The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM-1 expression and IL-1beta concentrations induced by hypoxia-reoxygenation.

Hdl Handle:
http://hdl.handle.net/10147/208924
Title:
The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM-1 expression and IL-1beta concentrations induced by hypoxia-reoxygenation.
Authors:
Lan, W; Harmon, D; Wang, J H; Shorten, G; Redmond, P
Affiliation:
Cork University Hospital, Academic Department of Surgery, Cork, Ireland., dweilan@hotmail.com
Citation:
Eur J Anaesthesiol. 2004 Dec;21(12):967-72.
Journal:
European journal of anaesthesiology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/208924
PubMed ID:
15719860
Abstract:
BACKGROUND: Lidocaine has actions potentially of benefit during ischaemia-reperfusion. Neutrophils and endothelial cells have an important role in ischaemia-reperfusion injury. METHODS: Isolated human neutrophil CD11b and CD18, and human umbilical vein endothelial cell (HUVEC) ICAM-1 expression and supernatant IL-1beta concentrations in response to hypoxia-reoxygenation were studied in the presence or absence of different concentrations of lidocaine (0.005, 0.05 and 0.5 mg mL(-1)). Adhesion molecule expression was quantified by flow cytometry and IL- 1beta concentrations by ELISA. Differences were assessed with analysis of variance and Student-Newman-Keuls as appropriate. Data are presented as mean+/-SD. RESULTS: Exposure to hypoxia-reoxygenation increased neutrophil CD11b (94.33+/-40.65 vs. 34.32+/-6.83 mean channel fluorescence (MCF), P = 0.02), CD18 (109.84+/-35.44 vs. 59.05+/-6.71 MCF, P = 0.03) and endothelial ICAM-1 (146.62+/-16.78 vs. 47.29+/-9.85 MCF, P < 0.001) expression compared to normoxia. Neutrophil CD18 expression on exposure to hypoxia-reoxygenation was less in lidocaine (0.005 mg mL(-1)) treated cells compared to control (71.07+/-10.14 vs. 109.84+/-35.44 MCF, P = 0.03). Endothelial ICAM-1 expression on exposure to hypoxia-reoxygenation was less in lidocaine (0.005 mg mL(-1)) treated cells compared to control (133.25+/-16.05 vs. 146.62+/-16.78 MCF, P = 0.03). Hypoxia-reoxygenation increased HUVEC supernatant IL-1beta concentrations compared to normoxia (3.41+/-0.36 vs. 2.65+/-0.21 pg mL(-1), P = 0.02). Endothelial supernatant IL-1beta concentrations in lidocaine-treated HUVECs were similar to controls. CONCLUSIONS: Lidocaine at clinically relevant concentrations decreased neutrophil CD18 and endothelial ICAM-1 expression but not endothelial IL-1beta concentrations.
Language:
eng
MeSH:
Analysis of Variance; Anesthetics, Local/pharmacology; Antigens, CD11b/*drug effects/metabolism; Antigens, CD18/*drug effects/metabolism; Cell Hypoxia/drug effects/physiology; Cells, Cultured; Dose-Response Relationship, Drug; Endothelial Cells/*drug effects/metabolism; Enzyme-Linked Immunosorbent Assay/methods; Flow Cytometry/methods; Humans; Intercellular Adhesion Molecule-1/*drug effects/metabolism; Interleukin-1/metabolism; Interleukin-1beta; Lidocaine/*pharmacology; Neutrophils/*drug effects/metabolism; Peptide Fragments/*drug effects/metabolism; Reference Values; Reperfusion/methods; Reperfusion Injury/metabolism; Umbilical Veins
ISSN:
0265-0215 (Print); 0265-0215 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorLan, Wen_GB
dc.contributor.authorHarmon, Den_GB
dc.contributor.authorWang, J Hen_GB
dc.contributor.authorShorten, Gen_GB
dc.contributor.authorRedmond, Pen_GB
dc.date.accessioned2012-02-03T15:07:33Z-
dc.date.available2012-02-03T15:07:33Z-
dc.date.issued2012-02-03T15:07:33Z-
