Desulfovibrio bacterial species are increased in ulcerative colitis.

Hdl Handle:
http://hdl.handle.net/10147/207691
Title:
Desulfovibrio bacterial species are increased in ulcerative colitis.
Authors:
Rowan, Fiachra; Docherty, Neil G; Murphy, Madeline; Murphy, Brendan; Calvin Coffey, John; O'Connell, P Ronan
Affiliation:
Department of Surgery, St. Vincent's University Hospital, UCD School of Medicine , and Medical Sciences, University College Dublin, Dublin, Ireland.
Citation:
Dis Colon Rectum. 2010 Nov;53(11):1530-6.
Journal:
Diseases of the colon and rectum
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207691
DOI:
10.1007/DCR.0b013e3181f1e620
PubMed ID:
20940602
Abstract:
BACKGROUND: Debate persists regarding the role of Desulfovibrio subspecies in ulcerative colitis. Combined microscopic and molecular techniques enable this issue to be investigated by allowing precise enumeration of specific bacterial species within the colonic mucous gel. The aim of this study was to combine laser capture microdissection and quantitative polymerase chain reaction to determine Desulfovibrio copy number in crypt-associated mucous gel in health and in acute and chronic ulcerative colitis. METHODS: Colonic mucosal biopsies were harvested from healthy controls (n = 19) and patients with acute (n = 10) or chronic (n = 10) ulcerative colitis. Crypt-associated mucous gel was obtained by laser capture microdissection throughout the colon. Pan-bacterial 16S rRNA and Desulfovibrio copy number/mm were obtained by polymerase chain reaction at each locus. Bacterial copy numbers were interrogated for correlation with location and disease activity. Data were evaluated using a combination of ordinary linear methods and linear mixed-effects models to cater for multiple interactions. RESULTS: Desulfovibrio positivity was significantly increased in acute and chronic ulcerative colitis at multiple levels within the colon, and after normalization with total bacterial signal, the relative Desulfovibrio load was increased in acute colitis compared with controls. Desulfovibrio counts did not significantly correlate with age, disease duration, or disease activity but interlevel correlations were found in adjacent colonic segments in the healthy control and chronic ulcerative colitis groups. CONCLUSION: The presence of Desulfovibrio subspecies is increased in ulcerative colitis and the data presented suggest that these bacteria represent an increased percentage of the colonic microbiome in acute ulcerative colitis.
Language:
eng
MeSH:
Adolescent; Adult; Aged; Case-Control Studies; Colitis, Ulcerative/*microbiology/pathology; Desulfovibrio/*isolation & purification; Female; Humans; Intestinal Mucosa/microbiology/pathology; Linear Models; Male; Microdissection/methods; Middle Aged; Polymerase Chain Reaction
ISSN:
1530-0358 (Electronic); 0012-3706 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorRowan, Fiachraen_GB
dc.contributor.authorDocherty, Neil Gen_GB
dc.contributor.authorMurphy, Madelineen_GB
dc.contributor.authorMurphy, Brendanen_GB
dc.contributor.authorCalvin Coffey, Johnen_GB
dc.contributor.authorO'Connell, P Ronanen_GB
dc.date.accessioned2012-02-01T10:35:04Z-
dc.date.available2012-02-01T10:35:04Z-
dc.date.issued2012-02-01T10:35:04Z-
dc.identifier.citationDis Colon Rectum. 2010 Nov;53(11):1530-6.en_GB
dc.identifier.issn1530-0358 (Electronic)en_GB
dc.identifier.issn0012-3706 (Linking)en_GB
dc.identifier.pmid20940602en_GB
dc.identifier.doi10.1007/DCR.0b013e3181f1e620en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207691-
dc.description.abstractBACKGROUND: Debate persists regarding the role of Desulfovibrio subspecies in ulcerative colitis. Combined microscopic and molecular techniques enable this issue to be investigated by allowing precise enumeration of specific bacterial species within the colonic mucous gel. The aim of this study was to combine laser capture microdissection and quantitative polymerase chain reaction to determine Desulfovibrio copy number in crypt-associated mucous gel in health and in acute and chronic ulcerative colitis. METHODS: Colonic mucosal biopsies were harvested from healthy controls (n = 19) and patients with acute (n = 10) or chronic (n = 10) ulcerative colitis. Crypt-associated mucous gel was obtained by laser capture microdissection throughout the colon. Pan-bacterial 16S rRNA and Desulfovibrio copy number/mm were obtained by polymerase chain reaction at each locus. Bacterial copy numbers were interrogated for correlation with location and disease activity. Data were evaluated using a combination of ordinary linear methods and linear mixed-effects models to cater for multiple interactions. RESULTS: Desulfovibrio positivity was significantly increased in acute and chronic ulcerative colitis at multiple levels within the colon, and after normalization with total bacterial signal, the relative Desulfovibrio load was increased in acute colitis compared with controls. Desulfovibrio counts did not significantly correlate with age, disease duration, or disease activity but interlevel correlations were found in adjacent colonic segments in the healthy control and chronic ulcerative colitis groups. CONCLUSION: The presence of Desulfovibrio subspecies is increased in ulcerative colitis and the data presented suggest that these bacteria represent an increased percentage of the colonic microbiome in acute ulcerative colitis.en_GB
dc.language.isoengen_GB
dc.subject.meshAdolescenten_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshCase-Control Studiesen_GB
dc.subject.meshColitis, Ulcerative/*microbiology/pathologyen_GB
dc.subject.meshDesulfovibrio/*isolation & purificationen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIntestinal Mucosa/microbiology/pathologyen_GB
dc.subject.meshLinear Modelsen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMicrodissection/methodsen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshPolymerase Chain Reactionen_GB
dc.titleDesulfovibrio bacterial species are increased in ulcerative colitis.en_GB
dc.contributor.departmentDepartment of Surgery, St. Vincent's University Hospital, UCD School of Medicine , and Medical Sciences, University College Dublin, Dublin, Ireland.en_GB
dc.identifier.journalDiseases of the colon and rectumen_GB
dc.description.provinceLeinster-

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