Contact with existing adipose tissue is inductive for adipogenesis in matrigel.

Hdl Handle:
http://hdl.handle.net/10147/143785
Title:
Contact with existing adipose tissue is inductive for adipogenesis in matrigel.
Authors:
Kelly, John L; Findlay, Michael W; Knight, Kenneth R; Penington, Anthony; Thompson, Erik W; Messina, Aurora; Morrison, Wayne A
Affiliation:
Bernard O'Brien Institute of Microsurgery, Melbourne, Australia.
Citation:
Contact with existing adipose tissue is inductive for adipogenesis in matrigel. 2006, 12 (7):2041-7 Tissue Eng.
Publisher:
Mary Ann Liebert Inc
Journal:
Tissue engineering
Issue Date:
Jul-2006
URI:
http://hdl.handle.net/10147/143785
DOI:
10.1089/ten.2006.12.2041
PubMed ID:
16889532
Additional Links:
http://www.liebertonline.com/doi/abs/10.1089/ten.2006.12.2041
Abstract:
The effect of adipose tissue on inductive adipogenesis within Matrigel (BD Biosciences) was assessed by using a murine chamber model containing a vascular pedicle. Three-chamber configurations that varied in the access to an adipose tissue source were used, including sealed- and open-chamber groups that had no access and limited access, respectively, to the surrounding adipose tissue, and a sealed-chamber group in which adipose tissue was placed as an autograft. All groups showed neovascularization, but varied in the amount of adipogenesis seen in direct relation to their access to preexisting adipose tissue: open chambers showed strong adipogenesis, whereas the sealed chambers had little or no adipose tissue; adipogenesis was restored in the autograft chamber group that contained 2- to 5-mg fat autografts. These showed significantly more adipogenesis than the sealed chambers with no autograft ( p < 0.01). Autografts with 1mg of fat were capable of producing adipogenesis but did so less consistently than the larger autografts. These findings have important implications for adipose tissue engineering strategies and for understanding de novo production of adipose tissue.
Item Type:
Article
Language:
en
Description:
The effect of adipose tissue on inductive adipogenesis within Matrigel (BD Biosciences) was assessed by using a murine chamber model containing a vascular pedicle. Three-chamber configurations that varied in the access to an adipose tissue source were used, including sealed- and open-chamber groups that had no access and limited access, respectively, to the surrounding adipose tissue, and a sealed-chamber group in which adipose tissue was placed as an autograft. All groups showed neovascularization, but varied in the amount of adipogenesis seen in direct relation to their access to preexisting adipose tissue: open chambers showed strong adipogenesis, whereas the sealed chambers had little or no adipose tissue; adipogenesis was restored in the autograft chamber group that contained 2- to 5-mg fat autografts. These showed significantly more adipogenesis than the sealed chambers with no autograft ( p < 0.01). Autografts with 1mg of fat were capable of producing adipogenesis but did so less consistently than the larger autografts. These findings have important implications for adipose tissue engineering strategies and for understanding de novo production of adipose tissue.
MeSH:
Adipogenesis; Adipose Tissue; Animals; Biocompatible Materials; Cell Communication; Cell Differentiation; Collagen; Drug Combinations; Laminin; Mice; Neovascularization, Physiologic; Proteoglycans; Transplantation, Autologous
ISSN:
1076-3279

Full metadata record

DC FieldValue Language
dc.contributor.authorKelly, John Len
dc.contributor.authorFindlay, Michael Wen
dc.contributor.authorKnight, Kenneth Ren
dc.contributor.authorPenington, Anthonyen
dc.contributor.authorThompson, Erik Wen
dc.contributor.authorMessina, Auroraen
dc.contributor.authorMorrison, Wayne Aen
dc.date.accessioned2011-10-03T14:18:50Z-
dc.date.available2011-10-03T14:18:50Z-
dc.date.issued2006-07-
dc.identifier.citationContact with existing adipose tissue is inductive for adipogenesis in matrigel. 2006, 12 (7):2041-7 Tissue Eng.en
dc.identifier.issn1076-3279-
dc.identifier.pmid16889532-
dc.identifier.doi10.1089/ten.2006.12.2041-
dc.identifier.urihttp://hdl.handle.net/10147/143785-
dc.descriptionThe effect of adipose tissue on inductive adipogenesis within Matrigel (BD Biosciences) was assessed by using a murine chamber model containing a vascular pedicle. Three-chamber configurations that varied in the access to an adipose tissue source were used, including sealed- and open-chamber groups that had no access and limited access, respectively, to the surrounding adipose tissue, and a sealed-chamber group in which adipose tissue was placed as an autograft. All groups showed neovascularization, but varied in the amount of adipogenesis seen in direct relation to their access to preexisting adipose tissue: open chambers showed strong adipogenesis, whereas the sealed chambers had little or no adipose tissue; adipogenesis was restored in the autograft chamber group that contained 2- to 5-mg fat autografts. These showed significantly more adipogenesis than the sealed chambers with no autograft ( p < 0.01). Autografts with 1mg of fat were capable of producing adipogenesis but did so less consistently than the larger autografts. These findings have important implications for adipose tissue engineering strategies and for understanding de novo production of adipose tissue.en
dc.description.abstractThe effect of adipose tissue on inductive adipogenesis within Matrigel (BD Biosciences) was assessed by using a murine chamber model containing a vascular pedicle. Three-chamber configurations that varied in the access to an adipose tissue source were used, including sealed- and open-chamber groups that had no access and limited access, respectively, to the surrounding adipose tissue, and a sealed-chamber group in which adipose tissue was placed as an autograft. All groups showed neovascularization, but varied in the amount of adipogenesis seen in direct relation to their access to preexisting adipose tissue: open chambers showed strong adipogenesis, whereas the sealed chambers had little or no adipose tissue; adipogenesis was restored in the autograft chamber group that contained 2- to 5-mg fat autografts. These showed significantly more adipogenesis than the sealed chambers with no autograft ( p < 0.01). Autografts with 1mg of fat were capable of producing adipogenesis but did so less consistently than the larger autografts. These findings have important implications for adipose tissue engineering strategies and for understanding de novo production of adipose tissue.-
dc.language.isoenen
dc.publisherMary Ann Liebert Incen
dc.relation.urlhttp://www.liebertonline.com/doi/abs/10.1089/ten.2006.12.2041en
dc.subject.meshAdipogenesis-
dc.subject.meshAdipose Tissue-
dc.subject.meshAnimals-
dc.subject.meshBiocompatible Materials-
dc.subject.meshCell Communication-
dc.subject.meshCell Differentiation-
dc.subject.meshCollagen-
dc.subject.meshDrug Combinations-
dc.subject.meshLaminin-
dc.subject.meshMice-
dc.subject.meshNeovascularization, Physiologic-
dc.subject.meshProteoglycans-
dc.subject.meshTransplantation, Autologous-
dc.titleContact with existing adipose tissue is inductive for adipogenesis in matrigel.en
dc.typeArticleen
dc.contributor.departmentBernard O'Brien Institute of Microsurgery, Melbourne, Australia.en
dc.identifier.journalTissue engineeringen
dc.description.provinceConnacht-

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