The use of Listeria monocytogenes as a DNA delivery vector for cancer gene therapy.

Hdl Handle:
http://hdl.handle.net/10147/206382
Title:
The use of Listeria monocytogenes as a DNA delivery vector for cancer gene therapy.
Authors:
Tangney, Mark; van Pijkeren, Jan Peter; Gahan, Cormac G M
Affiliation:
Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr., Laboratory, University College Cork, Cork, Ireland.
Citation:
Bioeng Bugs. 2010 Jul-Aug;1(4):284-7. Epub 2010 Mar 7.
Journal:
Bioengineered bugs
Issue Date:
31-Jan-2012
URI:
http://hdl.handle.net/10147/206382
DOI:
10.4161/bbug.1.4.11725
PubMed ID:
21327062
Abstract:
Listeria monocytogenes is an intracellular pathogen that lyses the phagosomal vacuole of infected cells, proliferates in the host cell cytoplasm and can actively enter adjacent cells. The pathogen is therefore well suited to exploitation as a vector for the delivery of DNA to target cells as the lifecycle favors cellular targeting with vector amplification and the potential for cell-to-cell spread. We have recently demonstrated DNA transfer by L. monocytogenes in growing tumors in murine models. Our approach exploited an ampicillin sensitive stain of L. monocytogenes which can be lysed through systemic administration of ampicillin to facilitate release of plasmid DNA for expression by infected mammalian cells. Here, we discuss the implications of this technology and the potential for future improvements of the system.
Language:
eng
MeSH:
Animals; Cell Line, Tumor; Gene Therapy/*methods; Genetic Vectors/*genetics; Humans; Listeria monocytogenes/*genetics; Mice; Mice, Nude; Mice, Transgenic; Neoplasms/*therapy; Xenograft Model Antitumor Assays
ISSN:
1949-1026 (Electronic); 1949-1018 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorTangney, Marken_GB
dc.contributor.authorvan Pijkeren, Jan Peteren_GB
dc.contributor.authorGahan, Cormac G Men_GB
dc.date.accessioned2012-01-31T16:39:32Z-
dc.date.available2012-01-31T16:39:32Z-
dc.date.issued2012-01-31T16:39:32Z-
dc.identifier.citationBioeng Bugs. 2010 Jul-Aug;1(4):284-7. Epub 2010 Mar 7.en_GB
dc.identifier.issn1949-1026 (Electronic)en_GB
dc.identifier.issn1949-1018 (Linking)en_GB
dc.identifier.pmid21327062en_GB
dc.identifier.doi10.4161/bbug.1.4.11725en_GB
dc.identifier.urihttp://hdl.handle.net/10147/206382-
dc.description.abstractListeria monocytogenes is an intracellular pathogen that lyses the phagosomal vacuole of infected cells, proliferates in the host cell cytoplasm and can actively enter adjacent cells. The pathogen is therefore well suited to exploitation as a vector for the delivery of DNA to target cells as the lifecycle favors cellular targeting with vector amplification and the potential for cell-to-cell spread. We have recently demonstrated DNA transfer by L. monocytogenes in growing tumors in murine models. Our approach exploited an ampicillin sensitive stain of L. monocytogenes which can be lysed through systemic administration of ampicillin to facilitate release of plasmid DNA for expression by infected mammalian cells. Here, we discuss the implications of this technology and the potential for future improvements of the system.en_GB
dc.language.isoengen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshCell Line, Tumoren_GB
dc.subject.meshGene Therapy/*methodsen_GB
dc.subject.meshGenetic Vectors/*geneticsen_GB
dc.subject.meshHumansen_GB
dc.subject.meshListeria monocytogenes/*geneticsen_GB
dc.subject.meshMiceen_GB
dc.subject.meshMice, Nudeen_GB
dc.subject.meshMice, Transgenicen_GB
dc.subject.meshNeoplasms/*therapyen_GB
dc.subject.meshXenograft Model Antitumor Assaysen_GB
dc.titleThe use of Listeria monocytogenes as a DNA delivery vector for cancer gene therapy.en_GB
dc.contributor.departmentCork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr., Laboratory, University College Cork, Cork, Ireland.en_GB
dc.identifier.journalBioengineered bugsen_GB
dc.description.provinceMunster-

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