Mycobacterium avium subsp. Paratuberculosis (MAP) as a modifying factor in Crohn's disease.

Hdl Handle:
http://hdl.handle.net/10147/200281
Title:
Mycobacterium avium subsp. Paratuberculosis (MAP) as a modifying factor in Crohn's disease.
Authors:
Sibartie, Shomik; Scully, Paul; Keohane, John; O'Neill, Shaun; O'Mahony, Jim; O'Hanlon, Deirdre; Kirwan, William O; O'Mahony, Liam; Shanahan, Fergus
Affiliation:
Alimentary Pharmabiotic Centre, University College Cork, National University of Ireland, Cork, Ireland.
Citation:
Mycobacterium avium subsp. Paratuberculosis (MAP) as a modifying factor in Crohn's disease. 2010, 16 (2):296-304 Inflamm. Bowel Dis.
Journal:
Inflammatory bowel diseases
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10147/200281
DOI:
10.1002/ibd.21052
PubMed ID:
19824071
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1002/ibd.21052/pdf
Abstract:
Crohn's disease (CD) is a multifactorial syndrome with genetic and environmental contributions. Mycobacterium avium subspecies paratuberculosis (MAP) has been frequently isolated from mucosal tissues of patients with CD but the cellular immune response to this bacterium has been poorly described. Our aim was to examine the influence of MAP on T-cell proliferation and cytokine responses in patients with inflammatory bowel disease (IBD).; Peripheral blood mononuclear cells (PBMCs) and mesenteric lymph node cells (MLNCs) were obtained from IBD patients and non-IBD controls. PBMC T-cell proliferation in response to MAP was determined using CFSE labeling and flow cytometry. The specificity of cytokine responses to MAP was controlled by parallel exposure to Listeria monocytogenes (LM) or Salmonella typhimurium (ST).; Coincubation of PBMCs with MAP induced significantly more T-cell proliferation (P < 0.0001) in PBMCs isolated from CD patients compared to PBMCs obtained from ulcerative colitis (UC) patients or healthy volunteers. In addition, PBMCs from CD patients secreted significantly higher (P < 0.05) levels of tumor necrosis factor-alpha (TNF-alpha; 2302 +/- 230 pg/mL) and interleukin (IL)-10 (299 +/- 48 pg/mL) in response to MAP compared to UC patients (TNF-alpha: 1219 +/- 411 pg/mL; IL-10: 125 +/- 19 pg/mL) and controls (TNF-alpha: 1447 +/- 173 pg/mL; IL-10: 127 +/- 12 pg/mL). No difference in cytokine responses was observed in response to LM or ST. MLNCs from both CD and UC patients secreted significantly more TNF-alpha and IL-8 in response to MAP compared to MLNCs from non-IBD control patients.; Increased proliferation of T cells and an altered cytokine response suggest that prior exposure to MAP and engagement of the immune system is common in patients with CD. This does not imply causation but does support further examination of this bacterium as an environmental modifying factor.
Item Type:
Article
Language:
en
Description:
BACKGROUND: Crohn's disease (CD) is a multifactorial syndrome with genetic and environmental contributions. Mycobacterium avium subspecies paratuberculosis (MAP) has been frequently isolated from mucosal tissues of patients with CD but the cellular immune response to this bacterium has been poorly described. Our aim was to examine the influence of MAP on T-cell proliferation and cytokine responses in patients with inflammatory bowel disease (IBD). METHODS: Peripheral blood mononuclear cells (PBMCs) and mesenteric lymph node cells (MLNCs) were obtained from IBD patients and non-IBD controls. PBMC T-cell proliferation in response to MAP was determined using CFSE labeling and flow cytometry. The specificity of cytokine responses to MAP was controlled by parallel exposure to Listeria monocytogenes (LM) or Salmonella typhimurium (ST). RESULTS: Coincubation of PBMCs with MAP induced significantly more T-cell proliferation (P < 0.0001) in PBMCs isolated from CD patients compared to PBMCs obtained from ulcerative colitis (UC) patients or healthy volunteers. In addition, PBMCs from CD patients secreted significantly higher (P < 0.05) levels of tumor necrosis factor-alpha (TNF-alpha; 2302 +/- 230 pg/mL) and interleukin (IL)-10 (299 +/- 48 pg/mL) in response to MAP compared to UC patients (TNF-alpha: 1219 +/- 411 pg/mL; IL-10: 125 +/- 19 pg/mL) and controls (TNF-alpha: 1447 +/- 173 pg/mL; IL-10: 127 +/- 12 pg/mL). No difference in cytokine responses was observed in response to LM or ST. MLNCs from both CD and UC patients secreted significantly more TNF-alpha and IL-8 in response to MAP compared to MLNCs from non-IBD control patients. CONCLUSIONS: Increased proliferation of T cells and an altered cytokine response suggest that prior exposure to MAP and engagement of the immune system is common in patients with CD. This does not imply causation but does support further examination of this bacterium as an environmental modifying factor.
MeSH:
Adolescent; Adult; Aged; Aged, 80 and over; Crohn Disease; Cytokines; Female; Humans; Interleukin-10; Lymphocyte Activation; Male; Middle Aged; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis; T-Lymphocytes; Tumor Necrosis Factor-alpha; Young Adult
ISSN:
1536-4844

