The sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue.

Hdl Handle:
http://hdl.handle.net/10147/141047
Title:
The sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue.
Authors:
Ryan, James; Curran, Catherine E; Hennessy, Emer; Newell, John; Morris, John C; Kerin, Michael J; Dwyer, Roisin M
Affiliation:
Division of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Citation:
The sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue. 2011, 6 (1):e16023 PLoS ONE
Journal:
PloS one
Issue Date:
Jan-2011
URI:
http://hdl.handle.net/10147/141047
DOI:
10.1371/journal.pone.0016023
PubMed ID:
21283523
Abstract:
The presence, relevance and regulation of the Sodium Iodide Symporter (NIS) in human mammary tissue remains poorly understood. This study aimed to quantify relative expression of NIS and putative regulators in human breast tissue, with relationships observed further investigated in vitro.; Human breast tissue specimens (malignant n = 75, normal n = 15, fibroadenoma n = 10) were analysed by RQ-PCR targeting NIS, receptors for retinoic acid (RARα, RARβ), oestrogen (ERα), thyroid hormones (THRα, THRβ), and also phosphoinositide-3-kinase (PI3K). Breast cancer cells were treated with Retinoic acid (ATRA), Estradiol and Thyroxine individually and in combination followed by analysis of changes in NIS expression.; The lowest levels of NIS were detected in normal tissue (Mean(SEM) 0.70(0.12) Log(10) Relative Quantity (RQ)) with significantly higher levels observed in fibroadenoma (1.69(0.21) Log(10)RQ, p<0.005) and malignant breast tissue (1.18(0.07) Log(10)RQ, p<0.05). Significant positive correlations were observed between human NIS and ERα (r = 0.22, p<0.05) and RARα (r = 0.29, p<0.005), with the strongest relationship observed between NIS and RARβ (r = 0.38, p<0.0001). An inverse relationship between NIS and PI3K expression was also observed (r =  0.21, p<0.05). In vitro, ATRA, Estradiol and Thyroxine individually stimulated significant increases in NIS expression (range 6-16 fold), while ATRA and Thyroxine combined caused the greatest increase (range 16-26 fold).; Although NIS expression is significantly higher in malignant compared to normal breast tissue, the highest level was detected in fibroadenoma. The data presented supports a role for retinoic acid and estradiol in mammary NIS regulation in vivo, and also highlights potential thyroidal regulation of mammary NIS mediated by thyroid hormones.
Item Type:
Article
Language:
en
MeSH:
Breast Neoplasms; Estradiol; Female; Fibroadenoma; Gene Expression Regulation, Neoplastic; Humans; Mammary Glands, Human; Symporters; Thyroid Hormones; Thyroxine; Tretinoin
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorRyan, Jamesen
dc.contributor.authorCurran, Catherine Een
dc.contributor.authorHennessy, Emeren
dc.contributor.authorNewell, Johnen
dc.contributor.authorMorris, John Cen
dc.contributor.authorKerin, Michael Jen
dc.contributor.authorDwyer, Roisin Men
dc.date.accessioned2011-08-29T10:09:01Z-
dc.date.available2011-08-29T10:09:01Z-
dc.date.issued2011-01-
dc.identifier.citationThe sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue. 2011, 6 (1):e16023 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21283523-
dc.identifier.doi10.1371/journal.pone.0016023-
dc.identifier.urihttp://hdl.handle.net/10147/141047-
dc.description.abstractThe presence, relevance and regulation of the Sodium Iodide Symporter (NIS) in human mammary tissue remains poorly understood. This study aimed to quantify relative expression of NIS and putative regulators in human breast tissue, with relationships observed further investigated in vitro.-
dc.description.abstractHuman breast tissue specimens (malignant n = 75, normal n = 15, fibroadenoma n = 10) were analysed by RQ-PCR targeting NIS, receptors for retinoic acid (RARα, RARβ), oestrogen (ERα), thyroid hormones (THRα, THRβ), and also phosphoinositide-3-kinase (PI3K). Breast cancer cells were treated with Retinoic acid (ATRA), Estradiol and Thyroxine individually and in combination followed by analysis of changes in NIS expression.-
dc.description.abstractThe lowest levels of NIS were detected in normal tissue (Mean(SEM) 0.70(0.12) Log(10) Relative Quantity (RQ)) with significantly higher levels observed in fibroadenoma (1.69(0.21) Log(10)RQ, p<0.005) and malignant breast tissue (1.18(0.07) Log(10)RQ, p<0.05). Significant positive correlations were observed between human NIS and ERα (r = 0.22, p<0.05) and RARα (r = 0.29, p<0.005), with the strongest relationship observed between NIS and RARβ (r = 0.38, p<0.0001). An inverse relationship between NIS and PI3K expression was also observed (r =  0.21, p<0.05). In vitro, ATRA, Estradiol and Thyroxine individually stimulated significant increases in NIS expression (range 6-16 fold), while ATRA and Thyroxine combined caused the greatest increase (range 16-26 fold).-
dc.description.abstractAlthough NIS expression is significantly higher in malignant compared to normal breast tissue, the highest level was detected in fibroadenoma. The data presented supports a role for retinoic acid and estradiol in mammary NIS regulation in vivo, and also highlights potential thyroidal regulation of mammary NIS mediated by thyroid hormones.-
dc.language.isoenen
dc.subject.meshBreast Neoplasms-
dc.subject.meshEstradiol-
dc.subject.meshFemale-
dc.subject.meshFibroadenoma-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshHumans-
dc.subject.meshMammary Glands, Human-
dc.subject.meshSymporters-
dc.subject.meshThyroid Hormones-
dc.subject.meshThyroxine-
dc.subject.meshTretinoin-
dc.titleThe sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue.en
dc.typeArticleen
dc.contributor.departmentDivision of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.en
dc.identifier.journalPloS oneen
dc.description.provinceConnacht-

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