Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.

Hdl Handle:
http://hdl.handle.net/10147/207397
Title:
Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.
Authors:
Logan, Catriona; Habington, Adele; Lennon, Grainne; Cronin, Frank; O'Sullivan, Niamh
Affiliation:
Department of Microbiology, Our Lady's Children's Hospital, Crumlin, Dublin,, Ireland. catrionalogan@eircom.net
Citation:
Diagn Microbiol Infect Dis. 2010 Dec;68(4):358-65. Epub 2010 Sep 29.
Journal:
Diagnostic microbiology and infectious disease
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207397
DOI:
10.1016/j.diagmicrobio.2010.07.012
PubMed ID:
20884156
Abstract:
A longitudinal study of 2099 sputa and throat swabs received from 183 pediatric cystic fibrosis patients over a 29-month period was used to evaluate the efficacy of real-time polymerase chain reaction (PCR) for the early detection of Pseudomonas aeruginosa as compared to microbiologic culture. Real-time PCR resulted in an increased number of specimens identified as P. aeruginosa positive. The sensitivity of culture was 82% (373/453) and of PCR was 93% (420/453) when considering both positive culture and PCR results as true positives. Of the 80 specimens identified as PCR positive/culture negative for P. aeruginosa, the subsequent patient sample in 32.5% (26/80) of specimens concerned was identified as P. aeruginosa culture positive, suggesting that PCR has the potential to detect P. aeruginosa earlier than the microbiologic culture. Real-time PCR analysis found no evidence of the Liverpool and Manchester epidemic P. aeruginosa strains in the cohort examined. The findings of this study highlight the importance of specimen collection protocols to ensure that adequate samples are received at the laboratory for testing, thereby minimizing the potential for reporting of false-negative P. aeruginosa culture results.
Language:
eng
MeSH:
Adolescent; Bacteriological Techniques; Child; Child, Preschool; Culture Media; Cystic Fibrosis/*microbiology; DNA, Bacterial/analysis/isolation & purification; Early Diagnosis; Humans; Infant; Infant, Newborn; Pharynx/*microbiology; Polymerase Chain Reaction/*methods; Pseudomonas Infections/*diagnosis/microbiology; Pseudomonas aeruginosa/genetics/*isolation & purification; Sensitivity and Specificity; Specimen Handling/methods; Sputum/*microbiology; Young Adult
ISSN:
1879-0070 (Electronic); 0732-8893 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorLogan, Catrionaen_GB
dc.contributor.authorHabington, Adeleen_GB
dc.contributor.authorLennon, Grainneen_GB
dc.contributor.authorCronin, Franken_GB
dc.contributor.authorO'Sullivan, Niamhen_GB
dc.date.accessioned2012-02-01T10:23:57Z-
dc.date.available2012-02-01T10:23:57Z-
dc.date.issued2012-02-01T10:23:57Z-
dc.identifier.citationDiagn Microbiol Infect Dis. 2010 Dec;68(4):358-65. Epub 2010 Sep 29.en_GB
dc.identifier.issn1879-0070 (Electronic)en_GB
dc.identifier.issn0732-8893 (Linking)en_GB
dc.identifier.pmid20884156en_GB
dc.identifier.doi10.1016/j.diagmicrobio.2010.07.012en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207397-
dc.description.abstractA longitudinal study of 2099 sputa and throat swabs received from 183 pediatric cystic fibrosis patients over a 29-month period was used to evaluate the efficacy of real-time polymerase chain reaction (PCR) for the early detection of Pseudomonas aeruginosa as compared to microbiologic culture. Real-time PCR resulted in an increased number of specimens identified as P. aeruginosa positive. The sensitivity of culture was 82% (373/453) and of PCR was 93% (420/453) when considering both positive culture and PCR results as true positives. Of the 80 specimens identified as PCR positive/culture negative for P. aeruginosa, the subsequent patient sample in 32.5% (26/80) of specimens concerned was identified as P. aeruginosa culture positive, suggesting that PCR has the potential to detect P. aeruginosa earlier than the microbiologic culture. Real-time PCR analysis found no evidence of the Liverpool and Manchester epidemic P. aeruginosa strains in the cohort examined. The findings of this study highlight the importance of specimen collection protocols to ensure that adequate samples are received at the laboratory for testing, thereby minimizing the potential for reporting of false-negative P. aeruginosa culture results.en_GB
dc.language.isoengen_GB
dc.subject.meshAdolescenten_GB
dc.subject.meshBacteriological Techniquesen_GB
dc.subject.meshChilden_GB
dc.subject.meshChild, Preschoolen_GB
dc.subject.meshCulture Mediaen_GB
dc.subject.meshCystic Fibrosis/*microbiologyen_GB
dc.subject.meshDNA, Bacterial/analysis/isolation & purificationen_GB
dc.subject.meshEarly Diagnosisen_GB
dc.subject.meshHumansen_GB
dc.subject.meshInfanten_GB
dc.subject.meshInfant, Newbornen_GB
dc.subject.meshPharynx/*microbiologyen_GB
dc.subject.meshPolymerase Chain Reaction/*methodsen_GB
dc.subject.meshPseudomonas Infections/*diagnosis/microbiologyen_GB
dc.subject.meshPseudomonas aeruginosa/genetics/*isolation & purificationen_GB
dc.subject.meshSensitivity and Specificityen_GB
dc.subject.meshSpecimen Handling/methodsen_GB
dc.subject.meshSputum/*microbiologyen_GB
dc.subject.meshYoung Adulten_GB
dc.titleEvaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.en_GB
dc.contributor.departmentDepartment of Microbiology, Our Lady's Children's Hospital, Crumlin, Dublin,, Ireland. catrionalogan@eircom.neten_GB
dc.identifier.journalDiagnostic microbiology and infectious diseaseen_GB
dc.description.provinceLeinster-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.