Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics

Hdl Handle:
http://hdl.handle.net/10147/196272
Title:
Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics
Authors:
Gerard, Valerie A; Maguire, Ciaran M; Bazou, Despina; Gun'ko, Yurii K
Citation:
Journal of Nanobiotechnology. 2011 Nov 10;9(1):50
Issue Date:
10-Nov-2011
URI:
http://hdl.handle.net/10147/196272
Abstract:
Abstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorGerard, Valerie A-
dc.contributor.authorMaguire, Ciaran M-
dc.contributor.authorBazou, Despina-
dc.contributor.authorGun'ko, Yurii K-
dc.date.accessioned2011-12-07T09:44:38Z-
dc.date.available2011-12-07T09:44:38Z-
dc.date.issued2011-11-10-
dc.identifierhttp://dx.doi.org/10.1186/1477-3155-9-50-
dc.identifier.citationJournal of Nanobiotechnology. 2011 Nov 10;9(1):50-
dc.identifier.urihttp://hdl.handle.net/10147/196272-
dc.description.abstractAbstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics.-
dc.titleFolic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderGerard et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2011-12-05T12:07:33Z-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.