Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a.
Affiliation
Molecular Virology Diagnostic & Research Laboratory, Department of Medicine,, Clinical Sciences Building, Cork University Hospital, Cork, Ireland., i.moreau@ucc.ieIssue Date
2012-02-03T15:07:41ZMeSH
AdultAntiviral Agents/administration & dosage/*therapeutic use
Drug Therapy, Combination
Evolution, Molecular
Female
*Genetic Variation
Genotype
Hepacivirus/*classification/drug effects/genetics
Hepatitis C, Chronic/*drug therapy/virology
Humans
Interferon-alpha/administration & dosage/*therapeutic use
Male
Middle Aged
Molecular Sequence Data
Phylogeny
Polyethylene Glycols/administration & dosage/*therapeutic use
Recombinant Proteins
Reverse Transcriptase Polymerase Chain Reaction
Ribavirin/administration & dosage/*therapeutic use
Sequence Analysis, DNA
Treatment Failure
Treatment Outcome
Viral Load
Virus Replication
Metadata
Show full item recordCitation
Virol J. 2008 Jul 9;5:78.Journal
Virology journalDOI
10.1186/1743-422X-5-78PubMed ID
18613968Abstract
Pre-treatment HCV quasispecies complexity and diversity may predict response to interferon based anti-viral therapy. The objective of this study was to retrospectively (1) examine temporal changes in quasispecies prior to the start of therapy and (2) investigate extensively quasispecies evolution in a group of 10 chronically infected patients with genotype 3a, treated with pegylated alpha2a-Interferon and ribavirin. The degree of sequence heterogeneity within the hypervariable region 1 was assessed by analyzing 20-30 individual clones in serial serum samples. Genetic parameters, including amino acid Shannon entropy, Hamming distance and genetic distance were calculated for each sample. Treatment outcome was divided into (1) sustained virological responders (SVR) and (2) treatment failure (TF). Our results indicate, (1) quasispecies complexity and diversity are lower in the SVR group, (2) quasispecies vary temporally and (3) genetic heterogeneity at baseline can be use to predict treatment outcome. We discuss the results from the perspective of replicative homeostasis.Language
engISSN
1743-422X (Electronic)1743-422X (Linking)
ae974a485f413a2113503eed53cd6c53
10.1186/1743-422X-5-78