Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.

Hdl Handle:
http://hdl.handle.net/10147/200337
Title:
Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.
Authors:
Kiely, R A; Lucey, B; Cotter, L
Affiliation:
Department of Medical Microbiology, Cork University Hospital, Cork, Ireland.
Citation:
Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland. 2010, 138 (2):286-91 Epidemiol. Infect.
Journal:
Epidemiology and infection
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10147/200337
DOI:
10.1017/S0950268809990392
PubMed ID:
19602300
Abstract:
The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.
Item Type:
Article
Language:
en
Description:
The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.
MeSH:
Adolescent; Adult; Anti-Bacterial Agents; Drug Resistance, Bacterial; Erythromycin; Female; Gene Expression Regulation, Bacterial; Genotype; Humans; Ireland; Microbial Sensitivity Tests; Middle Aged; Phenotype; Serotyping; Streptococcal Infections; Streptococcus agalactiae; Vagina; Vaginal Diseases; Young Adult
ISSN:
1469-4409

Full metadata record

DC FieldValue Language
dc.contributor.authorKiely, R Aen
dc.contributor.authorLucey, Ben
dc.contributor.authorCotter, Len
dc.date.accessioned2012-01-05T15:39:10Z-
dc.date.available2012-01-05T15:39:10Z-
dc.date.issued2010-02-
dc.identifier.citationAnalysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland. 2010, 138 (2):286-91 Epidemiol. Infect.en
dc.identifier.issn1469-4409-
dc.identifier.pmid19602300-
dc.identifier.doi10.1017/S0950268809990392-
dc.identifier.urihttp://hdl.handle.net/10147/200337-
dc.descriptionThe screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.en
dc.description.abstractThe screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.-
dc.language.isoenen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAnti-Bacterial Agents-
dc.subject.meshDrug Resistance, Bacterial-
dc.subject.meshErythromycin-
dc.subject.meshFemale-
dc.subject.meshGene Expression Regulation, Bacterial-
dc.subject.meshGenotype-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshMiddle Aged-
dc.subject.meshPhenotype-
dc.subject.meshSerotyping-
dc.subject.meshStreptococcal Infections-
dc.subject.meshStreptococcus agalactiae-
dc.subject.meshVagina-
dc.subject.meshVaginal Diseases-
dc.subject.meshYoung Adult-
dc.titleAnalysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, Cork University Hospital, Cork, Ireland.en
dc.identifier.journalEpidemiology and infectionen
dc.description.provinceMunster-

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