Effects of chronic occupational exposure to anaesthetic gases on the rate of neutrophil apoptosis among anaesthetists.

Hdl Handle:
http://hdl.handle.net/10147/209025
Title:
Effects of chronic occupational exposure to anaesthetic gases on the rate of neutrophil apoptosis among anaesthetists.
Authors:
Tyther, R; Halligan, M; Wang, J; Redmond, H P; Shorten, G
Affiliation:
Cork University Hospital, Departments of Anaesthesia and Intensive Care Medicine.
Citation:
Eur J Anaesthesiol. 2002 Aug;19(8):604-8.
Journal:
European journal of anaesthesiology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209025
PubMed ID:
12200952
Abstract:
BACKGROUND AND OBJECTIVE: Volatile anaesthetic agents are known to influence neutrophil function. The aim was to determine the effect of chronic occupational exposure to volatile anaesthetic agents on the rate of neutrophil apoptosis among anaesthetists. To test this hypothesis, we compared the rate of neutrophil apoptosis in anaesthetists who had been chronically exposed to volatile anaesthetic agents with that in unexposed volunteers. METHODS: Venous blood (20 mL) was withdrawn from 24 ASA I-II volunteers, from which neutrophils were isolated, and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide. RESULTS: At 1 h (but not at 12 and 24 h) in culture, the rate of neutrophil apoptosis was significantly less in the anaesthetists--13.8 (12.9%) versus 34.4 (12.1%) (P = 0.001). CONCLUSIONS: Chronic occupational exposure to volatile anaesthetic agents may inhibit neutrophil apoptosis. This may have implications for anaesthetists and similarly exposed healthcare workers in terms of the adequacy of their inflammatory response.
Language:
eng
MeSH:
Adult; Anesthetics, Inhalation/*pharmacology; Apoptosis/*drug effects; Cells, Cultured; Female; Flow Cytometry; Humans; Male; Middle Aged; Neutrophils/cytology/*drug effects; *Occupational Exposure; Time Factors
ISSN:
0265-0215 (Print); 0265-0215 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorTyther, Ren_GB
dc.contributor.authorHalligan, Men_GB
dc.contributor.authorWang, Jen_GB
dc.contributor.authorRedmond, H Pen_GB
dc.contributor.authorShorten, Gen_GB
dc.date.accessioned2012-02-03T15:10:14Z-
dc.date.available2012-02-03T15:10:14Z-
dc.date.issued2012-02-03T15:10:14Z-
dc.identifier.citationEur J Anaesthesiol. 2002 Aug;19(8):604-8.en_GB
dc.identifier.issn0265-0215 (Print)en_GB
dc.identifier.issn0265-0215 (Linking)en_GB
dc.identifier.pmid12200952en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209025-
dc.description.abstractBACKGROUND AND OBJECTIVE: Volatile anaesthetic agents are known to influence neutrophil function. The aim was to determine the effect of chronic occupational exposure to volatile anaesthetic agents on the rate of neutrophil apoptosis among anaesthetists. To test this hypothesis, we compared the rate of neutrophil apoptosis in anaesthetists who had been chronically exposed to volatile anaesthetic agents with that in unexposed volunteers. METHODS: Venous blood (20 mL) was withdrawn from 24 ASA I-II volunteers, from which neutrophils were isolated, and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide. RESULTS: At 1 h (but not at 12 and 24 h) in culture, the rate of neutrophil apoptosis was significantly less in the anaesthetists--13.8 (12.9%) versus 34.4 (12.1%) (P = 0.001). CONCLUSIONS: Chronic occupational exposure to volatile anaesthetic agents may inhibit neutrophil apoptosis. This may have implications for anaesthetists and similarly exposed healthcare workers in terms of the adequacy of their inflammatory response.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAnesthetics, Inhalation/*pharmacologyen_GB
dc.subject.meshApoptosis/*drug effectsen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshFemaleen_GB
dc.subject.meshFlow Cytometryen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshNeutrophils/cytology/*drug effectsen_GB
dc.subject.mesh*Occupational Exposureen_GB
dc.subject.meshTime Factorsen_GB
dc.titleEffects of chronic occupational exposure to anaesthetic gases on the rate of neutrophil apoptosis among anaesthetists.en_GB
dc.contributor.departmentCork University Hospital, Departments of Anaesthesia and Intensive Care Medicine.en_GB
dc.identifier.journalEuropean journal of anaesthesiologyen_GB
dc.description.provinceMunster-

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