Coeliac disease autoantibodies mediate significant inhibition of tissue transglutaminase.

Hdl Handle:
http://hdl.handle.net/10147/207452
Title:
Coeliac disease autoantibodies mediate significant inhibition of tissue transglutaminase.
Authors:
Byrne, Greg; Feighery, Con; Jackson, John; Kelly, Jacinta
Affiliation:
National Children's Research Centre, Our Lady's Children's Hospital, Crumlin,, Dublin 12, Ireland. greg.byrne@ucd.ie
Citation:
Clin Immunol. 2010 Sep;136(3):426-31. Epub 2010 May 21.
Journal:
Clinical immunology (Orlando, Fla.)
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207452
DOI:
10.1016/j.clim.2010.04.017
PubMed ID:
20488756
Abstract:
The detection of antibodies directed against tissue transglutaminase (tTG) in serum is a sensitive and specific test for suspected coeliac disease. tTG is a ubiquitous, multifunctional enzyme that has been implicated in many important physiological processes as well as the site-specific deamidation of glutamine residues in gluten-derived peptides. This modification of gluten peptides facilitates their binding to HLA-DQ2, which results in amplification of the T-cell response to gluten. The purpose of this study was to investigate the possibility that patient IgA autoantibodies directed against tTG interfere with the crosslinking activity of the enzyme. IgA autoantibodies against tTG were isolated/depleted from patient serum and tested for their capacity to interfere with tTG activity in vitro using a sensitive fluorescence-based activity assay. We have demonstrated that autoantibodies cause significant inhibition of tTG-mediated crosslinking at equimolar and 2:1 ratios of antibody to enzyme.
Language:
eng
MeSH:
Adult; Aged; Aged, 80 and over; Autoantibodies/*blood; Celiac Disease/*enzymology/*immunology; Female; Glutens/immunology/metabolism; HLA-DQ Antigens/metabolism; Humans; Immunoglobulin A/blood; Male; Middle Aged; Recombinant Proteins/antagonists & inhibitors/immunology/metabolism; T-Lymphocytes/immunology; Transglutaminases/*antagonists & inhibitors/*immunology/metabolism; Young Adult
ISSN:
1521-7035 (Electronic); 1521-6616 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorByrne, Gregen_GB
dc.contributor.authorFeighery, Conen_GB
dc.contributor.authorJackson, Johnen_GB
dc.contributor.authorKelly, Jacintaen_GB
dc.date.accessioned2012-02-01T10:25:22Z-
dc.date.available2012-02-01T10:25:22Z-
dc.date.issued2012-02-01T10:25:22Z-
dc.identifier.citationClin Immunol. 2010 Sep;136(3):426-31. Epub 2010 May 21.en_GB
dc.identifier.issn1521-7035 (Electronic)en_GB
dc.identifier.issn1521-6616 (Linking)en_GB
dc.identifier.pmid20488756en_GB
dc.identifier.doi10.1016/j.clim.2010.04.017en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207452-
dc.description.abstractThe detection of antibodies directed against tissue transglutaminase (tTG) in serum is a sensitive and specific test for suspected coeliac disease. tTG is a ubiquitous, multifunctional enzyme that has been implicated in many important physiological processes as well as the site-specific deamidation of glutamine residues in gluten-derived peptides. This modification of gluten peptides facilitates their binding to HLA-DQ2, which results in amplification of the T-cell response to gluten. The purpose of this study was to investigate the possibility that patient IgA autoantibodies directed against tTG interfere with the crosslinking activity of the enzyme. IgA autoantibodies against tTG were isolated/depleted from patient serum and tested for their capacity to interfere with tTG activity in vitro using a sensitive fluorescence-based activity assay. We have demonstrated that autoantibodies cause significant inhibition of tTG-mediated crosslinking at equimolar and 2:1 ratios of antibody to enzyme.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshAged, 80 and overen_GB
dc.subject.meshAutoantibodies/*blooden_GB
dc.subject.meshCeliac Disease/*enzymology/*immunologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshGlutens/immunology/metabolismen_GB
dc.subject.meshHLA-DQ Antigens/metabolismen_GB
dc.subject.meshHumansen_GB
dc.subject.meshImmunoglobulin A/blooden_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshRecombinant Proteins/antagonists & inhibitors/immunology/metabolismen_GB
dc.subject.meshT-Lymphocytes/immunologyen_GB
dc.subject.meshTransglutaminases/*antagonists & inhibitors/*immunology/metabolismen_GB
dc.subject.meshYoung Adulten_GB
dc.titleCoeliac disease autoantibodies mediate significant inhibition of tissue transglutaminase.en_GB
dc.contributor.departmentNational Children's Research Centre, Our Lady's Children's Hospital, Crumlin,, Dublin 12, Ireland. greg.byrne@ucd.ieen_GB
dc.identifier.journalClinical immunology (Orlando, Fla.)en_GB
dc.description.provinceLeinster-

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