Purinergic receptor antagonists inhibit odorant-mediated CREB phosphorylation in sustentacular cells of mouse olfactory epithelium.

Hdl Handle:
http://hdl.handle.net/10147/207003
Title:
Purinergic receptor antagonists inhibit odorant-mediated CREB phosphorylation in sustentacular cells of mouse olfactory epithelium.
Authors:
Dooley, Ruth; Mashukova, Anastasia; Toetter, Bastian; Hatt, Hanns; Neuhaus, Eva M
Affiliation:
Department of Molecular Medicine, Royal College of Surgeons in Ireland, Beaumont , Hospital, Dublin, Ireland.
Citation:
BMC Neurosci. 2011 Aug 22;12:86.
Journal:
BMC neuroscience
Issue Date:
1-Feb-2012
URI:
http://hdl.handle.net/10147/207003
DOI:
10.1186/1471-2202-12-86
PubMed ID:
21859486
Abstract:
BACKGROUND: Extracellular nucleotides have long been known to play neuromodulatory roles and to be involved in intercellular signalling. In the olfactory system, ATP is released by olfactory neurons, and exogenous ATP can evoke an increase in intracellular calcium concentration in sustentacular cells, the nonneuronal supporting cells of the olfactory epithelium. Here we investigate the hypothesis that olfactory neurons communicate with sustentacular cells via extracellular ATP and purinergic receptor activation. RESULTS: Here we show that exposure of mice to a mixture of odorants induced a significant increase in the levels of the transcription factor CREB phosphorylated at Ser-133 in the nuclei of both olfactory sensory neurons and sustentacular cells. This activation was dependent on adenylyl cyclase III-mediated olfactory signaling and on activation of P2Y purinergic receptors on sustentacular cells. Purinergic receptor antagonists inhibited odorant-dependent CREB phosphorylation specifically in the nuclei of the sustentacular cells. CONCLUSION: Our results point to a possible role for extracellular nucleotides in mediating intercellular communication between the neurons and sustentacular cells of the olfactory epithelium in response to odorant exposure. Maintenance of extracellular ionic gradients and metabolism of noxious chemicals by sustentacular cells may therefore be regulated in an odorant-dependent manner by olfactory sensory neurons.
Language:
eng
ISSN:
1471-2202 (Electronic); 1471-2202 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorDooley, Ruthen_GB
dc.contributor.authorMashukova, Anastasiaen_GB
dc.contributor.authorToetter, Bastianen_GB
dc.contributor.authorHatt, Hannsen_GB
dc.contributor.authorNeuhaus, Eva Men_GB
dc.date.accessioned2012-02-01T09:57:23Z-
dc.date.available2012-02-01T09:57:23Z-
dc.date.issued2012-02-01T09:57:23Z-
dc.identifier.citationBMC Neurosci. 2011 Aug 22;12:86.en_GB
dc.identifier.issn1471-2202 (Electronic)en_GB
dc.identifier.issn1471-2202 (Linking)en_GB
dc.identifier.pmid21859486en_GB
dc.identifier.doi10.1186/1471-2202-12-86en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207003-
dc.description.abstractBACKGROUND: Extracellular nucleotides have long been known to play neuromodulatory roles and to be involved in intercellular signalling. In the olfactory system, ATP is released by olfactory neurons, and exogenous ATP can evoke an increase in intracellular calcium concentration in sustentacular cells, the nonneuronal supporting cells of the olfactory epithelium. Here we investigate the hypothesis that olfactory neurons communicate with sustentacular cells via extracellular ATP and purinergic receptor activation. RESULTS: Here we show that exposure of mice to a mixture of odorants induced a significant increase in the levels of the transcription factor CREB phosphorylated at Ser-133 in the nuclei of both olfactory sensory neurons and sustentacular cells. This activation was dependent on adenylyl cyclase III-mediated olfactory signaling and on activation of P2Y purinergic receptors on sustentacular cells. Purinergic receptor antagonists inhibited odorant-dependent CREB phosphorylation specifically in the nuclei of the sustentacular cells. CONCLUSION: Our results point to a possible role for extracellular nucleotides in mediating intercellular communication between the neurons and sustentacular cells of the olfactory epithelium in response to odorant exposure. Maintenance of extracellular ionic gradients and metabolism of noxious chemicals by sustentacular cells may therefore be regulated in an odorant-dependent manner by olfactory sensory neurons.en_GB
dc.language.isoengen_GB
dc.titlePurinergic receptor antagonists inhibit odorant-mediated CREB phosphorylation in sustentacular cells of mouse olfactory epithelium.en_GB
dc.contributor.departmentDepartment of Molecular Medicine, Royal College of Surgeons in Ireland, Beaumont , Hospital, Dublin, Ireland.en_GB
dc.identifier.journalBMC neuroscienceen_GB
dc.description.provinceLeinster-

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