Soluble Flt-1 and PlGF: new markers of early pregnancy loss?

Hdl Handle:
http://hdl.handle.net/10147/205805
Title:
Soluble Flt-1 and PlGF: new markers of early pregnancy loss?
Authors:
Muttukrishna, Shanthi; Swer, Michelle; Suri, Sangeeta; Jamil, Amna; Calleja-Agius, Jean; Gangooly, Subrata; Ludlow, Helen; Jurkovic, Davor; Jauniaux, Eric
Affiliation:
Department of Obstetrics and Gynaecology, Anu Research Centre, University College, Cork, Cork University Maternity Hospital, Wilton, Cork, Republic of Ireland., S.Muttukrishna@ucc.ie
Citation:
PLoS One. 2011 Mar 23;6(3):e18041.
Journal:
PloS one
Issue Date:
31-Jan-2012
URI:
http://hdl.handle.net/10147/205805
DOI:
10.1371/journal.pone.0018041
PubMed ID:
21448460
Abstract:
Recent data have indicated a relationship between placental oxygen and angiogenic protein levels in the first trimester of normal pregnancies. Our objective was to investigate if maternal serum levels of angiogenic factors Soluble vascular endothelial growth factor (VEGF) receptor 1 (sFlt-1), soluble Endoglin and placental growth factor (PlGF) are altered in women with symptoms of threatened miscarriage (TM) and if they are predictive of a subsequent miscarriage. Blood samples were collected at 6-10 weeks from women presenting with TM (n = 40), from asymptomatic controls (n = 32) and from non- pregnant women in their luteal phase (n = 14). All samples were assayed for serum level of sFLT-1, PlGF, sEndoglin and HSP70 using commercial ELISAs. Samples were analysed retrospectively on the basis of pregnancy outcome. TM group included 21 women with a normal pregnancy outcome and 19 with subsequent complete miscarriage. The latter subgroup had significantly lower mean maternal serum (MS) sFlt-1 (83%, P<0.001) and PlGF (44%, P<0.001) compared to those with a normal pregnancy outcome. Asymptomatic control pregnant women had similar MS levels of sFlt-1 and PlGF compared to the TM patients with a normal outcome. The mean MS sFlt-1 (>10 fold) and MS PlGF ( approximately 2 fold) levels were significantly (P<0.001) higher in control pregnant women compared to the non-pregnant group in the luteal phase of the menstrual cycle. Soluble Endoglin was not altered in the normal pregnant women compared to non pregnant women, although lower in the TM subgroup with a subsequent miscarriage ( approximately 25%, P<0.001) compared to TM with a live birth. There was no significant difference in the mean MS HSP 70 levels between the different groups. This study shows that sFlt1 and PlGF MS levels are increased by several folds in early pregnancy and that MS sFlt-1 and MS PlGF are markedly decreased in threatened miscarriage patients who subsequently have a miscarriage suggesting these proteins are sensitive predictive markers of subsequent pregnancy loss.
Language:
eng
MeSH:
Adult; Antigens, CD/blood; Biological Markers/blood; Demography; Embryo Loss/*blood; Female; HSP70 Heat-Shock Proteins/blood; Humans; Membrane Proteins/*blood; Pregnancy; Receptors, Cell Surface/blood; Solubility; Vascular Endothelial Growth Factor Receptor-1/*blood
ISSN:
1932-6203 (Electronic); 1932-6203 (Linking)

Full metadata record

DC FieldValue Language
dc.contributor.authorMuttukrishna, Shanthien_GB
dc.contributor.authorSwer, Michelleen_GB
dc.contributor.authorSuri, Sangeetaen_GB
dc.contributor.authorJamil, Amnaen_GB
dc.contributor.authorCalleja-Agius, Jeanen_GB
dc.contributor.authorGangooly, Subrataen_GB
dc.contributor.authorLudlow, Helenen_GB
dc.contributor.authorJurkovic, Davoren_GB
dc.contributor.authorJauniaux, Ericen_GB
dc.date.accessioned2012-01-31T16:42:38Z-
dc.date.available2012-01-31T16:42:38Z-
dc.date.issued2012-01-31T16:42:38Z-
dc.identifier.citationPLoS One. 2011 Mar 23;6(3):e18041.en_GB
dc.identifier.issn1932-6203 (Electronic)en_GB
dc.identifier.issn1932-6203 (Linking)en_GB
dc.identifier.pmid21448460en_GB
dc.identifier.doi10.1371/journal.pone.0018041en_GB
dc.identifier.urihttp://hdl.handle.net/10147/205805-
dc.description.abstractRecent data have indicated a relationship between placental oxygen and angiogenic protein levels in the first trimester of normal pregnancies. Our objective was to investigate if maternal serum levels of angiogenic factors Soluble vascular endothelial growth factor (VEGF) receptor 1 (sFlt-1), soluble Endoglin and placental growth factor (PlGF) are altered in women with symptoms of threatened miscarriage (TM) and if they are predictive of a subsequent miscarriage. Blood samples were collected at 6-10 weeks from women presenting with TM (n = 40), from asymptomatic controls (n = 32) and from non- pregnant women in their luteal phase (n = 14). All samples were assayed for serum level of sFLT-1, PlGF, sEndoglin and HSP70 using commercial ELISAs. Samples were analysed retrospectively on the basis of pregnancy outcome. TM group included 21 women with a normal pregnancy outcome and 19 with subsequent complete miscarriage. The latter subgroup had significantly lower mean maternal serum (MS) sFlt-1 (83%, P<0.001) and PlGF (44%, P<0.001) compared to those with a normal pregnancy outcome. Asymptomatic control pregnant women had similar MS levels of sFlt-1 and PlGF compared to the TM patients with a normal outcome. The mean MS sFlt-1 (>10 fold) and MS PlGF ( approximately 2 fold) levels were significantly (P<0.001) higher in control pregnant women compared to the non-pregnant group in the luteal phase of the menstrual cycle. Soluble Endoglin was not altered in the normal pregnant women compared to non pregnant women, although lower in the TM subgroup with a subsequent miscarriage ( approximately 25%, P<0.001) compared to TM with a live birth. There was no significant difference in the mean MS HSP 70 levels between the different groups. This study shows that sFlt1 and PlGF MS levels are increased by several folds in early pregnancy and that MS sFlt-1 and MS PlGF are markedly decreased in threatened miscarriage patients who subsequently have a miscarriage suggesting these proteins are sensitive predictive markers of subsequent pregnancy loss.en_GB
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAntigens, CD/blooden_GB
dc.subject.meshBiological Markers/blooden_GB
dc.subject.meshDemographyen_GB
dc.subject.meshEmbryo Loss/*blooden_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHSP70 Heat-Shock Proteins/blooden_GB
dc.subject.meshHumansen_GB
dc.subject.meshMembrane Proteins/*blooden_GB
dc.subject.meshPregnancyen_GB
dc.subject.meshReceptors, Cell Surface/blooden_GB
dc.subject.meshSolubilityen_GB
dc.subject.meshVascular Endothelial Growth Factor Receptor-1/*blooden_GB
dc.titleSoluble Flt-1 and PlGF: new markers of early pregnancy loss?en_GB
dc.contributor.departmentDepartment of Obstetrics and Gynaecology, Anu Research Centre, University College, Cork, Cork University Maternity Hospital, Wilton, Cork, Republic of Ireland., S.Muttukrishna@ucc.ieen_GB
dc.identifier.journalPloS oneen_GB
dc.description.provinceMunster-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.