Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.

Hdl Handle:
http://hdl.handle.net/10147/197879
Title:
Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.
Authors:
Jones, S; Douarre, P E; O'Leary, J; Corcoran, D; O'Mahony, J; Lucey, B
Affiliation:
Department of Medical Microbiology, Cork University Hospital, Ireland.
Citation:
Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples. 2011, 68 (3):116-9 Br. J. Biomed. Sci.
Journal:
British journal of biomedical science
Issue Date:
2011
URI:
http://hdl.handle.net/10147/197879
PubMed ID:
21950202
Abstract:
Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.
Item Type:
Article
Language:
en
Description:
Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.
MeSH:
Feces; Humans; Norovirus; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction
ISSN:
0967-4845

Full metadata record

DC FieldValue Language
dc.contributor.authorJones, Sen
dc.contributor.authorDouarre, P Een
dc.contributor.authorO'Leary, Jen
dc.contributor.authorCorcoran, Den
dc.contributor.authorO'Mahony, Jen
dc.contributor.authorLucey, Ben
dc.date.accessioned2011-12-19T16:36:14Z-
dc.date.available2011-12-19T16:36:14Z-
dc.date.issued2011-
dc.identifier.citationValidation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples. 2011, 68 (3):116-9 Br. J. Biomed. Sci.en
dc.identifier.issn0967-4845-
dc.identifier.pmid21950202-
dc.identifier.urihttp://hdl.handle.net/10147/197879-
dc.descriptionNorovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.en
dc.description.abstractNorovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.-
dc.language.isoenen
dc.subject.meshFeces-
dc.subject.meshHumans-
dc.subject.meshNorovirus-
dc.subject.meshRNA, Viral-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.titleValidation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, Cork University Hospital, Ireland.en
dc.identifier.journalBritish journal of biomedical scienceen
dc.description.provinceMunster-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.