Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes.

Hdl Handle:
http://hdl.handle.net/10147/206298
Title:
Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes.
Authors:
Davis, Niall F; Mooney, Rory; Piterina, Anna V; Callanan, Anthony; McGuire, Barry B; Flood, Hugh D; McGloughlin, Tim M
Affiliation:
Department of Urology, Mid-Western Regional Hospital, Limerick, Ireland., nialldavis2001@yahoo.com
Citation:
Urology. 2011 Oct;78(4):954-60.
Journal:
Urology
Issue Date:
31-Jan-2012
URI:
http://hdl.handle.net/10147/206298
DOI:
10.1016/j.urology.2011.06.036
PubMed ID:
21982016
Abstract:
OBJECTIVE: To design and construct a urinary bladder bioreactor for urologic tissue-engineering purposes and to compare the viability and proliferative activity of cell-seeded extracellular matrix scaffolds cultured in the bioreactor with conventional static growth conditions. MATERIALS AND METHODS: A urinary bladder bioreactor was designed and constructed to replicate physiologic bladder dynamics. The bioreactor mimicked the filling pressures of the human bladder by way of a cyclical low-delivery pressure regulator. In addition, cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds in the bioreactor and in static growth conditions for 5 consecutive days. The attachment, viability, and proliferative potential were assessed and compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4',6-diamidino-2-phenylindole staining. RESULTS: No significant difference in cell viability was identified between both experimental groups after 3 days of culture (P = .06). By day 4, the number of viable UCs was significantly greater in the bioreactor compared with the number cultured under static conditions (P = .009). A significant difference in UC viability was also present after 5 days of culture between the bioreactor and static group (P = .006). Viability/cytotoxicity assays performed on day 5 also confirmed the viability of UCs in both experimental groups. CONCLUSION: Significantly greater UC growth occurred on the extracellular matrix scaffolds cultured in the bioreactor compared with conventional static laboratory conditions after 3 days of culture. Our initial bioreactor prototype might be helpful for permitting additional advances in urinary bladder bioreactor technology.
Language:
eng
MeSH:
Animals; *Bioreactors; Cell Membrane/metabolism; Cell Proliferation; Cell Survival; Coculture Techniques; Esterases/metabolism; Extracellular Matrix/metabolism; Humans; Microscopy, Electron, Scanning; Swine; Time Factors; Tissue Engineering/*methods; *Tissue Scaffolds; Urinary Bladder/*surgery; Urologic Surgical Procedures/*methods; Urothelium/cytology
ISSN:
1527-9995 (Electronic); 0090-4295 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorDavis, Niall Fen_GB
dc.contributor.authorMooney, Roryen_GB
dc.contributor.authorPiterina, Anna Ven_GB
dc.contributor.authorCallanan, Anthonyen_GB
dc.contributor.authorMcGuire, Barry Ben_GB
dc.contributor.authorFlood, Hugh Den_GB
dc.contributor.authorMcGloughlin, Tim Men_GB
dc.date.accessioned2012-01-31T16:46:17Z-
dc.date.available2012-01-31T16:46:17Z-
dc.date.issued2012-01-31T16:46:17Z-
dc.identifier.citationUrology. 2011 Oct;78(4):954-60.en_GB
dc.identifier.issn1527-9995 (Electronic)en_GB
dc.identifier.issn0090-4295 (Linking)en_GB
dc.identifier.pmid21982016en_GB
dc.identifier.doi10.1016/j.urology.2011.06.036en_GB
dc.identifier.urihttp://hdl.handle.net/10147/206298-
dc.description.abstractOBJECTIVE: To design and construct a urinary bladder bioreactor for urologic tissue-engineering purposes and to compare the viability and proliferative activity of cell-seeded extracellular matrix scaffolds cultured in the bioreactor with conventional static growth conditions. MATERIALS AND METHODS: A urinary bladder bioreactor was designed and constructed to replicate physiologic bladder dynamics. The bioreactor mimicked the filling pressures of the human bladder by way of a cyclical low-delivery pressure regulator. In addition, cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds in the bioreactor and in static growth conditions for 5 consecutive days. The attachment, viability, and proliferative potential were assessed and compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4',6-diamidino-2-phenylindole staining. RESULTS: No significant difference in cell viability was identified between both experimental groups after 3 days of culture (P = .06). By day 4, the number of viable UCs was significantly greater in the bioreactor compared with the number cultured under static conditions (P = .009). A significant difference in UC viability was also present after 5 days of culture between the bioreactor and static group (P = .006). Viability/cytotoxicity assays performed on day 5 also confirmed the viability of UCs in both experimental groups. CONCLUSION: Significantly greater UC growth occurred on the extracellular matrix scaffolds cultured in the bioreactor compared with conventional static laboratory conditions after 3 days of culture. Our initial bioreactor prototype might be helpful for permitting additional advances in urinary bladder bioreactor technology.en_GB
dc.language.isoengen_GB
dc.subject.meshAnimalsen_GB
dc.subject.mesh*Bioreactorsen_GB
dc.subject.meshCell Membrane/metabolismen_GB
dc.subject.meshCell Proliferationen_GB
dc.subject.meshCell Survivalen_GB
dc.subject.meshCoculture Techniquesen_GB
dc.subject.meshEsterases/metabolismen_GB
dc.subject.meshExtracellular Matrix/metabolismen_GB
dc.subject.meshHumansen_GB
dc.subject.meshMicroscopy, Electron, Scanningen_GB
dc.subject.meshSwineen_GB
dc.subject.meshTime Factorsen_GB
dc.subject.meshTissue Engineering/*methodsen_GB
dc.subject.mesh*Tissue Scaffoldsen_GB
dc.subject.meshUrinary Bladder/*surgeryen_GB
dc.subject.meshUrologic Surgical Procedures/*methodsen_GB
dc.subject.meshUrothelium/cytologyen_GB
dc.titleConstruction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes.en_GB
dc.contributor.departmentDepartment of Urology, Mid-Western Regional Hospital, Limerick, Ireland., nialldavis2001@yahoo.comen_GB
dc.identifier.journalUrologyen_GB
dc.description.provinceMunster-
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