A characterization of anaerobic colonization and associated mucosal adaptations in the undiseased ileal pouch.

Hdl Handle:
http://hdl.handle.net/10147/208885
Title:
A characterization of anaerobic colonization and associated mucosal adaptations in the undiseased ileal pouch.
Authors:
Smith, F M; Coffey, J C; Kell, M R; O'Sullivan, M; Redmond, H P; Kirwan, W O
Affiliation:
Department of Surgery University College Cork and Cork University Hospital,, National University of Ireland.
Citation:
Colorectal Dis. 2005 Nov;7(6):563-70.
Journal:
Colorectal disease : the official journal of the Association of Coloproctology of, Great Britain and Ireland
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/208885
DOI:
10.1111/j.1463-1318.2005.00833.x
PubMed ID:
16232236
Abstract:
INTRODUCTION: The resolution of pouchitis with metronidazole points to an anaerobic aetiology. Pouchitis is mainly seen in patients with ulcerative colitis pouches (UCP). We have recently found that sulphate reducing bacteria (SRB), a species of strict anaerobe, colonize UCP exclusively. Herein, we aimed to correlate levels of different bacterial species (including SRB) with mucosal inflammation and morphology. METHODS: Following ethical approval, fresh faecal samples and mucosal biopsies were taken from 9 patients with UCP and 5 patients with familial adenomatous polyposis pouches (FAPP). For the purposes of comparison, faecal samples and mucosal biopsies were also taken from the stomas of 7 of the 9 patients with UC (UCS). Colonization by four types of strict anaerobes (SRB, Clostridium perfringens, Bifidobacteria and Bacteroides) as well as by three types of facultative anaerobes (Enterococci, Coliforms and Lactobacilli) was evaluated. Inflammatory scores and mucosal morphology were assessed histologically in a blinded fashion by a pathologist. RESULTS: In general, strict anaerobes predominated over facultative in the UCP (P = 0.041). SRB were present in UCP exclusively. Even after exclusion of SRB from total bacterial counts, strict anaerobes still predominated. In the UCS, facultative anaerobes predominated. Strict and facultative anaerobes were present at similar levels in the FAPP. Enterococci were present at significantly reduced levels in the UCP when compared with the UCS (P = 0.031). When levels of SRB and other anaerobic species were individually correlated with mucosal inflammation and morphology, no trends were observed. CONCLUSION: We have previously identified that SRB exclusively colonize UCP. In addition we have now identified a novel increase in the strict/facultative anaerobic ratio within the UCP compared to UCS. These stark differences in bacterial colonization, however, appear to have limited impact on mucosal inflammation or morphology.
Language:
eng
MeSH:
Adenomatous Polyposis Coli/surgery; Bacteroides/*isolation & purification; Bifidobacterium/*isolation & purification; Clostridium perfringens/*isolation & purification; Colitis, Ulcerative/surgery; Female; Humans; Intestinal Mucosa/*pathology; Male; Pouchitis/*microbiology/*pathology
ISSN:
1462-8910 (Print); 1462-8910 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorSmith, F Men_GB
dc.contributor.authorCoffey, J Cen_GB
dc.contributor.authorKell, M Ren_GB
dc.contributor.authorO'Sullivan, Men_GB
dc.contributor.authorRedmond, H Pen_GB
dc.contributor.authorKirwan, W Oen_GB
dc.date.accessioned2012-02-03T15:06:27Z-
dc.date.available2012-02-03T15:06:27Z-
dc.date.issued2012-02-03T15:06:27Z-
dc.identifier.citationColorectal Dis. 2005 Nov;7(6):563-70.en_GB
dc.identifier.issn1462-8910 (Print)en_GB
dc.identifier.issn1462-8910 (Linking)en_GB
dc.identifier.pmid16232236en_GB
dc.identifier.doi10.1111/j.1463-1318.2005.00833.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/208885-
dc.description.abstractINTRODUCTION: The resolution of pouchitis with metronidazole points to an anaerobic aetiology. Pouchitis is mainly seen in patients with ulcerative colitis pouches (UCP). We have recently found that sulphate reducing bacteria (SRB), a species of strict anaerobe, colonize UCP exclusively. Herein, we aimed to correlate levels of different bacterial species (including SRB) with mucosal inflammation and morphology. METHODS: Following ethical approval, fresh faecal samples and mucosal biopsies were taken from 9 patients with UCP and 5 patients with familial adenomatous polyposis pouches (FAPP). For the purposes of comparison, faecal samples and mucosal biopsies were also taken from the stomas of 7 of the 9 patients with UC (UCS). Colonization by four types of strict anaerobes (SRB, Clostridium perfringens, Bifidobacteria and Bacteroides) as well as by three types of facultative anaerobes (Enterococci, Coliforms and Lactobacilli) was evaluated. Inflammatory scores and mucosal morphology were assessed histologically in a blinded fashion by a pathologist. RESULTS: In general, strict anaerobes predominated over facultative in the UCP (P = 0.041). SRB were present in UCP exclusively. Even after exclusion of SRB from total bacterial counts, strict anaerobes still predominated. In the UCS, facultative anaerobes predominated. Strict and facultative anaerobes were present at similar levels in the FAPP. Enterococci were present at significantly reduced levels in the UCP when compared with the UCS (P = 0.031). When levels of SRB and other anaerobic species were individually correlated with mucosal inflammation and morphology, no trends were observed. CONCLUSION: We have previously identified that SRB exclusively colonize UCP. In addition we have now identified a novel increase in the strict/facultative anaerobic ratio within the UCP compared to UCS. These stark differences in bacterial colonization, however, appear to have limited impact on mucosal inflammation or morphology.en_GB
dc.language.isoengen_GB
dc.subject.meshAdenomatous Polyposis Coli/surgeryen_GB
dc.subject.meshBacteroides/*isolation & purificationen_GB
dc.subject.meshBifidobacterium/*isolation & purificationen_GB
dc.subject.meshClostridium perfringens/*isolation & purificationen_GB
dc.subject.meshColitis, Ulcerative/surgeryen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIntestinal Mucosa/*pathologyen_GB
dc.subject.meshMaleen_GB
dc.subject.meshPouchitis/*microbiology/*pathologyen_GB
dc.titleA characterization of anaerobic colonization and associated mucosal adaptations in the undiseased ileal pouch.en_GB
dc.contributor.departmentDepartment of Surgery University College Cork and Cork University Hospital,, National University of Ireland.en_GB
dc.identifier.journalColorectal disease : the official journal of the Association of Coloproctology of, Great Britain and Irelanden_GB
dc.description.provinceMunster-

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