Effect of sevoflurane on human neutrophil apoptosis.

Hdl Handle:
http://hdl.handle.net/10147/209008
Title:
Effect of sevoflurane on human neutrophil apoptosis.
Authors:
Tyther, R; O'Brien, J; Wang, J; Redmond, H P; Shorten, G
Affiliation:
Cork University Hospital, Department of Anaesthesia and Intensive Care Medicine, , Cork, Ireland.
Citation:
Eur J Anaesthesiol. 2003 Feb;20(2):111-5.
Journal:
European journal of anaesthesiology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/209008
PubMed ID:
12622493
Abstract:
BACKGROUND AND OBJECTIVE: Both chronic occupational exposure to volatile anaesthetic agents and acute in vitro exposure of neutrophils to isoflurane have been shown to inhibit the rate of apoptosis of human neutrophils. It is possible that inhibition of neutrophil apoptosis arises through delaying mitochondrial membrane potential collapse. We assessed mitochondrial depolarization and apoptosis in unexposed neutrophils and neutrophils exposed to sevoflurane in vivo. METHODS: A total of 20 mL venous blood was withdrawn pre- and postinduction of anaesthesia, the neutrophils isolated and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide. Mitochondrial depolarization was measured using the dual emission styryl dye JC-1. RESULTS: Apoptosis was significantly inhibited in neutrophils exposed to sevoflurane in vivo at 24 (exposed: 38 (12)% versus control: 28 (11)%, P = 0.001), but not at 1 or 12 h, in culture. Mitochondrial depolarization was not delayed in neutrophils exposed to sevoflurane. CONCLUSIONS: The most important findings are that sevoflurane inhibits neutrophil apoptosis in vivo and that inhibition is not mediated primarily by an effect on mitochondrial depolarization.
Language:
eng
MeSH:
Anesthetics, Inhalation/blood/*pharmacology; Annexins/metabolism; Apoptosis/*drug effects; Cells, Cultured; Flow Cytometry; Humans; Methyl Ethers/blood/*pharmacology; Neutrophils/*drug effects; Surgical Procedures, Elective; Time Factors
ISSN:
0265-0215 (Print); 0265-0215 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorTyther, Ren_GB
dc.contributor.authorO'Brien, Jen_GB
dc.contributor.authorWang, Jen_GB
dc.contributor.authorRedmond, H Pen_GB
dc.contributor.authorShorten, Gen_GB
dc.date.accessioned2012-02-03T15:09:47Z-
dc.date.available2012-02-03T15:09:47Z-
dc.date.issued2012-02-03T15:09:47Z-
dc.identifier.citationEur J Anaesthesiol. 2003 Feb;20(2):111-5.en_GB
dc.identifier.issn0265-0215 (Print)en_GB
dc.identifier.issn0265-0215 (Linking)en_GB
dc.identifier.pmid12622493en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209008-
dc.description.abstractBACKGROUND AND OBJECTIVE: Both chronic occupational exposure to volatile anaesthetic agents and acute in vitro exposure of neutrophils to isoflurane have been shown to inhibit the rate of apoptosis of human neutrophils. It is possible that inhibition of neutrophil apoptosis arises through delaying mitochondrial membrane potential collapse. We assessed mitochondrial depolarization and apoptosis in unexposed neutrophils and neutrophils exposed to sevoflurane in vivo. METHODS: A total of 20 mL venous blood was withdrawn pre- and postinduction of anaesthesia, the neutrophils isolated and maintained in culture. At 1, 12 and 24 h in culture, the percentage of neutrophil apoptosis was assessed by dual staining with annexin V-FITC and propidium iodide. Mitochondrial depolarization was measured using the dual emission styryl dye JC-1. RESULTS: Apoptosis was significantly inhibited in neutrophils exposed to sevoflurane in vivo at 24 (exposed: 38 (12)% versus control: 28 (11)%, P = 0.001), but not at 1 or 12 h, in culture. Mitochondrial depolarization was not delayed in neutrophils exposed to sevoflurane. CONCLUSIONS: The most important findings are that sevoflurane inhibits neutrophil apoptosis in vivo and that inhibition is not mediated primarily by an effect on mitochondrial depolarization.en_GB
dc.language.isoengen_GB
dc.subject.meshAnesthetics, Inhalation/blood/*pharmacologyen_GB
dc.subject.meshAnnexins/metabolismen_GB
dc.subject.meshApoptosis/*drug effectsen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshFlow Cytometryen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMethyl Ethers/blood/*pharmacologyen_GB
dc.subject.meshNeutrophils/*drug effectsen_GB
dc.subject.meshSurgical Procedures, Electiveen_GB
dc.subject.meshTime Factorsen_GB
dc.titleEffect of sevoflurane on human neutrophil apoptosis.en_GB
dc.contributor.departmentCork University Hospital, Department of Anaesthesia and Intensive Care Medicine, , Cork, Ireland.en_GB
dc.identifier.journalEuropean journal of anaesthesiologyen_GB
dc.description.provinceMunster-

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