dc.identifier.citationEur J Anaesthesiol. 2004 Dec;21(12):967-72.en_GB
dc.identifier.issn0265-0215 (Print)en_GB
dc.identifier.issn0265-0215 (Linking)en_GB
dc.identifier.pmid15719860en_GB
dc.identifier.urihttp://hdl.handle.net/10147/208924-
dc.description.abstractBACKGROUND: Lidocaine has actions potentially of benefit during ischaemia-reperfusion. Neutrophils and endothelial cells have an important role in ischaemia-reperfusion injury. METHODS: Isolated human neutrophil CD11b and CD18, and human umbilical vein endothelial cell (HUVEC) ICAM-1 expression and supernatant IL-1beta concentrations in response to hypoxia-reoxygenation were studied in the presence or absence of different concentrations of lidocaine (0.005, 0.05 and 0.5 mg mL(-1)). Adhesion molecule expression was quantified by flow cytometry and IL- 1beta concentrations by ELISA. Differences were assessed with analysis of variance and Student-Newman-Keuls as appropriate. Data are presented as mean+/-SD. RESULTS: Exposure to hypoxia-reoxygenation increased neutrophil CD11b (94.33+/-40.65 vs. 34.32+/-6.83 mean channel fluorescence (MCF), P = 0.02), CD18 (109.84+/-35.44 vs. 59.05+/-6.71 MCF, P = 0.03) and endothelial ICAM-1 (146.62+/-16.78 vs. 47.29+/-9.85 MCF, P < 0.001) expression compared to normoxia. Neutrophil CD18 expression on exposure to hypoxia-reoxygenation was less in lidocaine (0.005 mg mL(-1)) treated cells compared to control (71.07+/-10.14 vs. 109.84+/-35.44 MCF, P = 0.03). Endothelial ICAM-1 expression on exposure to hypoxia-reoxygenation was less in lidocaine (0.005 mg mL(-1)) treated cells compared to control (133.25+/-16.05 vs. 146.62+/-16.78 MCF, P = 0.03). Hypoxia-reoxygenation increased HUVEC supernatant IL-1beta concentrations compared to normoxia (3.41+/-0.36 vs. 2.65+/-0.21 pg mL(-1), P = 0.02). Endothelial supernatant IL-1beta concentrations in lidocaine-treated HUVECs were similar to controls. CONCLUSIONS: Lidocaine at clinically relevant concentrations decreased neutrophil CD18 and endothelial ICAM-1 expression but not endothelial IL-1beta concentrations.en_GB
dc.language.isoengen_GB
dc.subject.meshAnalysis of Varianceen_GB
dc.subject.meshAnesthetics, Local/pharmacologyen_GB
dc.subject.meshAntigens, CD11b/*drug effects/metabolismen_GB
dc.subject.meshAntigens, CD18/*drug effects/metabolismen_GB
dc.subject.meshCell Hypoxia/drug effects/physiologyen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshDose-Response Relationship, Drugen_GB
dc.subject.meshEndothelial Cells/*drug effects/metabolismen_GB
dc.subject.meshEnzyme-Linked Immunosorbent Assay/methodsen_GB
dc.subject.meshFlow Cytometry/methodsen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIntercellular Adhesion Molecule-1/*drug effects/metabolismen_GB
dc.subject.meshInterleukin-1/metabolismen_GB
dc.subject.meshInterleukin-1betaen_GB
dc.subject.meshLidocaine/*pharmacologyen_GB
dc.subject.meshNeutrophils/*drug effects/metabolismen_GB
dc.subject.meshPeptide Fragments/*drug effects/metabolismen_GB
dc.subject.meshReference Valuesen_GB
dc.subject.meshReperfusion/methodsen_GB
dc.subject.meshReperfusion Injury/metabolismen_GB
dc.subject.meshUmbilical Veinsen_GB
dc.titleThe effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM-1 expression and IL-1beta concentrations induced by hypoxia-reoxygenation.en_GB
dc.contributor.departmentCork University Hospital, Academic Department of Surgery, Cork, Ireland., dweilan@hotmail.comen_GB
dc.identifier.journalEuropean journal of anaesthesiologyen_GB
dc.description.provinceMunster-
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