High clusterin expression correlates with a poor outcome in stage II colorectal cancers.

Hdl Handle:
http://hdl.handle.net/10147/207569
Title:
High clusterin expression correlates with a poor outcome in stage II colorectal cancers.
Authors:
Kevans, David; Foley, Jane; Tenniswood, Martin; Sheahan, Kieran; Hyland, John; O'Donoghue, Diarmuid; Mulcahy, Hugh; O'Sullivan, Jacintha
Affiliation:
Centre for Colorectal Disease, St Vincent's University Hospital, Elm Park, Dublin, 4, Ireland.
Citation:
Cancer Epidemiol Biomarkers Prev. 2009 Feb;18(2):393-9. Epub 2009 Jan 20.
Journal:
Cancer epidemiology, biomarkers & prevention : a publication of the American, Association for Cancer Research, cosponsored by the American Society of, Preventive Oncology
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207569
DOI:
10.1158/1055-9965.EPI-08-0302
PubMed ID:
19155441
Abstract:
The role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. The study included 251 patients with stage II colorectal cancer. Tissue microarrays were constructed and immunohistochemistry done and correlated with clinical features and long term outcome. Dual immunofluorescence and confocal microscopy were used with terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling probes and clusterin antibody to assess the degree of co localization. Percentage epithelial cytoplasmic staining was higher in tumor compared with nonadjacent normal mucosa (P < 0.001). Within the stromal compartment, percentage cytoplamic staining and intensity was lower in tumor tissue compared with normal nonadjacent mucosa (P < or = 0.001). Survival was significantly associated with percentage epithelial cytoplasmic staining (P < 0.001), epithelial cytoplasmic staining intensity (P < 0.001), percentage stromal cytoplasmic staining (P = 0.002), and stromal cytoplasmic staining intensity (P < 0.001). Clusterin levels are associated with poor survival in stage II colorectal cancer.
Language:
eng
MeSH:
Adult; Aged; Aged, 80 and over; Chi-Square Distribution; Clusterin/*metabolism; Colorectal Neoplasms/*metabolism/pathology; Female; Humans; Immunoenzyme Techniques; In Situ Nick-End Labeling; Male; Microarray Analysis; Middle Aged; Neoplasm Staging; Prognosis; Reproducibility of Results; Statistics, Nonparametric; Tumor Markers, Biological/*metabolism
ISSN:
1055-9965 (Print); 1055-9965 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorKevans, Daviden_GB
dc.contributor.authorFoley, Janeen_GB
dc.contributor.authorTenniswood, Martinen_GB
dc.contributor.authorSheahan, Kieranen_GB
dc.contributor.authorHyland, Johnen_GB
dc.contributor.authorO'Donoghue, Diarmuiden_GB
dc.contributor.authorMulcahy, Hughen_GB
dc.contributor.authorO'Sullivan, Jacinthaen_GB
dc.date.accessioned2012-02-01T10:31:38Z-
dc.date.available2012-02-01T10:31:38Z-
dc.date.issued2012-02-01T10:31:38Z-
dc.identifier.citationCancer Epidemiol Biomarkers Prev. 2009 Feb;18(2):393-9. Epub 2009 Jan 20.en_GB
dc.identifier.issn1055-9965 (Print)en_GB
dc.identifier.issn1055-9965 (Linking)en_GB
dc.identifier.pmid19155441en_GB
dc.identifier.doi10.1158/1055-9965.EPI-08-0302en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207569-
dc.description.abstractThe role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. The study included 251 patients with stage II colorectal cancer. Tissue microarrays were constructed and immunohistochemistry done and correlated with clinical features and long term outcome. Dual immunofluorescence and confocal microscopy were used with terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling probes and clusterin antibody to assess the degree of co localization. Percentage epithelial cytoplasmic staining was higher in tumor compared with nonadjacent normal mucosa (P < 0.001). Within the stromal compartment, percentage cytoplamic staining and intensity was lower in tumor tissue compared with normal nonadjacent mucosa (P < or = 0.001). Survival was significantly associated with percentage epithelial cytoplasmic staining (P < 0.001), epithelial cytoplasmic staining intensity (P < 0.001), percentage stromal cytoplasmic staining (P = 0.002), and stromal cytoplasmic staining intensity (P < 0.001). Clusterin levels are associated with poor survival in stage II colorectal cancer.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshAged, 80 and overen_GB
dc.subject.meshChi-Square Distributionen_GB
dc.subject.meshClusterin/*metabolismen_GB
dc.subject.meshColorectal Neoplasms/*metabolism/pathologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshImmunoenzyme Techniquesen_GB
dc.subject.meshIn Situ Nick-End Labelingen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMicroarray Analysisen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshNeoplasm Stagingen_GB
dc.subject.meshPrognosisen_GB
dc.subject.meshReproducibility of Resultsen_GB
dc.subject.meshStatistics, Nonparametricen_GB
dc.subject.meshTumor Markers, Biological/*metabolismen_GB
dc.titleHigh clusterin expression correlates with a poor outcome in stage II colorectal cancers.en_GB
dc.contributor.departmentCentre for Colorectal Disease, St Vincent's University Hospital, Elm Park, Dublin, 4, Ireland.en_GB
dc.identifier.journalCancer epidemiology, biomarkers & prevention : a publication of the American, Association for Cancer Research, cosponsored by the American Society of, Preventive Oncologyen_GB
dc.description.provinceLeinster-

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