The effect of the anaesthetic agent isoflurane on the rate of neutrophil apoptosis in vitro.

Hdl Handle:
http://hdl.handle.net/10147/209073
Title:
The effect of the anaesthetic agent isoflurane on the rate of neutrophil apoptosis in vitro.
Authors:
Tyther, R; Fanning, N; Halligan, M; Wang, J; Redmond, H P; Shorten, G
Affiliation:
Department of Anaesthesia and Intensive Care Medicine, Cork University Hospital, , University College Cork, Ireland.
Citation:
Ir J Med Sci. 2001 Jan-Mar;170(1):41-4.
Journal:
Irish journal of medical science
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209073
PubMed ID:
11440412
Abstract:
BACKGROUND: Volatile anaesthetic agents influence neutrophil function, and potentially, the inflammatory response to surgery. AIM: The objective of this study was to determine the effect of isoflurane (1-4%) on human polymorphonuclear neutrophil apoptosis in vitro. METHODS: Venous blood from 12 healthy volunteers was exposed to 0, 1, and 4% isoflurane delivered via a 14G Wallace flexihub internal jugular cannula, at a fresh gas flow of 0.51/min for 5 minutes. Isolated neutrophils were assessed for apoptosis at 1, 12, and 24 hours in culture using dual staining with annexin V-FITC and propidium iodide (Annexin-V FITC assay). Data were analysed using paired, one-tailed Student's t-tests. p<0.05 was considered significant. RESULTS: At 1 hour apoptosis was inhibited in the 1% (5.1 [6.8]%; p=0.017) and 4% (4.8 [4.5]%; p=0.008) isoflurane groups compared to control (11.3 [6.9]%). At 12 and 24 hours, a dose-dependent inhibition of apoptosis was demonstrated, i.e. 4% > 1% > 0%. CONCLUSION: Human neutrophil apoptosis is inhibited in a concentration-dependent manner in vitro by isoflurane in clinical concentrations.
Language:
eng
MeSH:
Adult; Anesthetics, Inhalation/*pharmacology; Apoptosis/*drug effects; Cells, Cultured; Flow Cytometry; Humans; Isoflurane/*pharmacology; Neutrophils/*drug effects; Time Factors
ISSN:
0021-1265 (Print); 0021-1265 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorTyther, Ren_GB
dc.contributor.authorFanning, Nen_GB
dc.contributor.authorHalligan, Men_GB
dc.contributor.authorWang, Jen_GB
dc.contributor.authorRedmond, H Pen_GB
dc.contributor.authorShorten, Gen_GB
dc.date.accessioned2012-02-03T15:11:33Z-
dc.date.available2012-02-03T15:11:33Z-
dc.date.issued2012-02-03T15:11:33Z-
dc.identifier.citationIr J Med Sci. 2001 Jan-Mar;170(1):41-4.en_GB
dc.identifier.issn0021-1265 (Print)en_GB
dc.identifier.issn0021-1265 (Linking)en_GB
dc.identifier.pmid11440412en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209073-
dc.description.abstractBACKGROUND: Volatile anaesthetic agents influence neutrophil function, and potentially, the inflammatory response to surgery. AIM: The objective of this study was to determine the effect of isoflurane (1-4%) on human polymorphonuclear neutrophil apoptosis in vitro. METHODS: Venous blood from 12 healthy volunteers was exposed to 0, 1, and 4% isoflurane delivered via a 14G Wallace flexihub internal jugular cannula, at a fresh gas flow of 0.51/min for 5 minutes. Isolated neutrophils were assessed for apoptosis at 1, 12, and 24 hours in culture using dual staining with annexin V-FITC and propidium iodide (Annexin-V FITC assay). Data were analysed using paired, one-tailed Student's t-tests. p<0.05 was considered significant. RESULTS: At 1 hour apoptosis was inhibited in the 1% (5.1 [6.8]%; p=0.017) and 4% (4.8 [4.5]%; p=0.008) isoflurane groups compared to control (11.3 [6.9]%). At 12 and 24 hours, a dose-dependent inhibition of apoptosis was demonstrated, i.e. 4% > 1% > 0%. CONCLUSION: Human neutrophil apoptosis is inhibited in a concentration-dependent manner in vitro by isoflurane in clinical concentrations.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAnesthetics, Inhalation/*pharmacologyen_GB
dc.subject.meshApoptosis/*drug effectsen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshFlow Cytometryen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIsoflurane/*pharmacologyen_GB
dc.subject.meshNeutrophils/*drug effectsen_GB
dc.subject.meshTime Factorsen_GB
dc.titleThe effect of the anaesthetic agent isoflurane on the rate of neutrophil apoptosis in vitro.en_GB
dc.contributor.departmentDepartment of Anaesthesia and Intensive Care Medicine, Cork University Hospital, , University College Cork, Ireland.en_GB
dc.identifier.journalIrish journal of medical scienceen_GB
dc.description.provinceMunster-

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