Levels of oxidative damage and lipid peroxidation in thyroid neoplasia.

Hdl Handle:
http://hdl.handle.net/10147/207532
Title:
Levels of oxidative damage and lipid peroxidation in thyroid neoplasia.
Authors:
Young, Orla; Crotty, Tom; O'Connell, Rohana; O'Sullivan, Jacintha; Curran, Aongus J
Affiliation:
Department of Otolaryngology, Head & Neck Surgery, UCD School of Medicine &, Medical Science, St Vincent's University Hospital, Elm Park, Dublin.
Citation:
Head Neck. 2010 Jun;32(6):750-6.
Journal:
Head & neck
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207532
DOI:
10.1002/hed.21247
PubMed ID:
19998441
Abstract:
BACKGROUND: This study assessed the presence of oxidative damage and lipid peroxidation in thyroid neoplasia. METHODS: Using tissue microarrays and immunohistochemistry, we assessed levels of DNA damage (8-oxo-dG) and lipid peroxidation (4-HNE) in 71 follicular thyroid adenoma (FTA), 45 papillary thyroid carcinoma (PTC), and 17 follicular thyroid carcinoma (FTC) and matched normal thyroid tissue. RESULTS: Cytoplasmic 8-oxo-dG and 4-HNE expression was significantly higher in FTA, FTC, and PTC tissue compared to matched normal tissue (all p values < .001). Similarly, elevated nuclear levels of 8-oxo-dG were seen in all in FTA, FTC, and PTC tissue compared to matched normal (p values < .07, < .001, < .001, respectively). In contrast, a higher level of 4-HNE expression was detected in normal thyroid tissue compared with matched tumor tissue (p < .001 for all groups). Comparing all 3 groups, 4-HNE levels were higher than 8-oxo-dG levels (p < .001 for all groups) except that cytoplasmic levels of 8-oxo-dG were higher than 4-HNE in all (p < .001). These results were independent of proliferation status. CONCLUSION: High levels of DNA damage and lipid peroxidation in benign and malignant thyroid neoplasia indicates this damage is an early event that may influence disease progression.
Language:
eng
MeSH:
Adenoma/*physiopathology; Adolescent; Adult; Aged; Aldehydes/metabolism; Antibodies, Monoclonal; Cytoplasm/metabolism; *DNA Damage/physiology; Deoxyguanosine/analogs & derivatives/metabolism; Female; Humans; *Lipid Peroxidation; Male; Middle Aged; Oxidative Stress/physiology; Protein Array Analysis; Thyroid Neoplasms/*physiopathology; Young Adult
ISSN:
1097-0347 (Electronic); 1043-3074 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorYoung, Orlaen_GB
dc.contributor.authorCrotty, Tomen_GB
dc.contributor.authorO'Connell, Rohanaen_GB
dc.contributor.authorO'Sullivan, Jacinthaen_GB
dc.contributor.authorCurran, Aongus Jen_GB
dc.date.accessioned2012-02-01T10:30:30Z-
dc.date.available2012-02-01T10:30:30Z-
dc.date.issued2012-02-01T10:30:30Z-
dc.identifier.citationHead Neck. 2010 Jun;32(6):750-6.en_GB
dc.identifier.issn1097-0347 (Electronic)en_GB
dc.identifier.issn1043-3074 (Linking)en_GB
dc.identifier.pmid19998441en_GB
dc.identifier.doi10.1002/hed.21247en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207532-
dc.description.abstractBACKGROUND: This study assessed the presence of oxidative damage and lipid peroxidation in thyroid neoplasia. METHODS: Using tissue microarrays and immunohistochemistry, we assessed levels of DNA damage (8-oxo-dG) and lipid peroxidation (4-HNE) in 71 follicular thyroid adenoma (FTA), 45 papillary thyroid carcinoma (PTC), and 17 follicular thyroid carcinoma (FTC) and matched normal thyroid tissue. RESULTS: Cytoplasmic 8-oxo-dG and 4-HNE expression was significantly higher in FTA, FTC, and PTC tissue compared to matched normal tissue (all p values < .001). Similarly, elevated nuclear levels of 8-oxo-dG were seen in all in FTA, FTC, and PTC tissue compared to matched normal (p values < .07, < .001, < .001, respectively). In contrast, a higher level of 4-HNE expression was detected in normal thyroid tissue compared with matched tumor tissue (p < .001 for all groups). Comparing all 3 groups, 4-HNE levels were higher than 8-oxo-dG levels (p < .001 for all groups) except that cytoplasmic levels of 8-oxo-dG were higher than 4-HNE in all (p < .001). These results were independent of proliferation status. CONCLUSION: High levels of DNA damage and lipid peroxidation in benign and malignant thyroid neoplasia indicates this damage is an early event that may influence disease progression.en_GB
dc.language.isoengen_GB
dc.subject.meshAdenoma/*physiopathologyen_GB
dc.subject.meshAdolescenten_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshAldehydes/metabolismen_GB
dc.subject.meshAntibodies, Monoclonalen_GB
dc.subject.meshCytoplasm/metabolismen_GB
dc.subject.mesh*DNA Damage/physiologyen_GB
dc.subject.meshDeoxyguanosine/analogs & derivatives/metabolismen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.mesh*Lipid Peroxidationen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshOxidative Stress/physiologyen_GB
dc.subject.meshProtein Array Analysisen_GB
dc.subject.meshThyroid Neoplasms/*physiopathologyen_GB
dc.subject.meshYoung Adulten_GB
dc.titleLevels of oxidative damage and lipid peroxidation in thyroid neoplasia.en_GB
dc.contributor.departmentDepartment of Otolaryngology, Head & Neck Surgery, UCD School of Medicine &, Medical Science, St Vincent's University Hospital, Elm Park, Dublin.en_GB
dc.identifier.journalHead & necken_GB
dc.description.provinceLeinster-

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