Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients.

Hdl Handle:
http://hdl.handle.net/10147/207605
Title:
Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients.
Authors:
Kavanagh-Wright, Lucille; Smith, Thomas P; Gibney, James; McKenna, T Joseph
Affiliation:
Department of Endocrinology, St Vincent's University Hospital, Elm Park, Dublin, 4, Ireland. lucille.kavanagh@ucd.i.e
Citation:
Clin Endocrinol (Oxf). 2009 Apr;70(4):599-605. Epub 2008 Sep 2.
Journal:
Clinical endocrinology
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207605
DOI:
10.1111/j.1365-2265.2008.03402.x
PubMed ID:
18771565
Abstract:
OBJECTIVE: It has been reported that macroprolactin is a complex of PRL and an immunoglobulin G (IgG). This study further characterizes macroprolactin and evaluates for other markers of autoimmunity using a cohort of macroprolactinaemic sera. PATIENTS AND NORMAL SUBJECTS: Following treatment of hyperprolactinaemic sera (n = 58) with polyethylene glycol (PEG), PRL values fell from 524-13 546 mU/l (Range) to 452-8455 mU/l, while in macroprolactinaemic sera (n = 41), PRL concentration fell from 525-5747 to 98-378 mU/l (PEG treated normoprolactinaemic reference range, 68-230 mU/l in males, 70-390 mU/l in females). DESIGN: PRL was measured in sera prior to and following gel filtration chromatography, ultrafiltration, treatment with protein A-sepharose, protein G-sepharose, antihuman IgG-agarose and sodium thiocyanate (NaSCN). The binding of radio-labelled PRL in macroprolactinaemic sera was also measured. Sera were assayed for antithyroid and antinuclear antibodies. C-reactive protein (CRP) and CD5 positive B cells were also measured. Comparisons were made between values obtained in normal, hyperprolactinaemic and macroprolactinaemic sera. Results Macroprolactinaemic sera indicated the presence of an IgG molecule and/or IgG fragments with one or more molecules of PRL. In 97% of the sera macroprolactin had a molecular weight of 204 kDa. Treatment of macroprolactinaemic sera with NaSCN caused dissociation of macroprolactin, releasing monomeric PRL. Macroprolactinaemic sera did not yield evidence of an increase in markers of autoimmunity when compared with hyperprolactinaemic or normal sera. CONCLUSIONS: Comprehensive analysis of macroprolactin confirmed its composition as an IgG molecule or fragment with a PRL molecule. The occurrence of macroprolactin does not appear to be associated with autoimmunity.
Language:
eng
MeSH:
Autoimmunity/*physiology; Biological Markers/blood; Case-Control Studies; Chromatography, Agarose; Chromatography, Gel; Female; Humans; Hyperprolactinemia/*blood/immunology; Immunoglobulin G/*blood; Immunoprecipitation; Male; Prolactin/*blood; Ultrafiltration
ISSN:
1365-2265 (Electronic); 0300-0664 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorKavanagh-Wright, Lucilleen_GB
dc.contributor.authorSmith, Thomas Pen_GB
dc.contributor.authorGibney, Jamesen_GB
dc.contributor.authorMcKenna, T Josephen_GB
dc.date.accessioned2012-02-01T10:32:40Z-
dc.date.available2012-02-01T10:32:40Z-
dc.date.issued2012-02-01T10:32:40Z-
dc.identifier.citationClin Endocrinol (Oxf). 2009 Apr;70(4):599-605. Epub 2008 Sep 2.en_GB
dc.identifier.issn1365-2265 (Electronic)en_GB
dc.identifier.issn0300-0664 (Linking)en_GB
dc.identifier.pmid18771565en_GB
dc.identifier.doi10.1111/j.1365-2265.2008.03402.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/207605-
dc.description.abstractOBJECTIVE: It has been reported that macroprolactin is a complex of PRL and an immunoglobulin G (IgG). This study further characterizes macroprolactin and evaluates for other markers of autoimmunity using a cohort of macroprolactinaemic sera. PATIENTS AND NORMAL SUBJECTS: Following treatment of hyperprolactinaemic sera (n = 58) with polyethylene glycol (PEG), PRL values fell from 524-13 546 mU/l (Range) to 452-8455 mU/l, while in macroprolactinaemic sera (n = 41), PRL concentration fell from 525-5747 to 98-378 mU/l (PEG treated normoprolactinaemic reference range, 68-230 mU/l in males, 70-390 mU/l in females). DESIGN: PRL was measured in sera prior to and following gel filtration chromatography, ultrafiltration, treatment with protein A-sepharose, protein G-sepharose, antihuman IgG-agarose and sodium thiocyanate (NaSCN). The binding of radio-labelled PRL in macroprolactinaemic sera was also measured. Sera were assayed for antithyroid and antinuclear antibodies. C-reactive protein (CRP) and CD5 positive B cells were also measured. Comparisons were made between values obtained in normal, hyperprolactinaemic and macroprolactinaemic sera. Results Macroprolactinaemic sera indicated the presence of an IgG molecule and/or IgG fragments with one or more molecules of PRL. In 97% of the sera macroprolactin had a molecular weight of 204 kDa. Treatment of macroprolactinaemic sera with NaSCN caused dissociation of macroprolactin, releasing monomeric PRL. Macroprolactinaemic sera did not yield evidence of an increase in markers of autoimmunity when compared with hyperprolactinaemic or normal sera. CONCLUSIONS: Comprehensive analysis of macroprolactin confirmed its composition as an IgG molecule or fragment with a PRL molecule. The occurrence of macroprolactin does not appear to be associated with autoimmunity.en_GB
dc.language.isoengen_GB
dc.subject.meshAutoimmunity/*physiologyen_GB
dc.subject.meshBiological Markers/blooden_GB
dc.subject.meshCase-Control Studiesen_GB
dc.subject.meshChromatography, Agaroseen_GB
dc.subject.meshChromatography, Gelen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshHyperprolactinemia/*blood/immunologyen_GB
dc.subject.meshImmunoglobulin G/*blooden_GB
dc.subject.meshImmunoprecipitationen_GB
dc.subject.meshMaleen_GB
dc.subject.meshProlactin/*blooden_GB
dc.subject.meshUltrafiltrationen_GB
dc.titleCharacterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients.en_GB
dc.contributor.departmentDepartment of Endocrinology, St Vincent's University Hospital, Elm Park, Dublin, 4, Ireland. lucille.kavanagh@ucd.i.een_GB
dc.identifier.journalClinical endocrinologyen_GB
dc.description.provinceLeinster-
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