Tissue viral load variability in chronic hepatitis C.

Hdl Handle:
http://hdl.handle.net/10147/209055
Title:
Tissue viral load variability in chronic hepatitis C.
Authors:
Fanning, L; Loane, J; Kenny-Walsh, E; Sheehan, M; Whelton, M; Kirwan, W; Collins, J K; Shanahan, F
Affiliation:
Department of Medicine, Cork University Hospital, National University of Ireland.
Citation:
Am J Gastroenterol. 2001 Dec;96(12):3384-9.
Journal:
The American journal of gastroenterology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209055
DOI:
10.1111/j.1572-0241.2001.05271.x
PubMed ID:
11774953
Abstract:
OBJECTIVE: Liver biopsy is regarded as the gold standard for assessing disease activity in chronic hepatitis C, but sampling error is a potential limitation. Whether sampling variability applies equally to viral load assessment as it does to histology is uncertain. To examine this, we compared viral load between right- and left-lobe biopsy specimens from patients infected with hepatitis C virus (HCV). METHODS: Bilobe biopsies were taken from 16 patients who were serum positive for HCV RNA by reverse transcription-polymerase chain reaction. Genotype was identified by reverse line probe hybridization. There was an absence of competing risk factors for infectious and other liver diseases in this patient group. Histology and hepatic viral load were assessed blindly. None of the patients had received antiviral therapy at the time of study. RESULTS: Detection of HCV in right and left lobes was concordant with serum positivity in all cases. The viral load between lobes was highly correlated (p = 0.0003, r = 0.79). In contrast, the histological activity indices of inflammation and fibrosis/cirrhosis were poorly correlated between lobes (p = 0.038, r = 0.60, and p = 0.098, r = 0.50, respectively). CONCLUSION: Hepatic viral load variability does not suffer from the same degree of heterogeneity of sampling variability as does histology.
Language:
eng
MeSH:
Adult; Aged; Blood/virology; Female; Fibrosis; Hepacivirus/*isolation & purification; Hepatitis C, Chronic/blood/pathology/*virology; Humans; Liver/pathology/*virology; Male; Middle Aged; Viral Load
ISSN:
0002-9270 (Print); 0002-9270 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorFanning, Len_GB
dc.contributor.authorLoane, Jen_GB
dc.contributor.authorKenny-Walsh, Een_GB
dc.contributor.authorSheehan, Men_GB
dc.contributor.authorWhelton, Men_GB
dc.contributor.authorKirwan, Wen_GB
dc.contributor.authorCollins, J Ken_GB
dc.contributor.authorShanahan, Fen_GB
dc.date.accessioned2012-02-03T15:11:03Z-
dc.date.available2012-02-03T15:11:03Z-
dc.date.issued2012-02-03T15:11:03Z-
dc.identifier.citationAm J Gastroenterol. 2001 Dec;96(12):3384-9.en_GB
dc.identifier.issn0002-9270 (Print)en_GB
dc.identifier.issn0002-9270 (Linking)en_GB
dc.identifier.pmid11774953en_GB
dc.identifier.doi10.1111/j.1572-0241.2001.05271.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/209055-
dc.description.abstractOBJECTIVE: Liver biopsy is regarded as the gold standard for assessing disease activity in chronic hepatitis C, but sampling error is a potential limitation. Whether sampling variability applies equally to viral load assessment as it does to histology is uncertain. To examine this, we compared viral load between right- and left-lobe biopsy specimens from patients infected with hepatitis C virus (HCV). METHODS: Bilobe biopsies were taken from 16 patients who were serum positive for HCV RNA by reverse transcription-polymerase chain reaction. Genotype was identified by reverse line probe hybridization. There was an absence of competing risk factors for infectious and other liver diseases in this patient group. Histology and hepatic viral load were assessed blindly. None of the patients had received antiviral therapy at the time of study. RESULTS: Detection of HCV in right and left lobes was concordant with serum positivity in all cases. The viral load between lobes was highly correlated (p = 0.0003, r = 0.79). In contrast, the histological activity indices of inflammation and fibrosis/cirrhosis were poorly correlated between lobes (p = 0.038, r = 0.60, and p = 0.098, r = 0.50, respectively). CONCLUSION: Hepatic viral load variability does not suffer from the same degree of heterogeneity of sampling variability as does histology.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshBlood/virologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshFibrosisen_GB
dc.subject.meshHepacivirus/*isolation & purificationen_GB
dc.subject.meshHepatitis C, Chronic/blood/pathology/*virologyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLiver/pathology/*virologyen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshViral Loaden_GB
dc.titleTissue viral load variability in chronic hepatitis C.en_GB
dc.contributor.departmentDepartment of Medicine, Cork University Hospital, National University of Ireland.en_GB
dc.identifier.journalThe American journal of gastroenterologyen_GB
dc.description.provinceMunster-

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