Composition, variability, and temporal stability of the intestinal microbiota of the elderly.

Hdl Handle:
http://hdl.handle.net/10147/198761
Title:
Composition, variability, and temporal stability of the intestinal microbiota of the elderly.
Authors:
Claesson, Marcus J; Cusack, Siobhán; O'Sullivan, Orla; Greene-Diniz, Rachel; de Weerd, Heleen; Flannery, Edel; Marchesi, Julian R; Falush, Daniel; Dinan, Timothy; Fitzgerald, Gerald; Stanton, Catherine; van Sinderen, Douwe; O'Connor, Michael; Harnedy, Norma; O'Connor, Kieran; Henry, Colm; O'Mahony, Denis; Fitzgerald, Anthony P; Shanahan, Fergus; Twomey, Cillian; Hill, Colin; Ross, R Paul; O'Toole, Paul W
Affiliation:
Department of Microbiology, University College, Cork, Ireland.
Citation:
Composition, variability, and temporal stability of the intestinal microbiota of the elderly. 2011, 108 Suppl 1:4586-91 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
15-Mar-2011
URI:
http://hdl.handle.net/10147/198761
DOI:
10.1073/pnas.1000097107
PubMed ID:
20571116
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063589/pdf/pnas.201000097.pdf; http://www.pnas.org/content/108/suppl.1/4586.full.pdf+html
Abstract:
Alterations in the human intestinal microbiota are linked to conditions including inflammatory bowel disease, irritable bowel syndrome, and obesity. The microbiota also undergoes substantial changes at the extremes of life, in infants and older people, the ramifications of which are still being explored. We applied pyrosequencing of over 40,000 16S rRNA gene V4 region amplicons per subject to characterize the fecal microbiota in 161 subjects aged 65 y and older and 9 younger control subjects. The microbiota of each individual subject constituted a unique profile that was separable from all others. In 68% of the individuals, the microbiota was dominated by phylum Bacteroides, with an average proportion of 57% across all 161 baseline samples. Phylum Firmicutes had an average proportion of 40%. The proportions of some phyla and genera associated with disease or health also varied dramatically, including Proteobacteria, Actinobacteria, and Faecalibacteria. The core microbiota of elderly subjects was distinct from that previously established for younger adults, with a greater proportion of Bacteroides spp. and distinct abundance patterns of Clostridium groups. Analyses of 26 fecal microbiota datasets from 3-month follow-up samples indicated that in 85% of the subjects, the microbiota composition was more like the corresponding time-0 sample than any other dataset. We conclude that the fecal microbiota of the elderly shows temporal stability over limited time in the majority of subjects but is characterized by unusual phylum proportions and extreme variability.
Item Type:
Article
Language:
en
Description:
Alterations in the human intestinal microbiota are linked to conditions including inflammatory bowel disease, irritable bowel syndrome, and obesity. The microbiota also undergoes substantial changes at the extremes of life, in infants and older people, the ramifications of which are still being explored. We applied pyrosequencing of over 40,000 16S rRNA gene V4 region amplicons per subject to characterize the fecal microbiota in 161 subjects aged 65 y and older and 9 younger control subjects. The microbiota of each individual subject constituted a unique profile that was separable from all others. In 68% of the individuals, the microbiota was dominated by phylum Bacteroides, with an average proportion of 57% across all 161 baseline samples. Phylum Firmicutes had an average proportion of 40%. The proportions of some phyla and genera associated with disease or health also varied dramatically, including Proteobacteria, Actinobacteria, and Faecalibacteria. The core microbiota of elderly subjects was distinct from that previously established for younger adults, with a greater proportion of Bacteroides spp. and distinct abundance patterns of Clostridium groups. Analyses of 26 fecal microbiota datasets from 3-month follow-up samples indicated that in 85% of the subjects, the microbiota composition was more like the corresponding time-0 sample than any other dataset. We conclude that the fecal microbiota of the elderly shows temporal stability over limited time in the majority of subjects but is characterized by unusual phylum proportions and extreme variability.
MeSH:
Age Factors; Aged; Aged, 80 and over; Bacteria; Base Sequence; Cluster Analysis; Computational Biology; Feces; Humans; Intestines; Ireland; Metagenome; Molecular Sequence Data; Phylogeny; Principal Component Analysis; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity; Statistics, Nonparametric
ISSN:
1091-6490

