Food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish.

Hdl Handle:
http://hdl.handle.net/10147/200237
Title:
Food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish.
Authors:
Skrabáková, Zuzana; O'Halloran, John; van Pelt, Frank N A M; James, Kevin J
Affiliation:
PROTEOBIO (Mass Spectrometry Centre), Cork Institute of Technology, Bishopstown, Cork, Ireland.
Citation:
Food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish. 2010, 24 (20):2966-74 Rapid Commun. Mass Spectrom.
Publisher:
Wiley-Blackwell
Journal:
Rapid communications in mass spectrometry : RCM
Issue Date:
30-Oct-2010
URI:
http://hdl.handle.net/10147/200237
DOI:
10.1002/rcm.4724
PubMed ID:
20872629
Abstract:
The biotoxins, azaspiracids (AZAs), from marine phytoplankton accumulate in shellfish and affect human health by causing severe gastrointestinal disturbance, diarrhea, nausea and vomiting. Specific and sensitive methods have been developed and validated for the determination of the most commonly occurring azaspiracid analogs. An LTQ Orbitrap mass spectrometer is a hybrid instrument that combines linear ion trap (LIT) mass spectrometry (MS) with high-resolution Fourier transform (FT) MS and this was exploited to perform simultaneous ultra-high-resolution full-scan MS analysis and collision-induced dissociation (CID) tandem mass spectrometry (MS/MS). Using the highest mass resolution setting (100,000 FWHM) in full-scan mode, the methodology was validated for the determination of six AZAs in mussel (Mytilus galloprovincialis) tissue extracts. Ultra-high mass resolution, together with a narrow mass tolerance window of ±2 mDa, dramatically improved detection sensitivity. In addition to employing chromatographic resolution to distinguish between the isomeric azaspiracid analogs, AZA1/AZA6 and AZA4/AZA5, higher energy collisionally induced dissociation (HCD) fragmentation on selected precursor ions were performed in parallel with full-scan FTMS. Using HCD MS/MS, most precursor and product ion masses were determined within 1 ppm of the theoretical m/z values throughout the mass spectral range and this enhanced the reliability of analyte identity.For the analysis of mussels (M. galloprovincialis), the method limit of quantitation (LOQ) was 0.010 µg/g using full-scan FTMS and this was comparable with the LOQ (0.007 µg/g) using CID MS/MS. The repeatability data were; intra-day RSD% (1.8-4.4%; n = 6) and inter-day RSD% (4.7-8.6%; n = 3). Application of these methods to the analysis of mussels (M. edulis) that were naturally contaminated with azaspiracids, using high-resolution full-scan Orbitrap MS and low-resolution CID MS/MS, produced equivalent quantitative data.
Item Type:
Article
Language:
en
MeSH:
Animals; Food Contamination; Marine Toxins; Mytilus; Reproducibility of Results; Sensitivity and Specificity; Shellfish; Spiro Compounds; Tandem Mass Spectrometry
ISSN:
1097-0231

Full metadata record

DC FieldValue Language
dc.contributor.authorSkrabáková, Zuzanaen
dc.contributor.authorO'Halloran, Johnen
dc.contributor.authorvan Pelt, Frank N A Men
dc.contributor.authorJames, Kevin Jen
dc.date.accessioned2012-01-05T12:09:27Z-
dc.date.available2012-01-05T12:09:27Z-
dc.date.issued2010-10-30-
dc.identifier.citationFood contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish. 2010, 24 (20):2966-74 Rapid Commun. Mass Spectrom.en
dc.identifier.issn1097-0231-
dc.identifier.pmid20872629-
dc.identifier.doi10.1002/rcm.4724-
dc.identifier.urihttp://hdl.handle.net/10147/200237-
dc.description.abstractThe biotoxins, azaspiracids (AZAs), from marine phytoplankton accumulate in shellfish and affect human health by causing severe gastrointestinal disturbance, diarrhea, nausea and vomiting. Specific and sensitive methods have been developed and validated for the determination of the most commonly occurring azaspiracid analogs. An LTQ Orbitrap mass spectrometer is a hybrid instrument that combines linear ion trap (LIT) mass spectrometry (MS) with high-resolution Fourier transform (FT) MS and this was exploited to perform simultaneous ultra-high-resolution full-scan MS analysis and collision-induced dissociation (CID) tandem mass spectrometry (MS/MS). Using the highest mass resolution setting (100,000 FWHM) in full-scan mode, the methodology was validated for the determination of six AZAs in mussel (Mytilus galloprovincialis) tissue extracts. Ultra-high mass resolution, together with a narrow mass tolerance window of ±2 mDa, dramatically improved detection sensitivity. In addition to employing chromatographic resolution to distinguish between the isomeric azaspiracid analogs, AZA1/AZA6 and AZA4/AZA5, higher energy collisionally induced dissociation (HCD) fragmentation on selected precursor ions were performed in parallel with full-scan FTMS. Using HCD MS/MS, most precursor and product ion masses were determined within 1 ppm of the theoretical m/z values throughout the mass spectral range and this enhanced the reliability of analyte identity.For the analysis of mussels (M. galloprovincialis), the method limit of quantitation (LOQ) was 0.010 µg/g using full-scan FTMS and this was comparable with the LOQ (0.007 µg/g) using CID MS/MS. The repeatability data were; intra-day RSD% (1.8-4.4%; n = 6) and inter-day RSD% (4.7-8.6%; n = 3). Application of these methods to the analysis of mussels (M. edulis) that were naturally contaminated with azaspiracids, using high-resolution full-scan Orbitrap MS and low-resolution CID MS/MS, produced equivalent quantitative data.-
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subject.meshAnimals-
dc.subject.meshFood Contamination-
dc.subject.meshMarine Toxins-
dc.subject.meshMytilus-
dc.subject.meshReproducibility of Results-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshShellfish-
dc.subject.meshSpiro Compounds-
dc.subject.meshTandem Mass Spectrometry-
dc.titleFood contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish.en
dc.typeArticleen
dc.contributor.departmentPROTEOBIO (Mass Spectrometry Centre), Cork Institute of Technology, Bishopstown, Cork, Ireland.en
dc.identifier.journalRapid communications in mass spectrometry : RCMen
dc.description.provinceMunster-
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