Hospital-acquired pneumonia after lung resection surgery is associated with characteristic cytokine gene expression.

Hdl Handle:
http://hdl.handle.net/10147/207781
Title:
Hospital-acquired pneumonia after lung resection surgery is associated with characteristic cytokine gene expression.
Authors:
White, Mary; Martin-Loeches, Ignacio; Lawless, Matthew W; O'Dwyer, Michael J; Doherty, Derek G; Young, Vincent; Kelleher, Dermot; McManus, Ross; Ryan, Thomas
Affiliation:
Department of Anaesthesia and Critical Care Medicine, St. James Hospital, and, Deparmtent of Clinical Medicine, Trinity College Dublin, Dublin 8, Ireland., drmbwhite@yahoo.co.uk
Citation:
Chest. 2011 Mar;139(3):626-32. Epub 2010 Sep 9.
Journal:
Chest
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207781
DOI:
10.1378/chest.10-0016
PubMed ID:
20829336
Abstract:
BACKGROUND: Infection in humans has been linked with altered cytokine gene transcription. It is unclear whether this phenomenon is a consequence of an established disease process or precedes the infective process. The primary end point of this study was to determine whether hospital-acquired pneumonia (HAP) was associated with differential gene expression of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and IL-23p19. The secondary end point was to identify whether alteration in gene expression preceded the clinical onset of infection. METHODS: Sixty consecutive patients undergoing elective thoracic surgery were recruited. HAP was diagnosed as per National Nosocomial Infection Surveillance guidelines. Messenger RNA (mRNA) and protein levels were analyzed preoperatively and 24 h and 5 days postoperatively. RESULTS: Forty-one patients had an uncomplicated recovery. Nineteen patients developed HAP. IL-6, IL-10, IL-12p35, IL-23p19, IL-27p28, TNF-alpha, and IFN-gamma mRNA and protein levels of IL-6, IL-23, and IFN-gamma in peripheral blood leukocytes were analyzed before surgery and 24 h and 5 days postsurgery. IL-23p19 mRNA levels were reduced in the pneumonia group (median, 4.19; 10th-90th centile range, 3.90-4.71) compared with the nonpneumonia group (4.50; 3.85-5.32) day 1 postsurgery (P=02). IFN-gamma mRNA levels were reduced in the pneumonia group (2.48; 1.20-3.20) compared with nonpneumonia group (2.81; 2.10-3.26) (P=03) day 5 postsurgery. Results are expressed as log to base 10 copy numbers of cytokine mRNA per 10 million beta-actin mRNA copy numbers. All values are given as median and 10th to 90th centile range. CONCLUSIONS: Cytokine gene expression is altered immediately following surgery in patients with postoperative HAP.
Language:
eng
MeSH:
Aged; Algorithms; Biological Markers/metabolism; Cross Infection/*epidemiology/metabolism; Cytokines/*genetics/metabolism; Disease Susceptibility; Female; Gene Expression Regulation/*physiology; Humans; Interferon-gamma/genetics/metabolism; Interleukin-23/genetics/metabolism; Interleukin-6/genetics/metabolism; Lung/*physiopathology/*surgery; Male; Middle Aged; Pneumonia/*epidemiology/metabolism; Postoperative Complications/*epidemiology/metabolism; Prevalence; Prospective Studies; RNA, Messenger/metabolism; Retrospective Studies; Tumor Necrosis Factor-alpha/genetics/metabolism
ISSN:
1931-3543 (Electronic); 0012-3692 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorWhite, Maryen_GB
dc.contributor.authorMartin-Loeches, Ignacioen_GB
dc.contributor.authorLawless, Matthew Wen_GB
dc.contributor.authorO'Dwyer, Michael Jen_GB
dc.contributor.authorDoherty, Derek Gen_GB
dc.contributor.authorYoung, Vincenten_GB
dc.contributor.authorKelleher, Dermoten_GB
dc.contributor.authorMcManus, Rossen_GB
dc.contributor.authorRyan, Thomasen_GB