Full metadata record

DC FieldValue Language
dc.contributor.authorClaesson, Marcus Jen
dc.contributor.authorCusack, Siobhánen
dc.contributor.authorO'Sullivan, Orlaen
dc.contributor.authorGreene-Diniz, Rachelen
dc.contributor.authorde Weerd, Heleenen
dc.contributor.authorFlannery, Edelen
dc.contributor.authorMarchesi, Julian Ren
dc.contributor.authorFalush, Danielen
dc.contributor.authorDinan, Timothyen
dc.contributor.authorFitzgerald, Geralden
dc.contributor.authorStanton, Catherineen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorO'Connor, Michaelen
dc.contributor.authorHarnedy, Normaen
dc.contributor.authorO'Connor, Kieranen
dc.contributor.authorHenry, Colmen
dc.contributor.authorO'Mahony, Denisen
dc.contributor.authorFitzgerald, Anthony Pen
dc.contributor.authorShanahan, Fergusen
dc.contributor.authorTwomey, Cillianen
dc.contributor.authorHill, Colinen
dc.contributor.authorRoss, R Paulen
dc.contributor.authorO'Toole, Paul Wen
dc.date.accessioned2011-12-22T16:21:29Z-
dc.date.available2011-12-22T16:21:29Z-
dc.date.issued2011-03-15-
dc.identifier.citationComposition, variability, and temporal stability of the intestinal microbiota of the elderly. 2011, 108 Suppl 1:4586-91 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490-
dc.identifier.pmid20571116-
dc.identifier.doi10.1073/pnas.1000097107-
dc.identifier.urihttp://hdl.handle.net/10147/198761-
dc.descriptionAlterations in the human intestinal microbiota are linked to conditions including inflammatory bowel disease, irritable bowel syndrome, and obesity. The microbiota also undergoes substantial changes at the extremes of life, in infants and older people, the ramifications of which are still being explored. We applied pyrosequencing of over 40,000 16S rRNA gene V4 region amplicons per subject to characterize the fecal microbiota in 161 subjects aged 65 y and older and 9 younger control subjects. The microbiota of each individual subject constituted a unique profile that was separable from all others. In 68% of the individuals, the microbiota was dominated by phylum Bacteroides, with an average proportion of 57% across all 161 baseline samples. Phylum Firmicutes had an average proportion of 40%. The proportions of some phyla and genera associated with disease or health also varied dramatically, including Proteobacteria, Actinobacteria, and Faecalibacteria. The core microbiota of elderly subjects was distinct from that previously established for younger adults, with a greater proportion of Bacteroides spp. and distinct abundance patterns of Clostridium groups. Analyses of 26 fecal microbiota datasets from 3-month follow-up samples indicated that in 85% of the subjects, the microbiota composition was more like the corresponding time-0 sample than any other dataset. We conclude that the fecal microbiota of the elderly shows temporal stability over limited time in the majority of subjects but is characterized by unusual phylum proportions and extreme variability.en
dc.description.abstractAlterations in the human intestinal microbiota are linked to conditions including inflammatory bowel disease, irritable bowel syndrome, and obesity. The microbiota also undergoes substantial changes at the extremes of life, in infants and older people, the ramifications of which are still being explored. We applied pyrosequencing of over 40,000 16S rRNA gene V4 region amplicons per subject to characterize the fecal microbiota in 161 subjects aged 65 y and older and 9 younger control subjects. The microbiota of each individual subject constituted a unique profile that was separable from all others. In 68% of the individuals, the microbiota was dominated by phylum Bacteroides, with an average proportion of 57% across all 161 baseline samples. Phylum Firmicutes had an average proportion of 40%. The proportions of some phyla and genera associated with disease or health also varied dramatically, including Proteobacteria, Actinobacteria, and Faecalibacteria. The core microbiota of elderly subjects was distinct from that previously established for younger adults, with a greater proportion of Bacteroides spp. and distinct abundance patterns of Clostridium groups. Analyses of 26 fecal microbiota datasets from 3-month follow-up samples indicated that in 85% of the subjects, the microbiota composition was more like the corresponding time-0 sample than any other dataset. We conclude that the fecal microbiota of the elderly shows temporal stability over limited time in the majority of subjects but is characterized by unusual phylum proportions and extreme variability.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063589/pdf/pnas.201000097.pdfen
dc.relation.urlhttp://www.pnas.org/content/108/suppl.1/4586.full.pdf+htmlen
dc.subject.meshAge Factors-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshBacteria-
dc.subject.meshBase Sequence-
dc.subject.meshCluster Analysis-
dc.subject.meshComputational Biology-
dc.subject.meshFeces-
dc.subject.meshHumans-
dc.subject.meshIntestines-
dc.subject.meshIreland-
dc.subject.meshMetagenome-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPhylogeny-
dc.subject.meshPrincipal Component Analysis-
dc.subject.meshRNA, Ribosomal, 16S-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshSpecies Specificity-
dc.subject.meshStatistics, Nonparametric-
dc.titleComposition, variability, and temporal stability of the intestinal microbiota of the elderly.en
dc.typeArticleen
dc.contributor.departmentDepartment of Microbiology, University College, Cork, Ireland.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
dc.description.provinceMunster-

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