The influence of propofol on P-selectin expression and nitric oxide production in re-oxygenated human umbilical vein endothelial cells.

Hdl Handle:
http://hdl.handle.net/10147/208861
Title:
The influence of propofol on P-selectin expression and nitric oxide production in re-oxygenated human umbilical vein endothelial cells.
Authors:
Corcoran, T B; O'Shea, A; Engel, A; Shorten, G D
Affiliation:
Department of Anaesthesia, Cork University Hospital, Cork City, Republic of, Ireland. mascor@gofree.indigo.ie
Citation:
Acta Anaesthesiol Scand. 2006 Mar;50(3):348-54.
Journal:
Acta anaesthesiologica Scandinavica
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/208861
DOI:
10.1111/j.1399-6576.2006.00955.x
PubMed ID:
16480469
Abstract:
BACKGROUND: Reperfusion injury is characterized by free radical production and endothelial inflammation. Neutrophils mediate much of the end-organ injury that occurs, requiring P-selectin-mediated neutrophil-endothelial adhesion, and this is associated with decreased endothelial nitric oxide production. Propofol has antioxidant properties in vitro which might abrogate this inflammation. METHODS: Cultured human umbilical vein endothelial cells were exposed to 20 h of hypoxia and then returned to normoxic conditions. Cells were treated with saline, Diprivan 5 microg/l or propofol 5 microg/l for 4 h after re-oxygenation and were then examined for P-selectin expression and supernatant nitric oxide concentrations for 24 h. P-selectin was determined by flow cytometry, and culture supernatant nitric oxide was measured as nitrite. RESULTS: In saline-treated cells, a biphasic increase in P-selectin expression was demonstrated at 30 min (P = 0.01) and 4 h (P = 0.023) after re-oxygenation. Propofol and Diprivan prevented these increases in P-selectin expression (P < 0.05). Four hours after re-oxygenation, propofol decreased endothelial nitric oxide production (P = 0.035). CONCLUSION: This is the first study to demonstrate an effect of propofol upon endothelial P-selectin expression. Such an effect may be important in situations of reperfusion injury such as cardiac transplantation and coronary artery bypass surgery. We conclude that propofol attenuates re-oxygenation-induced endothelial inflammation in vitro.
Language:
eng
MeSH:
Antioxidants/pharmacology; Cell Hypoxia; Cells, Cultured; Endothelial Cells/*drug effects/metabolism; Humans; Nitric Oxide/*biosynthesis; Nitric Oxide Synthase Type III/analysis; Oxygen/*pharmacology; P-Selectin/*analysis; Propofol/*pharmacology; Reperfusion Injury/prevention & control
ISSN:
0001-5172 (Print); 0001-5172 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorCorcoran, T Ben_GB
dc.contributor.authorO'Shea, Aen_GB
dc.contributor.authorEngel, Aen_GB
dc.contributor.authorShorten, G Den_GB
dc.date.accessioned2012-02-03T15:05:44Z-
dc.date.available2012-02-03T15:05:44Z-
dc.date.issued2012-02-03T15:05:44Z-
dc.identifier.citationActa Anaesthesiol Scand. 2006 Mar;50(3):348-54.en_GB
dc.identifier.issn0001-5172 (Print)en_GB
dc.identifier.issn0001-5172 (Linking)en_GB
dc.identifier.pmid16480469en_GB
dc.identifier.doi10.1111/j.1399-6576.2006.00955.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/208861-
dc.description.abstractBACKGROUND: Reperfusion injury is characterized by free radical production and endothelial inflammation. Neutrophils mediate much of the end-organ injury that occurs, requiring P-selectin-mediated neutrophil-endothelial adhesion, and this is associated with decreased endothelial nitric oxide production. Propofol has antioxidant properties in vitro which might abrogate this inflammation. METHODS: Cultured human umbilical vein endothelial cells were exposed to 20 h of hypoxia and then returned to normoxic conditions. Cells were treated with saline, Diprivan 5 microg/l or propofol 5 microg/l for 4 h after re-oxygenation and were then examined for P-selectin expression and supernatant nitric oxide concentrations for 24 h. P-selectin was determined by flow cytometry, and culture supernatant nitric oxide was measured as nitrite. RESULTS: In saline-treated cells, a biphasic increase in P-selectin expression was demonstrated at 30 min (P = 0.01) and 4 h (P = 0.023) after re-oxygenation. Propofol and Diprivan prevented these increases in P-selectin expression (P < 0.05). Four hours after re-oxygenation, propofol decreased endothelial nitric oxide production (P = 0.035). CONCLUSION: This is the first study to demonstrate an effect of propofol upon endothelial P-selectin expression. Such an effect may be important in situations of reperfusion injury such as cardiac transplantation and coronary artery bypass surgery. We conclude that propofol attenuates re-oxygenation-induced endothelial inflammation in vitro.en_GB
dc.language.isoengen_GB
dc.subject.meshAntioxidants/pharmacologyen_GB
dc.subject.meshCell Hypoxiaen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshEndothelial Cells/*drug effects/metabolismen_GB
dc.subject.meshHumansen_GB
dc.subject.meshNitric Oxide/*biosynthesisen_GB
dc.subject.meshNitric Oxide Synthase Type III/analysisen_GB
dc.subject.meshOxygen/*pharmacologyen_GB
dc.subject.meshP-Selectin/*analysisen_GB
dc.subject.meshPropofol/*pharmacologyen_GB
dc.subject.meshReperfusion Injury/prevention & controlen_GB
dc.titleThe influence of propofol on P-selectin expression and nitric oxide production in re-oxygenated human umbilical vein endothelial cells.en_GB
dc.contributor.departmentDepartment of Anaesthesia, Cork University Hospital, Cork City, Republic of, Ireland. mascor@gofree.indigo.ieen_GB
dc.identifier.journalActa anaesthesiologica Scandinavicaen_GB
dc.description.provinceMunster-
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