Plasma-mediated vascular dysfunction in the reduced uterine perfusion pressure model of preeclampsia: a microvascular characterization.

Hdl Handle:
http://hdl.handle.net/10147/206209
Title:
Plasma-mediated vascular dysfunction in the reduced uterine perfusion pressure model of preeclampsia: a microvascular characterization.
Authors:
Walsh, Sarah K; English, Fred A; Johns, Edward J; Kenny, Louise C
Affiliation:
Anu Research Centre, Department of Obstetrics and Gynaecology, University College, Cork, Cork University Maternity Hospital, Wilton, Cork, Ireland. S.Walsh@ucc.ie
Citation:
Hypertension. 2009 Aug;54(2):345-51. Epub 2009 Jun 29.
Journal:
Hypertension
Issue Date:
31-Jan-2012
URI:
http://hdl.handle.net/10147/206209
DOI:
10.1161/HYPERTENSIONAHA.109.132191
PubMed ID:
19564546
Abstract:
Preeclampsia is associated with widespread maternal vascular dysfunction, which is thought to be mediated by circulating factor(s). The aim of the study was to characterize vascular function in the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia and to investigate the role of plasma factors in mediating any observed changes in vascular reactivity. Mean arterial blood pressure and vascular function were measured in RUPP and control rats. Mesenteric vessels from both virgin and pregnant rats were exposed for 1 hour or overnight to plasma from both RUPP and control rats and their vascular function assessed. RUPP rats were characterized by severe hypertension, restricted fetal growth, and reduced placental weight (P<0.001). Vasorelaxation was impaired in resistance vessels from RUPP compared with control rats (acetylcholine: R(max) 70+/-3 versus 92+/-1 [NP] and 93+/-3% [sham], P<0.01; bradykinin: 40+/-2 versus 62+/-2 [NP] and 59+/-4% [sham], P<0.001). Incubation of vessels from pregnant (but not virgin) animals with RUPP plasma overnight resulted in an attenuation of vasorelaxant responses (acetylcholine: 63+/-7 versus 86+/-2%, P<0.05; bradykinin: 35+/-5 versus 55+/-6%, P<0.001). The residual relaxant response in RUPP plasma-treated vessels was not further attenuated after treatment with N(omega)-nitro-l-arginine methyl ester (acetylcholine: 57+/-7 versus 63+/-7%, ns; bradykinin: 37+/-5 versus 35+/-5%, ns). The RUPP rat model is characterized by an impaired response to vasodilators which may be attributable to one or more circulating factors. This plasma-mediated endothelial dysfunction appears to be a pregnancy-dependent effect. Furthermore, nitric oxide-mediated vasorelaxation appears to be absent in RUPP plasma-treated vessels.
Language:
eng
MeSH:
Analysis of Variance; Animals; Blood Pressure Determination; Disease Models, Animal; Endothelium, Vascular/physiology; Female; Microcirculation/drug effects/*physiology; NG-Nitroarginine Methyl Ester/*pharmacology; Nitric Oxide/*metabolism; Perfusion/methods; Placenta/blood supply; Pre-Eclampsia/*physiopathology; Pregnancy; Pressure; Probability; Random Allocation; Rats; Rats, Sprague-Dawley; Risk Factors; Uterus/*blood supply; Vasoconstriction/drug effects/physiology; Vasodilation/drug effects/physiology
ISSN:
1524-4563 (Electronic); 0194-911X (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorWalsh, Sarah Ken_GB
dc.contributor.authorEnglish, Fred Aen_GB
dc.contributor.authorJohns, Edward Jen_GB
dc.contributor.authorKenny, Louise Cen_GB
dc.date.accessioned2012-01-31T16:42:27Z-
dc.date.available2012-01-31T16:42:27Z-
dc.date.issued2012-01-31T16:42:27Z-
dc.identifier.citationHypertension. 2009 Aug;54(2):345-51. Epub 2009 Jun 29.en_GB
dc.identifier.issn1524-4563 (Electronic)en_GB
dc.identifier.issn0194-911X (Linking)en_GB
dc.identifier.pmid19564546en_GB
dc.identifier.doi10.1161/HYPERTENSIONAHA.109.132191en_GB
dc.identifier.urihttp://hdl.handle.net/10147/206209-
dc.description.abstractPreeclampsia is associated with widespread maternal vascular dysfunction, which is thought to be mediated by circulating factor(s). The aim of the study was to characterize vascular function in the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia and to investigate the role of plasma factors in mediating any observed changes in vascular reactivity. Mean arterial blood pressure and vascular function were measured in RUPP and control rats. Mesenteric vessels from both virgin and pregnant rats were exposed for 1 hour or overnight to plasma from both RUPP and control rats and their vascular function assessed. RUPP rats were characterized by severe hypertension, restricted fetal growth, and reduced placental weight (P<0.001). Vasorelaxation was impaired in resistance vessels from RUPP compared with control rats (acetylcholine: R(max) 70+/-3 versus 92+/-1 [NP] and 93+/-3% [sham], P<0.01; bradykinin: 40+/-2 versus 62+/-2 [NP] and 59+/-4% [sham], P<0.001). Incubation of vessels from pregnant (but not virgin) animals with RUPP plasma overnight resulted in an attenuation of vasorelaxant responses (acetylcholine: 63+/-7 versus 86+/-2%, P<0.05; bradykinin: 35+/-5 versus 55+/-6%, P<0.001). The residual relaxant response in RUPP plasma-treated vessels was not further attenuated after treatment with N(omega)-nitro-l-arginine methyl ester (acetylcholine: 57+/-7 versus 63+/-7%, ns; bradykinin: 37+/-5 versus 35+/-5%, ns). The RUPP rat model is characterized by an impaired response to vasodilators which may be attributable to one or more circulating factors. This plasma-mediated endothelial dysfunction appears to be a pregnancy-dependent effect. Furthermore, nitric oxide-mediated vasorelaxation appears to be absent in RUPP plasma-treated vessels.en_GB
dc.language.isoengen_GB
dc.subject.meshAnalysis of Varianceen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshBlood Pressure Determinationen_GB
dc.subject.meshDisease Models, Animalen_GB
dc.subject.meshEndothelium, Vascular/physiologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshMicrocirculation/drug effects/*physiologyen_GB
dc.subject.meshNG-Nitroarginine Methyl Ester/*pharmacologyen_GB
dc.subject.meshNitric Oxide/*metabolismen_GB
dc.subject.meshPerfusion/methodsen_GB
dc.subject.meshPlacenta/blood supplyen_GB
dc.subject.meshPre-Eclampsia/*physiopathologyen_GB
dc.subject.meshPregnancyen_GB
dc.subject.meshPressureen_GB
dc.subject.meshProbabilityen_GB
dc.subject.meshRandom Allocationen_GB
dc.subject.meshRatsen_GB
dc.subject.meshRats, Sprague-Dawleyen_GB
dc.subject.meshRisk Factorsen_GB
dc.subject.meshUterus/*blood supplyen_GB
dc.subject.meshVasoconstriction/drug effects/physiologyen_GB
dc.subject.meshVasodilation/drug effects/physiologyen_GB
dc.titlePlasma-mediated vascular dysfunction in the reduced uterine perfusion pressure model of preeclampsia: a microvascular characterization.en_GB
dc.contributor.departmentAnu Research Centre, Department of Obstetrics and Gynaecology, University College, Cork, Cork University Maternity Hospital, Wilton, Cork, Ireland. S.Walsh@ucc.ieen_GB
dc.identifier.journalHypertensionen_GB
dc.description.provinceMunster-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.