Levels of oxidative damage and lipid peroxidation in thyroid neoplasia.
Affiliation
Department of Otolaryngology, Head & Neck Surgery, UCD School of Medicine &, Medical Science, St Vincent's University Hospital, Elm Park, Dublin.Issue Date
2012-02-01T10:30:30ZMeSH
Adenoma/*physiopathologyAdolescent
Adult
Aged
Aldehydes/metabolism
Antibodies, Monoclonal
Cytoplasm/metabolism
*DNA Damage/physiology
Deoxyguanosine/analogs & derivatives/metabolism
Female
Humans
*Lipid Peroxidation
Male
Middle Aged
Oxidative Stress/physiology
Protein Array Analysis
Thyroid Neoplasms/*physiopathology
Young Adult
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Head Neck. 2010 Jun;32(6):750-6.Journal
Head & neckDOI
10.1002/hed.21247PubMed ID
19998441Abstract
BACKGROUND: This study assessed the presence of oxidative damage and lipid peroxidation in thyroid neoplasia. METHODS: Using tissue microarrays and immunohistochemistry, we assessed levels of DNA damage (8-oxo-dG) and lipid peroxidation (4-HNE) in 71 follicular thyroid adenoma (FTA), 45 papillary thyroid carcinoma (PTC), and 17 follicular thyroid carcinoma (FTC) and matched normal thyroid tissue. RESULTS: Cytoplasmic 8-oxo-dG and 4-HNE expression was significantly higher in FTA, FTC, and PTC tissue compared to matched normal tissue (all p values < .001). Similarly, elevated nuclear levels of 8-oxo-dG were seen in all in FTA, FTC, and PTC tissue compared to matched normal (p values < .07, < .001, < .001, respectively). In contrast, a higher level of 4-HNE expression was detected in normal thyroid tissue compared with matched tumor tissue (p < .001 for all groups). Comparing all 3 groups, 4-HNE levels were higher than 8-oxo-dG levels (p < .001 for all groups) except that cytoplasmic levels of 8-oxo-dG were higher than 4-HNE in all (p < .001). These results were independent of proliferation status. CONCLUSION: High levels of DNA damage and lipid peroxidation in benign and malignant thyroid neoplasia indicates this damage is an early event that may influence disease progression.Language
engISSN
1097-0347 (Electronic)1043-3074 (Linking)
ae974a485f413a2113503eed53cd6c53
10.1002/hed.21247
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