Rapid effects of 17beta-estradiol on epithelial TRPV6 Ca2+ channel in human T84 colonic cells.

Hdl Handle:
http://hdl.handle.net/10147/136784
Title:
Rapid effects of 17beta-estradiol on epithelial TRPV6 Ca2+ channel in human T84 colonic cells.
Authors:
Irnaten, Mustapha; Blanchard-Gutton, Nicolas; Harvey, Brian J
Affiliation:
Molecular Medicine Laboratories, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland. mirnaten@rcsi.ie
Citation:
Rapid effects of 17beta-estradiol on epithelial TRPV6 Ca2+ channel in human T84 colonic cells. 2008, 44 (5):441-52 Cell Calcium
Journal:
Cell calcium
Issue Date:
Nov-2008
URI:
http://hdl.handle.net/10147/136784
DOI:
10.1016/j.ceca.2008.02.007
PubMed ID:
18395250
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18395250
Abstract:
The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of whole body Ca(2+) homeostasis. The role of steroid hormone 17beta-estradiol (E(2)), in [Ca(2+)](i) regulation involving TRPV6 has been only limited at the protein expression levels in over-expressing heterologous systems. In the present study, using a combination of calcium-imaging, whole-cell patch-clamp techniques and siRNA technology to specifically knockdown TRPV6 protein expression, we were able to (i) show that TRPV6 is natively, rather than exogenously, expressed at mRNA and protein levels in human T84 colonic cells, (ii) characterize functional TRPV6 channels and (iii) demonstrate, for the first time, the rapid effects of E(2) in [Ca(2+)](i) regulation involving directly TRPV6 channels in T84 cells. Treatment with E(2) rapidly (<5 min) enhanced [Ca(2+)](i) and this increase was partially but significantly prevented when cells were pre-treated with ruthenium red and completely abolished in cells treated with siRNA specifically targeting TRPV6 protein expression. These results indicate that when cells are stimulated by E(2), Ca(2+) enters the cell through TRPV6 channels. TRPV6 channels in T84 cells contribute to the Ca(2+) entry/signalling pathway that is sensitive to 17beta-estradiol.
Item Type:
Article
Language:
en
MeSH:
Calcium; Calcium Channels; Cell Line; Colon; Estradiol; Humans; Patch-Clamp Techniques; RNA, Small Interfering; Signal Transduction; TRPV Cation Channels
ISSN:
0143-4160

Full metadata record

DC FieldValue Language
dc.contributor.authorIrnaten, Mustaphaen
dc.contributor.authorBlanchard-Gutton, Nicolasen
dc.contributor.authorHarvey, Brian Jen
dc.date.accessioned2011-07-25T08:37:19Z-
dc.date.available2011-07-25T08:37:19Z-
dc.date.issued2008-11-
dc.identifier.citationRapid effects of 17beta-estradiol on epithelial TRPV6 Ca2+ channel in human T84 colonic cells. 2008, 44 (5):441-52 Cell Calciumen
dc.identifier.issn0143-4160-
dc.identifier.pmid18395250-
dc.identifier.doi10.1016/j.ceca.2008.02.007-
dc.identifier.urihttp://hdl.handle.net/10147/136784-
dc.description.abstractThe control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of whole body Ca(2+) homeostasis. The role of steroid hormone 17beta-estradiol (E(2)), in [Ca(2+)](i) regulation involving TRPV6 has been only limited at the protein expression levels in over-expressing heterologous systems. In the present study, using a combination of calcium-imaging, whole-cell patch-clamp techniques and siRNA technology to specifically knockdown TRPV6 protein expression, we were able to (i) show that TRPV6 is natively, rather than exogenously, expressed at mRNA and protein levels in human T84 colonic cells, (ii) characterize functional TRPV6 channels and (iii) demonstrate, for the first time, the rapid effects of E(2) in [Ca(2+)](i) regulation involving directly TRPV6 channels in T84 cells. Treatment with E(2) rapidly (<5 min) enhanced [Ca(2+)](i) and this increase was partially but significantly prevented when cells were pre-treated with ruthenium red and completely abolished in cells treated with siRNA specifically targeting TRPV6 protein expression. These results indicate that when cells are stimulated by E(2), Ca(2+) enters the cell through TRPV6 channels. TRPV6 channels in T84 cells contribute to the Ca(2+) entry/signalling pathway that is sensitive to 17beta-estradiol.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18395250en
dc.subject.meshCalcium-
dc.subject.meshCalcium Channels-
dc.subject.meshCell Line-
dc.subject.meshColon-
dc.subject.meshEstradiol-
dc.subject.meshHumans-
dc.subject.meshPatch-Clamp Techniques-
dc.subject.meshRNA, Small Interfering-
dc.subject.meshSignal Transduction-
dc.subject.meshTRPV Cation Channels-
dc.titleRapid effects of 17beta-estradiol on epithelial TRPV6 Ca2+ channel in human T84 colonic cells.en
dc.typeArticleen
dc.contributor.departmentMolecular Medicine Laboratories, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland. mirnaten@rcsi.ieen
dc.identifier.journalCell calciumen
dc.description.provinceLeinster-

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