Measurement of the unfolded protein response (UPR) in monocytes.

Hdl Handle:
http://hdl.handle.net/10147/207173
Title:
Measurement of the unfolded protein response (UPR) in monocytes.
Authors:
Carroll, Tomas P; Greene, Catherine M; McElvaney, Noel G
Affiliation:
Department of Medicine, Royal College of Surgeons in Ireland, Education and, Research Centre, Beaumont Hospital, Dublin, Ireland.
Citation:
Methods Enzymol. 2011;489:83-95.
Journal:
Methods in enzymology
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207173
DOI:
10.1016/B978-0-12-385116-1.00005-4
PubMed ID:
21266225
Abstract:
In mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.
Language:
eng
MeSH:
DNA-Binding Proteins/genetics; Endoplasmic Reticulum/*metabolism; Enzyme-Linked Immunosorbent Assay/methods; Humans; Interleukin-8/analysis; Monocytes/*metabolism; RNA, Messenger/metabolism; Transcription Factors/genetics; *Unfolded Protein Response
ISSN:
1557-7988 (Electronic); 0076-6879 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorCarroll, Tomas Pen_GB
dc.contributor.authorGreene, Catherine Men_GB
dc.contributor.authorMcElvaney, Noel Gen_GB
dc.date.accessioned2012-02-01T10:01:04Z-
dc.date.available2012-02-01T10:01:04Z-
dc.date.issued2012-02-01T10:01:04Z-
dc.identifier.citationMethods Enzymol. 2011;489:83-95.en_GB
dc.identifier.issn1557-7988 (Electronic)en_GB
dc.identifier.issn0076-6879 (Linking)en_GB
dc.identifier.pmid21266225en_GB
dc.identifier.doi10.1016/B978-0-12-385116-1.00005-4en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207173-
dc.description.abstractIn mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.en_GB
dc.language.isoengen_GB
dc.subject.meshDNA-Binding Proteins/geneticsen_GB
dc.subject.meshEndoplasmic Reticulum/*metabolismen_GB
dc.subject.meshEnzyme-Linked Immunosorbent Assay/methodsen_GB
dc.subject.meshHumansen_GB
dc.subject.meshInterleukin-8/analysisen_GB
dc.subject.meshMonocytes/*metabolismen_GB
dc.subject.meshRNA, Messenger/metabolismen_GB
dc.subject.meshTranscription Factors/geneticsen_GB
dc.subject.mesh*Unfolded Protein Responseen_GB
dc.titleMeasurement of the unfolded protein response (UPR) in monocytes.en_GB
dc.contributor.departmentDepartment of Medicine, Royal College of Surgeons in Ireland, Education and, Research Centre, Beaumont Hospital, Dublin, Ireland.en_GB
dc.identifier.journalMethods in enzymologyen_GB
dc.description.provinceLeinster-

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