Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model.

Hdl Handle:
http://hdl.handle.net/10147/207210
Title:
Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model.
Authors:
O'Sullivan, J B; Hanson, R; Chan, F; Bouchier-Hayes, D J
Affiliation:
Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital,, Dublin, Ireland. jbarryosullivan@mac.com
Citation:
Ir J Med Sci. 2011 Mar;180(1):229-36. Epub 2010 Nov 26.
Journal:
Irish journal of medical science
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207210
DOI:
10.1007/s11845-010-0630-z
PubMed ID:
21110137
Abstract:
BACKGROUND: Diabetes mellitus is a leading cause of impaired wound healing. The aim of this study was to establish a glucose-controlled diabetic wound healing model. METHOD: Sprague-Dawley rats were divided into three groups: Control group (C), Diabetic Non-glucose Controlled group (DNC) and Diabetic glucose Controlled group (DC). RESULTS: Glucose control was achieved using Insulman Rapid (average daily glucose level <10 mmol/L). 18 Sprague-Dawley rats underwent a dorsal skin wound incision and 10 days later were killed. Fresh and fixed wound tensile strength, hydroxyproline and transforming growth factor beta-1 levels were improved in the DC group when compared to the DNC group. The quantity of fibroblasts present was similar in each group. CONCLUSION: This study demonstrates the impact that diabetes has on acute wound healing and suggests that wound modulating agents must be tested in both the tightly glucose-controlled as well as the poorly glucose-controlled diabetic animal models prior to proceeding with translational clinical studies.
Language:
eng
MeSH:
Animals; Blood Glucose/*analysis; Body Fluids/chemistry; Diabetes Mellitus, Experimental/blood/*physiopathology; Fibroblasts/metabolism; Hydroxyproline/analysis; Immunohistochemistry; Male; Models, Animal; Rats; Rats, Sprague-Dawley; Tensile Strength; Transforming Growth Factor beta1/analysis; Wound Healing/*physiology
ISSN:
1863-4362 (Electronic); 0021-1265 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Sullivan, J Ben_GB
dc.contributor.authorHanson, Ren_GB
dc.contributor.authorChan, Fen_GB
dc.contributor.authorBouchier-Hayes, D Jen_GB
dc.date.accessioned2012-02-01T10:02:02Z-
dc.date.available2012-02-01T10:02:02Z-
dc.date.issued2012-02-01T10:02:02Z-
dc.identifier.citationIr J Med Sci. 2011 Mar;180(1):229-36. Epub 2010 Nov 26.en_GB
dc.identifier.issn1863-4362 (Electronic)en_GB
dc.identifier.issn0021-1265 (Linking)en_GB
dc.identifier.pmid21110137en_GB
dc.identifier.doi10.1007/s11845-010-0630-zen_GB
dc.identifier.urihttp://hdl.handle.net/10147/207210-
dc.description.abstractBACKGROUND: Diabetes mellitus is a leading cause of impaired wound healing. The aim of this study was to establish a glucose-controlled diabetic wound healing model. METHOD: Sprague-Dawley rats were divided into three groups: Control group (C), Diabetic Non-glucose Controlled group (DNC) and Diabetic glucose Controlled group (DC). RESULTS: Glucose control was achieved using Insulman Rapid (average daily glucose level <10 mmol/L). 18 Sprague-Dawley rats underwent a dorsal skin wound incision and 10 days later were killed. Fresh and fixed wound tensile strength, hydroxyproline and transforming growth factor beta-1 levels were improved in the DC group when compared to the DNC group. The quantity of fibroblasts present was similar in each group. CONCLUSION: This study demonstrates the impact that diabetes has on acute wound healing and suggests that wound modulating agents must be tested in both the tightly glucose-controlled as well as the poorly glucose-controlled diabetic animal models prior to proceeding with translational clinical studies.en_GB
dc.language.isoengen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshBlood Glucose/*analysisen_GB
dc.subject.meshBody Fluids/chemistryen_GB
dc.subject.meshDiabetes Mellitus, Experimental/blood/*physiopathologyen_GB
dc.subject.meshFibroblasts/metabolismen_GB
dc.subject.meshHydroxyproline/analysisen_GB
dc.subject.meshImmunohistochemistryen_GB
dc.subject.meshMaleen_GB
dc.subject.meshModels, Animalen_GB
dc.subject.meshRatsen_GB
dc.subject.meshRats, Sprague-Dawleyen_GB
dc.subject.meshTensile Strengthen_GB
dc.subject.meshTransforming Growth Factor beta1/analysisen_GB
dc.subject.meshWound Healing/*physiologyen_GB
dc.titleTight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model.en_GB
dc.contributor.departmentDepartment of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital,, Dublin, Ireland. jbarryosullivan@mac.comen_GB
dc.identifier.journalIrish journal of medical scienceen_GB
dc.description.provinceLeinster-

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