Bcl-x(L) expression in vivo in rheumatoid synovium.

Hdl Handle:
http://hdl.handle.net/10147/208853
Title:
Bcl-x(L) expression in vivo in rheumatoid synovium.
Authors:
Busteed, S; Bennett, M W; Molloy, C; Houston, A; Stone, M A; Shanahan, F; Molloy, M G; O'Connell, J
Affiliation:
Department of Medicine, Cork University Hospital, National University of Ireland,, Wilton, Cork, Ireland. sandra_busteed@hotmail.com
Citation:
Clin Rheumatol. 2006 Nov;25(6):789-93. Epub 2006 Mar 30.
Journal:
Clinical rheumatology
Issue Date:
3-Feb-2012
URI:
http://hdl.handle.net/10147/208853
DOI:
10.1007/s10067-005-0191-0
PubMed ID:
16572289
Abstract:
To examine the expression of the apoptosis regulatory protein, Bcl-x(L), in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemistry for Bcl-x(L) was carried out on synovial samples from patients with RA and OA. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to qualitatively examine the expression of Bcl-x(L). Bcl-x(L) expression was detected in the lining, endothelium and inflammatory cells of both RA (n=20) and OA (n=10) samples. However, there was significantly more expression in the lining of RA synovium compared to OA (77 vs 61%, p<0.05). Many of the positive cells in the RA subsynovium were noted to be plasma cells. There was a significant correlation between Bcl-x(L) expression and the number of inflammatory cells in the subsynovium of RA and OA patients (r (s)=0.376, p<0.05, n=30). Age and disease duration did not correlate with Bcl-x(L) expression in rheumatoid patients. Bcl-x(L) may play a role in the extended survival of synoviocytes and inflammatory cells in rheumatoid synovium.
Language:
eng
MeSH:
Aged; Aging/metabolism; Arthritis, Rheumatoid/*metabolism/pathology; Blotting, Western; Endothelial Cells/metabolism; Female; Humans; Immunohistochemistry; Male; Middle Aged; Osteoarthritis/metabolism/pathology; Plasma Cells/metabolism; RNA, Messenger/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Staining and Labeling; Synovial Membrane/*metabolism/pathology; Time Factors; bcl-X Protein/genetics/*metabolism
ISSN:
0770-3198 (Print); 0770-3198 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorBusteed, Sen_GB
dc.contributor.authorBennett, M Wen_GB
dc.contributor.authorMolloy, Cen_GB
dc.contributor.authorHouston, Aen_GB
dc.contributor.authorStone, M Aen_GB
dc.contributor.authorShanahan, Fen_GB
dc.contributor.authorMolloy, M Gen_GB
dc.contributor.authorO'Connell, Jen_GB
dc.date.accessioned2012-02-03T15:05:30Z-
dc.date.available2012-02-03T15:05:30Z-
dc.date.issued2012-02-03T15:05:30Z-
dc.identifier.citationClin Rheumatol. 2006 Nov;25(6):789-93. Epub 2006 Mar 30.en_GB
dc.identifier.issn0770-3198 (Print)en_GB
dc.identifier.issn0770-3198 (Linking)en_GB
dc.identifier.pmid16572289en_GB
dc.identifier.doi10.1007/s10067-005-0191-0en_GB
dc.identifier.urihttp://hdl.handle.net/10147/208853-
dc.description.abstractTo examine the expression of the apoptosis regulatory protein, Bcl-x(L), in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemistry for Bcl-x(L) was carried out on synovial samples from patients with RA and OA. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to qualitatively examine the expression of Bcl-x(L). Bcl-x(L) expression was detected in the lining, endothelium and inflammatory cells of both RA (n=20) and OA (n=10) samples. However, there was significantly more expression in the lining of RA synovium compared to OA (77 vs 61%, p<0.05). Many of the positive cells in the RA subsynovium were noted to be plasma cells. There was a significant correlation between Bcl-x(L) expression and the number of inflammatory cells in the subsynovium of RA and OA patients (r (s)=0.376, p<0.05, n=30). Age and disease duration did not correlate with Bcl-x(L) expression in rheumatoid patients. Bcl-x(L) may play a role in the extended survival of synoviocytes and inflammatory cells in rheumatoid synovium.en_GB
dc.language.isoengen_GB
dc.subject.meshAgeden_GB
dc.subject.meshAging/metabolismen_GB
dc.subject.meshArthritis, Rheumatoid/*metabolism/pathologyen_GB
dc.subject.meshBlotting, Westernen_GB
dc.subject.meshEndothelial Cells/metabolismen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshImmunohistochemistryen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshOsteoarthritis/metabolism/pathologyen_GB
dc.subject.meshPlasma Cells/metabolismen_GB
dc.subject.meshRNA, Messenger/metabolismen_GB
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_GB
dc.subject.meshStaining and Labelingen_GB
dc.subject.meshSynovial Membrane/*metabolism/pathologyen_GB
dc.subject.meshTime Factorsen_GB
dc.subject.meshbcl-X Protein/genetics/*metabolismen_GB
dc.titleBcl-x(L) expression in vivo in rheumatoid synovium.en_GB
dc.contributor.departmentDepartment of Medicine, Cork University Hospital, National University of Ireland,, Wilton, Cork, Ireland. sandra_busteed@hotmail.comen_GB
dc.identifier.journalClinical rheumatologyen_GB
dc.description.provinceMunster-

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