Campylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.

Hdl Handle:
http://hdl.handle.net/10147/207412
Title:
Campylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.
Authors:
Corcionivoschi, N; Clyne, M; Lyons, A; Elmi, A; Gundogdu, O; Wren, B W; Dorrell, N; Karlyshev, A V; Bourke, B
Affiliation:
The Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, 12, Ireland.
Citation:
Infect Immun. 2009 May;77(5):1959-67. Epub 2009 Mar 9.
Journal:
Infection and immunity
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207412
DOI:
10.1128/IAI.01239-08
PubMed ID:
19273563
Abstract:
The host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar depletion occurred only when the bacterial and epithelial cells were cocultured. CPS depletion occurred when C. jejuni organisms were exposed to conditioned media from a different C. jejuni strain but not when exposed to conditioned media from other bacterial species. Proteinase K or heat treatment of conditioned media under coculture conditions abrogated the effect on the sugars, as did formaldehyde fixation and cycloheximide treatment of host cells or chloramphenicol treatment of the bacteria. However, sugar depletion was not affected in flagellar export (fliQ) and quorum-sensing (luxS) gene mutants. Passaged C. jejuni showed reduced invasiveness and increased serum sensitivity in vitro. C. jejuni alters its surface polysaccharides when cocultured with epithelial cells, suggesting the existence of a cross talk mechanism that modulates CPS expression during infection.
Language:
eng
MeSH:
Campylobacter jejuni/*physiology; Cell Line; Coculture Techniques; Culture Media, Conditioned; Epithelial Cells/*microbiology; Gene Expression Profiling; Humans; Oligonucleotide Array Sequence Analysis; Polysaccharides, Bacterial/*biosynthesis
ISSN:
1098-5522 (Electronic); 0019-9567 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorCorcionivoschi, Nen_GB
dc.contributor.authorClyne, Men_GB
dc.contributor.authorLyons, Aen_GB
dc.contributor.authorElmi, Aen_GB
dc.contributor.authorGundogdu, Oen_GB
dc.contributor.authorWren, B Wen_GB
dc.contributor.authorDorrell, Nen_GB
dc.contributor.authorKarlyshev, A Ven_GB
dc.contributor.authorBourke, Ben_GB
dc.date.accessioned2012-02-01T10:24:21Z-
dc.date.available2012-02-01T10:24:21Z-
dc.date.issued2012-02-01T10:24:21Z-
dc.identifier.citationInfect Immun. 2009 May;77(5):1959-67. Epub 2009 Mar 9.en_GB
dc.identifier.issn1098-5522 (Electronic)en_GB
dc.identifier.issn0019-9567 (Linking)en_GB
dc.identifier.pmid19273563en_GB
dc.identifier.doi10.1128/IAI.01239-08en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207412-
dc.description.abstractThe host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar depletion occurred only when the bacterial and epithelial cells were cocultured. CPS depletion occurred when C. jejuni organisms were exposed to conditioned media from a different C. jejuni strain but not when exposed to conditioned media from other bacterial species. Proteinase K or heat treatment of conditioned media under coculture conditions abrogated the effect on the sugars, as did formaldehyde fixation and cycloheximide treatment of host cells or chloramphenicol treatment of the bacteria. However, sugar depletion was not affected in flagellar export (fliQ) and quorum-sensing (luxS) gene mutants. Passaged C. jejuni showed reduced invasiveness and increased serum sensitivity in vitro. C. jejuni alters its surface polysaccharides when cocultured with epithelial cells, suggesting the existence of a cross talk mechanism that modulates CPS expression during infection.en_GB
dc.language.isoengen_GB
dc.subject.meshCampylobacter jejuni/*physiologyen_GB
dc.subject.meshCell Lineen_GB
dc.subject.meshCoculture Techniquesen_GB
dc.subject.meshCulture Media, Conditioneden_GB
dc.subject.meshEpithelial Cells/*microbiologyen_GB
dc.subject.meshGene Expression Profilingen_GB
dc.subject.meshHumansen_GB
dc.subject.meshOligonucleotide Array Sequence Analysisen_GB
dc.subject.meshPolysaccharides, Bacterial/*biosynthesisen_GB
dc.titleCampylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.en_GB
dc.contributor.departmentThe Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, 12, Ireland.en_GB
dc.identifier.journalInfection and immunityen_GB
dc.description.provinceLeinster-

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