A study of the relationship between albuminuria, proteinuria and urinary reagent strips.

Hdl Handle:
http://hdl.handle.net/10147/207606
Title:
A study of the relationship between albuminuria, proteinuria and urinary reagent strips.
Authors:
Collier, Geraldine; Greenan, Marie Clare; Brady, Jennifer J; Murray, Barbara; Cunningham, Sean K
Affiliation:
Biochemistry Department, St. Vincent's University Hospital, Elm Park, Dublin., gercollier@eircom.net
Citation:
Ann Clin Biochem. 2009 May;46(Pt 3):247-9. Epub 2009 Mar 5.
Journal:
Annals of clinical biochemistry
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207606
DOI:
10.1258/acb.2009.008189
PubMed ID:
19264826
Abstract:
BACKGROUND: The aims of this study were to examine the relationship between proteinuria and albuminuria and to assess the equivalence between the albumin to creatinine ratio (ACR) and the protein to creatinine ratio (PCR) at the cut-offs recommended by the National Institute for Health and Clinical Excellence (NICE) guidance on chronic kidney disease. The sensitivity and specificity of the reagent strips used in our laboratory for the detection of clinical proteinuria was also assessed. METHODS: Urine samples (n = 117) were screened for protein using the Bayer Multistix 10SG and read manually. Urinary total protein and creatinine was measured on the Roche P Modular by the benzethonium chloride and kinetic Jaffe methods, respectively. Urinary albumin was measured by immunoturbidimetry on the Roche Cobas Mira. RESULTS: The relationship between urinary protein and albumin loss was non-linear (P < 0.05). As urinary protein loss increased the percentage of albumin to total protein increased. At the NICE guidance recommended cut-offs for clinical proteinuria (ACR > or =30 mg/mmol and PCR > or =50 mg/mmol) there was one discordant result between ACR and PCR (ACR <30 mg/mmol and PCR >50 mg/mmol). The Bayer Multistix 10SG had a sensitivity and specificity of 97% and 62%, respectively, for the detection of clinical proteinuria compared with ACR. CONCLUSIONS: The proportion of urinary total protein attributable to albumin changes with concentration. There was only one discordant result between ACR and PCR: therefore either ratio may be used for the identification of clinical proteinuria. As a screening test for proteinuria, the Bayer Multistix 10SG had an acceptable sensitivity but poor specificity.
Language:
eng
MeSH:
Albuminuria/*urine; Creatinine/urine; Humans; Proteinuria/*urine; *Reagent Strips; Urinalysis/methods
ISSN:
0004-5632 (Print); 0004-5632 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorCollier, Geraldineen_GB
dc.contributor.authorGreenan, Marie Clareen_GB
dc.contributor.authorBrady, Jennifer Jen_GB
dc.contributor.authorMurray, Barbaraen_GB
dc.contributor.authorCunningham, Sean Ken_GB
dc.date.accessioned2012-02-01T10:32:42Z-
dc.date.available2012-02-01T10:32:42Z-
dc.date.issued2012-02-01T10:32:42Z-
dc.identifier.citationAnn Clin Biochem. 2009 May;46(Pt 3):247-9. Epub 2009 Mar 5.en_GB
dc.identifier.issn0004-5632 (Print)en_GB
dc.identifier.issn0004-5632 (Linking)en_GB
dc.identifier.pmid19264826en_GB
dc.identifier.doi10.1258/acb.2009.008189en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207606-
dc.description.abstractBACKGROUND: The aims of this study were to examine the relationship between proteinuria and albuminuria and to assess the equivalence between the albumin to creatinine ratio (ACR) and the protein to creatinine ratio (PCR) at the cut-offs recommended by the National Institute for Health and Clinical Excellence (NICE) guidance on chronic kidney disease. The sensitivity and specificity of the reagent strips used in our laboratory for the detection of clinical proteinuria was also assessed. METHODS: Urine samples (n = 117) were screened for protein using the Bayer Multistix 10SG and read manually. Urinary total protein and creatinine was measured on the Roche P Modular by the benzethonium chloride and kinetic Jaffe methods, respectively. Urinary albumin was measured by immunoturbidimetry on the Roche Cobas Mira. RESULTS: The relationship between urinary protein and albumin loss was non-linear (P < 0.05). As urinary protein loss increased the percentage of albumin to total protein increased. At the NICE guidance recommended cut-offs for clinical proteinuria (ACR > or =30 mg/mmol and PCR > or =50 mg/mmol) there was one discordant result between ACR and PCR (ACR <30 mg/mmol and PCR >50 mg/mmol). The Bayer Multistix 10SG had a sensitivity and specificity of 97% and 62%, respectively, for the detection of clinical proteinuria compared with ACR. CONCLUSIONS: The proportion of urinary total protein attributable to albumin changes with concentration. There was only one discordant result between ACR and PCR: therefore either ratio may be used for the identification of clinical proteinuria. As a screening test for proteinuria, the Bayer Multistix 10SG had an acceptable sensitivity but poor specificity.en_GB
dc.language.isoengen_GB
dc.subject.meshAlbuminuria/*urineen_GB
dc.subject.meshCreatinine/urineen_GB
dc.subject.meshHumansen_GB
dc.subject.meshProteinuria/*urineen_GB
dc.subject.mesh*Reagent Stripsen_GB
dc.subject.meshUrinalysis/methodsen_GB
dc.titleA study of the relationship between albuminuria, proteinuria and urinary reagent strips.en_GB
dc.contributor.departmentBiochemistry Department, St. Vincent's University Hospital, Elm Park, Dublin., gercollier@eircom.neten_GB
dc.identifier.journalAnnals of clinical biochemistryen_GB
dc.description.provinceLeinster-

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