Sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype

Hdl Handle:
http://hdl.handle.net/10147/142630
Title:
Sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype
Authors:
Meleady, Paula; Doolan, Padraig; Henry, Michael; Barron, Niall; Keenan, Joanne; O'Sullivan, Finbar; Clarke, Colin; Gammell, Patrick; Melville, Mark W; Leonard, Mark; Clynes, Martin
Citation:
BMC Biotechnology. 2011 Jul 24;11(1):78
Issue Date:
24-Jul-2011
URI:
http://hdl.handle.net/10147/142630
Abstract:
Abstract Background The ability of mammalian cell lines to sustain cell specific productivity (Qp) over the full duration of bioprocess culture is a highly desirable phenotype, but the molecular basis for sustainable productivity has not been previously investigated in detail. In order to identify proteins that may be associated with a sustained productivity phenotype, we have conducted a proteomic profiling analysis of two matched pairs of monoclonal antibody-producing Chinese hamster ovary (CHO) cell lines that differ in their ability to sustain productivity over a 10 day fed-batch culture. Results Proteomic profiling of inherent differences between the two sets of comparators using 2D-DIGE (Difference Gel Electrophoresis) and LC-MS/MS resulted in the identification of 89 distinct differentially expressed proteins. Overlap comparisons between the two sets of cell line pairs identified 12 proteins (AKRIB8, ANXA1, ANXA4, EIF3I, G6PD, HSPA8, HSP90B1, HSPD1, NUDC, PGAM1, RUVBL1 and CNN3) that were differentially expressed in the same direction. Conclusion These proteins may have an important role in sustaining high productivity of recombinant protein over the duration of a fed-batch bioprocess culture. It is possible that many of these proteins could be useful for future approaches to successfully manipulate or engineer CHO cells in order to sustain productivity of recombinant protein.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorMeleady, Paula-
dc.contributor.authorDoolan, Padraig-
dc.contributor.authorHenry, Michael-
dc.contributor.authorBarron, Niall-
dc.contributor.authorKeenan, Joanne-
dc.contributor.authorO'Sullivan, Finbar-
dc.contributor.authorClarke, Colin-
dc.contributor.authorGammell, Patrick-
dc.contributor.authorMelville, Mark W-
dc.contributor.authorLeonard, Mark-
dc.contributor.authorClynes, Martin-
dc.date.accessioned2011-09-19T14:27:48Z-
dc.date.available2011-09-19T14:27:48Z-
dc.date.issued2011-07-24-
dc.identifierhttp://dx.doi.org/10.1186/1472-6750-11-78-
dc.identifier.citationBMC Biotechnology. 2011 Jul 24;11(1):78-
dc.identifier.urihttp://hdl.handle.net/10147/142630-
dc.description.abstractAbstract Background The ability of mammalian cell lines to sustain cell specific productivity (Qp) over the full duration of bioprocess culture is a highly desirable phenotype, but the molecular basis for sustainable productivity has not been previously investigated in detail. In order to identify proteins that may be associated with a sustained productivity phenotype, we have conducted a proteomic profiling analysis of two matched pairs of monoclonal antibody-producing Chinese hamster ovary (CHO) cell lines that differ in their ability to sustain productivity over a 10 day fed-batch culture. Results Proteomic profiling of inherent differences between the two sets of comparators using 2D-DIGE (Difference Gel Electrophoresis) and LC-MS/MS resulted in the identification of 89 distinct differentially expressed proteins. Overlap comparisons between the two sets of cell line pairs identified 12 proteins (AKRIB8, ANXA1, ANXA4, EIF3I, G6PD, HSPA8, HSP90B1, HSPD1, NUDC, PGAM1, RUVBL1 and CNN3) that were differentially expressed in the same direction. Conclusion These proteins may have an important role in sustaining high productivity of recombinant protein over the duration of a fed-batch bioprocess culture. It is possible that many of these proteins could be useful for future approaches to successfully manipulate or engineer CHO cells in order to sustain productivity of recombinant protein.-
dc.titleSustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderMeleady et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2011-09-12T15:43:21Z-
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