Sulfate-reducing bacteria colonize pouches formed for ulcerative colitis but not for familial adenomatous polyposis.

Hdl Handle:
http://hdl.handle.net/10147/209038
Title:
Sulfate-reducing bacteria colonize pouches formed for ulcerative colitis but not for familial adenomatous polyposis.
Authors:
Duffy, M; O'Mahony, L; Coffey, J C; Collins, J K; Shanahan, F; Redmond, H P; Kirwan, W O
Affiliation:
Department of Surgery, Cork University Hospital, Ireland.
Citation:
Dis Colon Rectum. 2002 Mar;45(3):384-8.
Journal:
Diseases of the colon and rectum
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209038
PubMed ID:
12068199
Abstract:
PURPOSE: Ileal pouch-anal anastomosis remains the "gold standard" in surgical treatment of ulcerative colitis and familial adenomatous polyposis. Pouchitis occurs mainly in patients with a background of ulcerative colitis, although the reasons for this are unknown. The aim of this study was to characterize differences in pouch bacterial populations between ulcerative colitis and familial adenomatous pouches. METHODS: After ethical approval was obtained, fresh stool samples were collected from patients with ulcerative colitis pouches (n = 10), familial adenomatous polyposis (n = 7) pouches, and ulcerative colitis ileostomies (n = 8). Quantitative measurements of aerobic and anaerobic bacteria were performed. RESULTS: Sulfate-reducing bacteria were isolated from 80 percent (n = 8) of ulcerative colitis pouches. Sulfate-reducing bacteria were absent from familial adenomatous polyposis pouches and also from ulcerative colitis ileostomy effluent. Pouch Lactobacilli, Bifidobacterium, Bacteroides sp, and Clostridium perfringens counts were increased relative to ileostomy counts in patients with ulcerative colitis. Total pouch enterococci and coliform counts were also increased relative to ileostomy levels. There were no significant quantitative or qualitative differences between pouch types when these bacteria were evaluated. CONCLUSIONS: Sulfate-reducing bacteria are exclusive to patients with a background of ulcerative colitis. Not all ulcerative colitis pouches harbor sulfate-reducing bacteria because two ulcerative colitis pouches in this study were free of the latter. They are not present in familial adenomatous polyposis pouches or in ileostomy effluent collected from patients with ulcerative colitis. Total bacterial counts increase in ulcerative colitis pouches after stoma closure. Levels of Lactobacilli, Bifidobacterium, Bacteroides sp, Clostridium perfringens, enterococci, and coliforms were similar in both pouch groups. Because sulfate-reducing bacteria are specific to ulcerative colitis pouches, they may play a role in the pathogenesis of pouchitis.
Language:
eng
MeSH:
Adenomatous Polyposis Coli/*microbiology/physiopathology/*surgery; Adult; Bacteria, Aerobic/*isolation & purification; Bacteria, Anaerobic/*isolation & purification; Colitis, Ulcerative/*microbiology/physiopathology/*surgery; Colony Count, Microbial; Defecation/physiology; Feces/microbiology; Female; Humans; Ileostomy/adverse effects; Male; Pouchitis/*etiology/*microbiology/physiopathology; Proctocolectomy, Restorative/*adverse effects; Sulfates/*isolation & purification
ISSN:
0012-3706 (Print); 0012-3706 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorDuffy, Men_GB
dc.contributor.authorO'Mahony, Len_GB
dc.contributor.authorCoffey, J Cen_GB
dc.contributor.authorCollins, J Ken_GB
dc.contributor.authorShanahan, Fen_GB
dc.contributor.authorRedmond, H Pen_GB
dc.contributor.authorKirwan, W Oen_GB
dc.date.accessioned2012-02-03T15:10:35Z-
dc.date.available2012-02-03T15:10:35Z-
dc.date.issued2012-02-03T15:10:35Z-
dc.identifier.citationDis Colon Rectum. 2002 Mar;45(3):384-8.en_GB
dc.identifier.issn0012-3706 (Print)en_GB
dc.identifier.issn0012-3706 (Linking)en_GB
dc.identifier.pmid12068199en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209038-
dc.description.abstractPURPOSE: Ileal pouch-anal anastomosis remains the "gold standard" in surgical treatment of ulcerative colitis and familial adenomatous polyposis. Pouchitis occurs mainly in patients with a background of ulcerative colitis, although the reasons for this are unknown. The aim of this study was to characterize differences in pouch bacterial populations between ulcerative colitis and familial adenomatous pouches. METHODS: After ethical approval was obtained, fresh stool samples were collected from patients with ulcerative colitis pouches (n = 10), familial adenomatous polyposis (n = 7) pouches, and ulcerative colitis ileostomies (n = 8). Quantitative measurements of aerobic and anaerobic bacteria were performed. RESULTS: Sulfate-reducing bacteria were isolated from 80 percent (n = 8) of ulcerative colitis pouches. Sulfate-reducing bacteria were absent from familial adenomatous polyposis pouches and also from ulcerative colitis ileostomy effluent. Pouch Lactobacilli, Bifidobacterium, Bacteroides sp, and Clostridium perfringens counts were increased relative to ileostomy counts in patients with ulcerative colitis. Total pouch enterococci and coliform counts were also increased relative to ileostomy levels. There were no significant quantitative or qualitative differences between pouch types when these bacteria were evaluated. CONCLUSIONS: Sulfate-reducing bacteria are exclusive to patients with a background of ulcerative colitis. Not all ulcerative colitis pouches harbor sulfate-reducing bacteria because two ulcerative colitis pouches in this study were free of the latter. They are not present in familial adenomatous polyposis pouches or in ileostomy effluent collected from patients with ulcerative colitis. Total bacterial counts increase in ulcerative colitis pouches after stoma closure. Levels of Lactobacilli, Bifidobacterium, Bacteroides sp, Clostridium perfringens, enterococci, and coliforms were similar in both pouch groups. Because sulfate-reducing bacteria are specific to ulcerative colitis pouches, they may play a role in the pathogenesis of pouchitis.en_GB
dc.language.isoengen_GB
dc.subject.meshAdenomatous Polyposis Coli/*microbiology/physiopathology/*surgeryen_GB
dc.subject.meshAdulten_GB
dc.subject.meshBacteria, Aerobic/*isolation & purificationen_GB
dc.subject.meshBacteria, Anaerobic/*isolation & purificationen_GB
dc.subject.meshColitis, Ulcerative/*microbiology/physiopathology/*surgeryen_GB
dc.subject.meshColony Count, Microbialen_GB
dc.subject.meshDefecation/physiologyen_GB
dc.subject.meshFeces/microbiologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIleostomy/adverse effectsen_GB
dc.subject.meshMaleen_GB
dc.subject.meshPouchitis/*etiology/*microbiology/physiopathologyen_GB
dc.subject.meshProctocolectomy, Restorative/*adverse effectsen_GB
dc.subject.meshSulfates/*isolation & purificationen_GB
dc.titleSulfate-reducing bacteria colonize pouches formed for ulcerative colitis but not for familial adenomatous polyposis.en_GB
dc.contributor.departmentDepartment of Surgery, Cork University Hospital, Ireland.en_GB
dc.identifier.journalDiseases of the colon and rectumen_GB
dc.description.provinceMunster-

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