Activated endothelial interleukin-1beta, -6, and -8 concentrations and intercellular adhesion molecule-1 expression are attenuated by lidocaine.

Hdl Handle:
http://hdl.handle.net/10147/208931
Title:
Activated endothelial interleukin-1beta, -6, and -8 concentrations and intercellular adhesion molecule-1 expression are attenuated by lidocaine.
Authors:
Lan, Wei; Harmon, Dominic C; Wang, Jiang H; Shorten, George D; Redmond, Paul H
Affiliation:
Academic Department of Surgery, Cork University Hospital, University College,, Cork, Ireland. dweilan@hotmail.com
Citation:
Anesth Analg. 2005 Feb;100(2):409-12.
Journal:
Anesthesia and analgesia
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/208931
DOI:
10.1213/01.ANE.0000142113.39092.87
PubMed ID:
15673867
Abstract:
Endothelial cells play a key role in ischemia reperfusion injury. We investigated the effects of lidocaine on activated human umbilical vein endothelial cell (HUVEC) interleukin (IL)-1beta, IL-6, and IL-8 concentrations and intercellular adhesion molecule-1 (ICAM-1) expression. HUVECs were pretreated with different concentrations of lidocaine (0 to 0.5 mg/mL) for 60 min, thereafter tumor necrosis factor-alpha was added at a concentration of 2.5 ng/mL and the cells incubated for 4 h. Supernatants were harvested, and cytokine concentrations were analyzed by enzyme-linked immunosorbent assay. Endothelial ICAM-1 expression was analyzed by using flow cytometry. Differences were assessed using analysis of variance and post hoc unpaired Student's t-test where appropriate. Lidocaine (0.5 mg/mL) decreased IL-1beta (1.89 +/- 0.11 versus 4.16 +/- 1.27 pg/mL; P = 0.009), IL-6 (65.5 +/- 5.14 versus 162 +/- 11.5 pg/mL; P < 0.001), and IL-8 (3869 +/- 785 versus 14,961 +/- 406 pg/mL; P < 0.001) concentrations compared with the control. IL-1beta, IL-6, and IL-8 concentrations in HUVECs treated with clinically relevant plasma concentrations of lidocaine (0.005 mg/mL) were similar to control. ICAM-1 expression on lidocaine-treated (0.05 mg/mL) HUVECs was less than on controls (198 +/- 52.7 versus 298 +/- 50.3; Mean Channel Fluorescence; P < 0.001). Activated endothelial IL-1beta, IL-6, and IL-8 concentrations and ICAM-1 expression are attenuated only by lidocaine at concentrations larger than clinically relevant concentrations.
Language:
eng
MeSH:
Anesthetics, Local/*pharmacology; Endothelial Cells/drug effects/metabolism; Enzyme-Linked Immunosorbent Assay; Humans; Intercellular Adhesion Molecule-1/*biosynthesis; Interleukin-1/*metabolism; Interleukin-6/*metabolism; Interleukin-8/*metabolism; Lidocaine/*pharmacology; Tumor Necrosis Factor-alpha/biosynthesis
ISSN:
0003-2999 (Print); 0003-2999 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorLan, Weien_GB
dc.contributor.authorHarmon, Dominic Cen_GB
dc.contributor.authorWang, Jiang Hen_GB
dc.contributor.authorShorten, George Den_GB
dc.contributor.authorRedmond, Paul Hen_GB
dc.date.accessioned2012-02-03T15:07:44Z-
dc.date.available2012-02-03T15:07:44Z-
dc.date.issued2012-02-03T15:07:44Z-
dc.identifier.citationAnesth Analg. 2005 Feb;100(2):409-12.en_GB
dc.identifier.issn0003-2999 (Print)en_GB
dc.identifier.issn0003-2999 (Linking)en_GB
dc.identifier.pmid15673867en_GB
dc.identifier.doi10.1213/01.ANE.0000142113.39092.87en_GB
dc.identifier.urihttp://hdl.handle.net/10147/208931-
dc.description.abstractEndothelial cells play a key role in ischemia reperfusion injury. We investigated the effects of lidocaine on activated human umbilical vein endothelial cell (HUVEC) interleukin (IL)-1beta, IL-6, and IL-8 concentrations and intercellular adhesion molecule-1 (ICAM-1) expression. HUVECs were pretreated with different concentrations of lidocaine (0 to 0.5 mg/mL) for 60 min, thereafter tumor necrosis factor-alpha was added at a concentration of 2.5 ng/mL and the cells incubated for 4 h. Supernatants were harvested, and cytokine concentrations were analyzed by enzyme-linked immunosorbent assay. Endothelial ICAM-1 expression was analyzed by using flow cytometry. Differences were assessed using analysis of variance and post hoc unpaired Student's t-test where appropriate. Lidocaine (0.5 mg/mL) decreased IL-1beta (1.89 +/- 0.11 versus 4.16 +/- 1.27 pg/mL; P = 0.009), IL-6 (65.5 +/- 5.14 versus 162 +/- 11.5 pg/mL; P < 0.001), and IL-8 (3869 +/- 785 versus 14,961 +/- 406 pg/mL; P < 0.001) concentrations compared with the control. IL-1beta, IL-6, and IL-8 concentrations in HUVECs treated with clinically relevant plasma concentrations of lidocaine (0.005 mg/mL) were similar to control. ICAM-1 expression on lidocaine-treated (0.05 mg/mL) HUVECs was less than on controls (198 +/- 52.7 versus 298 +/- 50.3; Mean Channel Fluorescence; P < 0.001). Activated endothelial IL-1beta, IL-6, and IL-8 concentrations and ICAM-1 expression are attenuated only by lidocaine at concentrations larger than clinically relevant concentrations.en_GB
dc.language.isoengen_GB
dc.subject.meshAnesthetics, Local/*pharmacologyen_GB
dc.subject.meshEndothelial Cells/drug effects/metabolismen_GB
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIntercellular Adhesion Molecule-1/*biosynthesisen_GB
dc.subject.meshInterleukin-1/*metabolismen_GB
dc.subject.meshInterleukin-6/*metabolismen_GB
dc.subject.meshInterleukin-8/*metabolismen_GB
dc.subject.meshLidocaine/*pharmacologyen_GB
dc.subject.meshTumor Necrosis Factor-alpha/biosynthesisen_GB
dc.titleActivated endothelial interleukin-1beta, -6, and -8 concentrations and intercellular adhesion molecule-1 expression are attenuated by lidocaine.en_GB
dc.contributor.departmentAcademic Department of Surgery, Cork University Hospital, University College,, Cork, Ireland. dweilan@hotmail.comen_GB
dc.identifier.journalAnesthesia and analgesiaen_GB
dc.description.provinceMunster-
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