dc.date.accessioned2012-02-01T10:44:31Z-
dc.date.available2012-02-01T10:44:31Z-
dc.date.issued2012-02-01T10:44:31Z-
dc.identifier.citationChest. 2011 Mar;139(3):626-32. Epub 2010 Sep 9.en_GB
dc.identifier.issn1931-3543 (Electronic)en_GB
dc.identifier.issn0012-3692 (Linking)en_GB
dc.identifier.pmid20829336en_GB
dc.identifier.doi10.1378/chest.10-0016en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207781-
dc.description.abstractBACKGROUND: Infection in humans has been linked with altered cytokine gene transcription. It is unclear whether this phenomenon is a consequence of an established disease process or precedes the infective process. The primary end point of this study was to determine whether hospital-acquired pneumonia (HAP) was associated with differential gene expression of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and IL-23p19. The secondary end point was to identify whether alteration in gene expression preceded the clinical onset of infection. METHODS: Sixty consecutive patients undergoing elective thoracic surgery were recruited. HAP was diagnosed as per National Nosocomial Infection Surveillance guidelines. Messenger RNA (mRNA) and protein levels were analyzed preoperatively and 24 h and 5 days postoperatively. RESULTS: Forty-one patients had an uncomplicated recovery. Nineteen patients developed HAP. IL-6, IL-10, IL-12p35, IL-23p19, IL-27p28, TNF-alpha, and IFN-gamma mRNA and protein levels of IL-6, IL-23, and IFN-gamma in peripheral blood leukocytes were analyzed before surgery and 24 h and 5 days postsurgery. IL-23p19 mRNA levels were reduced in the pneumonia group (median, 4.19; 10th-90th centile range, 3.90-4.71) compared with the nonpneumonia group (4.50; 3.85-5.32) day 1 postsurgery (P=02). IFN-gamma mRNA levels were reduced in the pneumonia group (2.48; 1.20-3.20) compared with nonpneumonia group (2.81; 2.10-3.26) (P=03) day 5 postsurgery. Results are expressed as log to base 10 copy numbers of cytokine mRNA per 10 million beta-actin mRNA copy numbers. All values are given as median and 10th to 90th centile range. CONCLUSIONS: Cytokine gene expression is altered immediately following surgery in patients with postoperative HAP.en_GB
dc.language.isoengen_GB
dc.subject.meshAgeden_GB
dc.subject.meshAlgorithmsen_GB
dc.subject.meshBiological Markers/metabolismen_GB
dc.subject.meshCross Infection/*epidemiology/metabolismen_GB
dc.subject.meshCytokines/*genetics/metabolismen_GB
dc.subject.meshDisease Susceptibilityen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshGene Expression Regulation/*physiologyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshInterferon-gamma/genetics/metabolismen_GB
dc.subject.meshInterleukin-23/genetics/metabolismen_GB
dc.subject.meshInterleukin-6/genetics/metabolismen_GB
dc.subject.meshLung/*physiopathology/*surgeryen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshPneumonia/*epidemiology/metabolismen_GB
dc.subject.meshPostoperative Complications/*epidemiology/metabolismen_GB
dc.subject.meshPrevalenceen_GB
dc.subject.meshProspective Studiesen_GB
dc.subject.meshRNA, Messenger/metabolismen_GB
dc.subject.meshRetrospective Studiesen_GB
dc.subject.meshTumor Necrosis Factor-alpha/genetics/metabolismen_GB
dc.titleHospital-acquired pneumonia after lung resection surgery is associated with characteristic cytokine gene expression.en_GB
dc.contributor.departmentDepartment of Anaesthesia and Critical Care Medicine, St. James Hospital, and, Deparmtent of Clinical Medicine, Trinity College Dublin, Dublin 8, Ireland., drmbwhite@yahoo.co.uken_GB
dc.identifier.journalChesten_GB
dc.description.provinceLeinster-
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