Differential expression of toll-like receptors in patients with irritable bowel syndrome.

Hdl Handle:
http://hdl.handle.net/10147/201255
Title:
Differential expression of toll-like receptors in patients with irritable bowel syndrome.
Authors:
Brint, Elizabeth K; MacSharry, John; Fanning, Aine; Shanahan, Fergus; Quigley, Eamonn M M
Affiliation:
Department of Pathology, University College Cork, National University of Ireland, and Alimentary Pharmabiotic Centre, Cork University Hospital, Cork, Ireland. e.brint@ucc.ie
Citation:
Differential expression of toll-like receptors in patients with irritable bowel syndrome. 2011, 106 (2):329-36 Am. J. Gastroenterol.
Journal:
The American journal of gastroenterology
Issue Date:
Feb-2011
URI:
http://hdl.handle.net/10147/201255
DOI:
10.1038/ajg.2010.438
PubMed ID:
21102570
Abstract:
The pathogenesis of irritable bowel syndrome (IBS) is poorly understood. One contributory factor may be low-grade mucosal inflammation, perhaps initiated by the microbiota. Toll-like receptors (TLRs) are a family of pathogen-recognition receptors of the innate immune system. The aim of this study was to evaluate the potential involvement of TLRs in IBS to further understand the involvement of the innate immune system in this complex disorder.; The expression of TLRs was investigated in colonic biopsy samples obtained from 26 IBS patients and compared with 19 healthy controls. Protein expression of TLR4, TLR7, and TLR8 was confirmed by immunofluorescence and alterations in the TLR4 protein were confirmed by western blot.; Quantitative reverse transcriptase-PCR showed increased levels of TLR4 (P≤0.001) and TLR5 (P=0.0013) and decreased levels of TLR7 (P≤0.001) and TLR8 (P=0.0019) in IBS patients.; Our results support the presence of an immune engagement between the microbiota and the host in IBS; an interaction that involves innate immunity and could generate a low-grade inflammatory response. These findings could also offer an additional biomarker of the disease or a disease subset.
Item Type:
Article
Language:
en
Description:
OBJECTIVES: The pathogenesis of irritable bowel syndrome (IBS) is poorly understood. One contributory factor may be low-grade mucosal inflammation, perhaps initiated by the microbiota. Toll-like receptors (TLRs) are a family of pathogen-recognition receptors of the innate immune system. The aim of this study was to evaluate the potential involvement of TLRs in IBS to further understand the involvement of the innate immune system in this complex disorder. METHODS: The expression of TLRs was investigated in colonic biopsy samples obtained from 26 IBS patients and compared with 19 healthy controls. Protein expression of TLR4, TLR7, and TLR8 was confirmed by immunofluorescence and alterations in the TLR4 protein were confirmed by western blot. RESULTS: Quantitative reverse transcriptase-PCR showed increased levels of TLR4 (P≤0.001) and TLR5 (P=0.0013) and decreased levels of TLR7 (P≤0.001) and TLR8 (P=0.0019) in IBS patients. CONCLUSIONS: Our results support the presence of an immune engagement between the microbiota and the host in IBS; an interaction that involves innate immunity and could generate a low-grade inflammatory response. These findings could also offer an additional biomarker of the disease or a disease subset.
MeSH:
Adult; Analysis of Variance; Biopsy; Blotting, Western; Case-Control Studies; Female; Fluorescent Antibody Technique; Humans; Immunity, Innate; Irritable Bowel Syndrome; Microarray Analysis; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; Statistics, Nonparametric; Toll-Like Receptors
ISSN:
1572-0241

Full metadata record

DC FieldValue Language
dc.contributor.authorBrint, Elizabeth Ken
dc.contributor.authorMacSharry, Johnen
dc.contributor.authorFanning, Aineen
dc.contributor.authorShanahan, Fergusen
dc.contributor.authorQuigley, Eamonn M Men
dc.date.accessioned2012-01-10T14:05:06Z-
dc.date.available2012-01-10T14:05:06Z-
dc.date.issued2011-02-
dc.identifier.citationDifferential expression of toll-like receptors in patients with irritable bowel syndrome. 2011, 106 (2):329-36 Am. J. Gastroenterol.en
dc.identifier.issn1572-0241-
dc.identifier.pmid21102570-
dc.identifier.doi10.1038/ajg.2010.438-
dc.identifier.urihttp://hdl.handle.net/10147/201255-
dc.descriptionOBJECTIVES: The pathogenesis of irritable bowel syndrome (IBS) is poorly understood. One contributory factor may be low-grade mucosal inflammation, perhaps initiated by the microbiota. Toll-like receptors (TLRs) are a family of pathogen-recognition receptors of the innate immune system. The aim of this study was to evaluate the potential involvement of TLRs in IBS to further understand the involvement of the innate immune system in this complex disorder. METHODS: The expression of TLRs was investigated in colonic biopsy samples obtained from 26 IBS patients and compared with 19 healthy controls. Protein expression of TLR4, TLR7, and TLR8 was confirmed by immunofluorescence and alterations in the TLR4 protein were confirmed by western blot. RESULTS: Quantitative reverse transcriptase-PCR showed increased levels of TLR4 (P≤0.001) and TLR5 (P=0.0013) and decreased levels of TLR7 (P≤0.001) and TLR8 (P=0.0019) in IBS patients. CONCLUSIONS: Our results support the presence of an immune engagement between the microbiota and the host in IBS; an interaction that involves innate immunity and could generate a low-grade inflammatory response. These findings could also offer an additional biomarker of the disease or a disease subset.en
dc.description.abstractThe pathogenesis of irritable bowel syndrome (IBS) is poorly understood. One contributory factor may be low-grade mucosal inflammation, perhaps initiated by the microbiota. Toll-like receptors (TLRs) are a family of pathogen-recognition receptors of the innate immune system. The aim of this study was to evaluate the potential involvement of TLRs in IBS to further understand the involvement of the innate immune system in this complex disorder.-
dc.description.abstractThe expression of TLRs was investigated in colonic biopsy samples obtained from 26 IBS patients and compared with 19 healthy controls. Protein expression of TLR4, TLR7, and TLR8 was confirmed by immunofluorescence and alterations in the TLR4 protein were confirmed by western blot.-
dc.description.abstractQuantitative reverse transcriptase-PCR showed increased levels of TLR4 (P≤0.001) and TLR5 (P=0.0013) and decreased levels of TLR7 (P≤0.001) and TLR8 (P=0.0019) in IBS patients.-
dc.description.abstractOur results support the presence of an immune engagement between the microbiota and the host in IBS; an interaction that involves innate immunity and could generate a low-grade inflammatory response. These findings could also offer an additional biomarker of the disease or a disease subset.-
dc.language.isoenen
dc.subject.meshAdult-
dc.subject.meshAnalysis of Variance-
dc.subject.meshBiopsy-
dc.subject.meshBlotting, Western-
dc.subject.meshCase-Control Studies-
dc.subject.meshFemale-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshHumans-
dc.subject.meshImmunity, Innate-
dc.subject.meshIrritable Bowel Syndrome-
dc.subject.meshMicroarray Analysis-
dc.subject.meshMiddle Aged-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshStatistics, Nonparametric-
dc.subject.meshToll-Like Receptors-
dc.titleDifferential expression of toll-like receptors in patients with irritable bowel syndrome.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, University College Cork, National University of Ireland, and Alimentary Pharmabiotic Centre, Cork University Hospital, Cork, Ireland. e.brint@ucc.ieen
dc.identifier.journalThe American journal of gastroenterologyen
dc.description.provinceMunster-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.