Ex vivo culture of patient tissue & examination of gene delivery.

Hdl Handle:
http://hdl.handle.net/10147/206263
Title:
Ex vivo culture of patient tissue & examination of gene delivery.
Authors:
Rajendran, Simon; Salwa, Slawomir; Gao, Xuefeng; Tabirca, Sabin; O'Hanlon, Deirdre; O'Sullivan, Gerald C; Tangney, Mark
Affiliation:
Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr., Laboratory, University College Cork.
Citation:
J Vis Exp. 2010 Dec 20;(46). pii: 2378. doi: 10.3791/2378.
Journal:
Journal of visualized experiments : JoVE
Issue Date:
31-Jan-2012
URI:
http://hdl.handle.net/10147/206263
DOI:
10.3791/2378
PubMed ID:
21326169
Abstract:
This video describes the use of patient tissue as an ex vivo model for the study of gene delivery. Fresh patient tissue obtained at the time of surgery is sliced and maintained in culture. The ex vivo model system allows for the physical delivery of genes into intact patient tissue and gene expression is analysed by bioluminescence imaging using the IVIS detection system. The bioluminescent detection system demonstrates rapid and accurate quantification of gene expression within individual slices without the need for tissue sacrifice. This slice tissue culture system may be used in a variety of tissue types including normal and malignant tissue and allows us to study the effects of the heterogeneous nature of intact tissue and the high degree of variability between individual patients. This model system could be used in certain situations as an alternative to animal models and as a complementary preclinical mode prior to entering clinical trial.
Language:
eng
MeSH:
*Gene Transfer Techniques; Humans; Luminescent Measurements/*methods; Tissue Culture Techniques/*methods
ISSN:
1940-087X (Electronic); 1940-087X (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorRajendran, Simonen_GB
dc.contributor.authorSalwa, Slawomiren_GB
dc.contributor.authorGao, Xuefengen_GB
dc.contributor.authorTabirca, Sabinen_GB
dc.contributor.authorO'Hanlon, Deirdreen_GB
dc.contributor.authorO'Sullivan, Gerald Cen_GB
dc.contributor.authorTangney, Marken_GB
dc.date.accessioned2012-01-31T16:39:35Z-
dc.date.available2012-01-31T16:39:35Z-
dc.date.issued2012-01-31T16:39:35Z-
dc.identifier.citationJ Vis Exp. 2010 Dec 20;(46). pii: 2378. doi: 10.3791/2378.en_GB
dc.identifier.issn1940-087X (Electronic)en_GB
dc.identifier.issn1940-087X (Linking)en_GB
dc.identifier.pmid21326169en_GB
dc.identifier.doi10.3791/2378en_GB
dc.identifier.urihttp://hdl.handle.net/10147/206263-
dc.description.abstractThis video describes the use of patient tissue as an ex vivo model for the study of gene delivery. Fresh patient tissue obtained at the time of surgery is sliced and maintained in culture. The ex vivo model system allows for the physical delivery of genes into intact patient tissue and gene expression is analysed by bioluminescence imaging using the IVIS detection system. The bioluminescent detection system demonstrates rapid and accurate quantification of gene expression within individual slices without the need for tissue sacrifice. This slice tissue culture system may be used in a variety of tissue types including normal and malignant tissue and allows us to study the effects of the heterogeneous nature of intact tissue and the high degree of variability between individual patients. This model system could be used in certain situations as an alternative to animal models and as a complementary preclinical mode prior to entering clinical trial.en_GB
dc.language.isoengen_GB
dc.subject.mesh*Gene Transfer Techniquesen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLuminescent Measurements/*methodsen_GB
dc.subject.meshTissue Culture Techniques/*methodsen_GB
dc.titleEx vivo culture of patient tissue & examination of gene delivery.en_GB
dc.contributor.departmentCork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr., Laboratory, University College Cork.en_GB
dc.identifier.journalJournal of visualized experiments : JoVEen_GB
dc.description.provinceMunster-

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