwan, W Oen_GB
dc.contributor.authorRedmond, H Pen_GB
dc.date.accessioned2012-02-03T15:12:08Z-
dc.date.available2012-02-03T15:12:08Z-
dc.date.issued2012-02-03T15:12:08Z-
dc.identifier.citationBr J Surg. 2001 Feb;88(2):246-50.en_GB
dc.identifier.issn0007-1323 (Print)en_GB
dc.identifier.issn0007-1323 (Linking)en_GB
dc.identifier.pmid11167875en_GB
dc.identifier.doi10.1046/j.1365-2168.2001.01664.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/209095-
dc.description.abstractBACKGROUND: Sodium hyaluronate has been used intraperitoneally to prevent postoperative adhesions. However, the effect of sodium hyaluronate on tumour growth and metastasis in vitro and in vivo is still unknown. METHODS: Human colorectal tumour cell lines SW480, SW620 and SW707 were treated with sodium hyaluronate (10-500 microg/ml) and carboxymethylcellulose (0.125-1 per cent), and tumour cell proliferation and motility were determined in vitro. For the in vivo experiments male BD IX rats were randomized to a sodium hyaluronate group (n = 11; intraperitoneal administration of 0.5 x 10(6) DHD/K12 tumour cells and 5 ml 0.4 per cent sodium hyaluronate) or a phosphate-buffered saline group (n = 11; 0.5 x 10(6) DHD/K12 tumour cells and 5 ml phosphate-buffered saline intraperitoneally). Four weeks later the intraperitoneal tumour load was visualized directly. RESULTS: In vitro sodium hyaluronate increased tumour cell proliferation and motility significantly. Sodium hyaluronate-induced tumour cell motility appeared to be CD44 receptor dependent, whereas sodium hyaluronate-induced tumour cell proliferation was CD44 receptor independent. In vivo there was a significantly higher total tumour nodule count in the peritoneal cavity of the sodium hyaluronate-treated group compared with the control (P = 0.016). CONCLUSION: Sodium hyaluronate enhances tumour metastatic potential in vitro and in vivo, which suggests that use of sodium hyaluronate to prevent adhesions in colorectal cancer surgery may also potentiate intraperitoneal tumour growth. Presented to the Patey Prize Session of the Surgical Research Society and the annual scientific meeting of the Association of Surgeons of Great Britain and Ireland, Brighton, UK, 4-7 May 1999en_GB
dc.language.isoengen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshAntigens, CD44/metabolismen_GB
dc.subject.meshCell Division/drug effectsen_GB
dc.subject.meshCell Movementen_GB
dc.subject.meshColorectal Neoplasms/*pathologyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshHyaluronic Acid/*adverse effectsen_GB
dc.subject.meshMaleen_GB
dc.subject.meshNeoplasm Metastasis/*pathologyen_GB
dc.subject.meshPeritoneal Neoplasms/pathologyen_GB
dc.subject.meshRatsen_GB
dc.subject.meshTumor Cells, Cultured/drug effectsen_GB
dc.titleSodium hyaluronate enhances colorectal tumour cell metastatic potential in vitro and in vivo.en_GB
dc.contributor.departmentDepartment of Academic Surgery, Cork University Hospital, University College, Cork, Cork, Ireland.en_GB
dc.identifier.journalThe British journal of surgeryen_GB
dc.description.provinceMunster-

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