Full metadata record

DC FieldValue Language
dc.contributor.authorSibartie, Shomiken
dc.contributor.authorScully, Paulen
dc.contributor.authorKeohane, Johnen
dc.contributor.authorO'Neill, Shaunen
dc.contributor.authorO'Mahony, Jimen
dc.contributor.authorO'Hanlon, Deirdreen
dc.contributor.authorKirwan, William Oen
dc.contributor.authorO'Mahony, Liamen
dc.contributor.authorShanahan, Fergusen
dc.date.accessioned2012-01-05T15:42:52Z-
dc.date.available2012-01-05T15:42:52Z-
dc.date.issued2010-02-
dc.identifier.citationMycobacterium avium subsp. Paratuberculosis (MAP) as a modifying factor in Crohn's disease. 2010, 16 (2):296-304 Inflamm. Bowel Dis.en
dc.identifier.issn1536-4844-
dc.identifier.pmid19824071-
dc.identifier.doi10.1002/ibd.21052-
dc.identifier.urihttp://hdl.handle.net/10147/200281-
dc.descriptionBACKGROUND: Crohn's disease (CD) is a multifactorial syndrome with genetic and environmental contributions. Mycobacterium avium subspecies paratuberculosis (MAP) has been frequently isolated from mucosal tissues of patients with CD but the cellular immune response to this bacterium has been poorly described. Our aim was to examine the influence of MAP on T-cell proliferation and cytokine responses in patients with inflammatory bowel disease (IBD). METHODS: Peripheral blood mononuclear cells (PBMCs) and mesenteric lymph node cells (MLNCs) were obtained from IBD patients and non-IBD controls. PBMC T-cell proliferation in response to MAP was determined using CFSE labeling and flow cytometry. The specificity of cytokine responses to MAP was controlled by parallel exposure to Listeria monocytogenes (LM) or Salmonella typhimurium (ST). RESULTS: Coincubation of PBMCs with MAP induced significantly more T-cell proliferation (P < 0.0001) in PBMCs isolated from CD patients compared to PBMCs obtained from ulcerative colitis (UC) patients or healthy volunteers. In addition, PBMCs from CD patients secreted significantly higher (P < 0.05) levels of tumor necrosis factor-alpha (TNF-alpha; 2302 +/- 230 pg/mL) and interleukin (IL)-10 (299 +/- 48 pg/mL) in response to MAP compared to UC patients (TNF-alpha: 1219 +/- 411 pg/mL; IL-10: 125 +/- 19 pg/mL) and controls (TNF-alpha: 1447 +/- 173 pg/mL; IL-10: 127 +/- 12 pg/mL). No difference in cytokine responses was observed in response to LM or ST. MLNCs from both CD and UC patients secreted significantly more TNF-alpha and IL-8 in response to MAP compared to MLNCs from non-IBD control patients. CONCLUSIONS: Increased proliferation of T cells and an altered cytokine response suggest that prior exposure to MAP and engagement of the immune system is common in patients with CD. This does not imply causation but does support further examination of this bacterium as an environmental modifying factor.en
dc.description.abstractCrohn's disease (CD) is a multifactorial syndrome with genetic and environmental contributions. Mycobacterium avium subspecies paratuberculosis (MAP) has been frequently isolated from mucosal tissues of patients with CD but the cellular immune response to this bacterium has been poorly described. Our aim was to examine the influence of MAP on T-cell proliferation and cytokine responses in patients with inflammatory bowel disease (IBD).-
dc.description.abstractPeripheral blood mononuclear cells (PBMCs) and mesenteric lymph node cells (MLNCs) were obtained from IBD patients and non-IBD controls. PBMC T-cell proliferation in response to MAP was determined using CFSE labeling and flow cytometry. The specificity of cytokine responses to MAP was controlled by parallel exposure to Listeria monocytogenes (LM) or Salmonella typhimurium (ST).-
dc.description.abstractCoincubation of PBMCs with MAP induced significantly more T-cell proliferation (P < 0.0001) in PBMCs isolated from CD patients compared to PBMCs obtained from ulcerative colitis (UC) patients or healthy volunteers. In addition, PBMCs from CD patients secreted significantly higher (P < 0.05) levels of tumor necrosis factor-alpha (TNF-alpha; 2302 +/- 230 pg/mL) and interleukin (IL)-10 (299 +/- 48 pg/mL) in response to MAP compared to UC patients (TNF-alpha: 1219 +/- 411 pg/mL; IL-10: 125 +/- 19 pg/mL) and controls (TNF-alpha: 1447 +/- 173 pg/mL; IL-10: 127 +/- 12 pg/mL). No difference in cytokine responses was observed in response to LM or ST. MLNCs from both CD and UC patients secreted significantly more TNF-alpha and IL-8 in response to MAP compared to MLNCs from non-IBD control patients.-
dc.description.abstractIncreased proliferation of T cells and an altered cytokine response suggest that prior exposure to MAP and engagement of the immune system is common in patients with CD. This does not imply causation but does support further examination of this bacterium as an environmental modifying factor.-
dc.language.isoenen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/ibd.21052/pdfen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshCrohn Disease-
dc.subject.meshCytokines-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshInterleukin-10-
dc.subject.meshLymphocyte Activation-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMycobacterium avium subsp. paratuberculosis-
dc.subject.meshParatuberculosis-
dc.subject.meshT-Lymphocytes-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.subject.meshYoung Adult-
dc.titleMycobacterium avium subsp. Paratuberculosis (MAP) as a modifying factor in Crohn's disease.en
dc.typeArticleen
dc.contributor.departmentAlimentary Pharmabiotic Centre, University College Cork, National University of Ireland, Cork, Ireland.en
dc.identifier.journalInflammatory bowel diseasesen
dc.description.provinceMunster-

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