Role of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression.

Hdl Handle:
http://hdl.handle.net/10147/136778
Title:
Role of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression.
Authors:
Mackle, T; Gendy, S S; Walsh, M; McConn-Walsh, R; Costello, R W; Walsh, M-T
Affiliation:
Department of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland. Mackle_tara@hotmail.com
Citation:
Role of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression. 2008, 122 (12):1309-17 J Laryngol Otol
Journal:
The Journal of laryngology and otology
Issue Date:
Dec-2008
URI:
http://hdl.handle.net/10147/136778
DOI:
10.1017/S0022215108003241
PubMed ID:
18808729
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18808729
Abstract:
Recent research has indicated that sphingosine 1-phosphate plays a role in allergy. This study examined the effect of allergen challenge on the expression of sphingosine 1-phosphate receptors on the eosinophils of allergic rhinitis patients, and the effect of steroid treatment on this expression.; A prospective, non-randomised study.; The study had three parts. Firstly, sphingosine 1-phosphate receptor expression on the eosinophils of allergic rhinitis patients and control patients was determined. Secondly, sphingosine 1-phosphate receptor expression was quantified pre- and post-allergen challenge, before and after a short course of fluticasone propionate; all patients underwent symptom scoring and peak nasal inspiratory flow measurement pre- and post-allergen challenge, both before and after steroid or saline treatment. Thirdly, the effect of sphingosine 1-phosphate on eosinophil migration was examined.; The eosinophils of both allergic rhinitis patients and controls expressed sphingosine 1-phosphate1, 3, 4, and 5. Eosinophils from all allergic rhinitis patients demonstrated up-regulation in sphingosine 1-phosphate expression after allergen challenge. These changes were statistically very significant for sphingosine 1-phosphate1, 4, and 5, and moderately significant for sphingosine 1-phosphate3. Sphingosine 1-phosphate receptor expression up-regulation was abolished in the steroid-treated group after allergen challenge; however, the saline-treated group showed no change in sphingosine 1-phosphate receptor expression after allergen challenge. Peak nasal inspiratory flow scores were significantly diminished after allergen challenge prior to treatment, but not after a course of topical nasal steroids. Sphingosine 1-phosphate induced eosinophil chemotaxis was increased following allergen challenge in allergic rhinitis subjects.; Local intranasal steroid therapy acts directly to block allergen-induced up-regulation of sphingosine 1-phosphate receptors on the peripheral eosinophils of allergic rhinitis patients, and this is coincident with post-challenge peak nasal inspiratory flow measurement improvements. These observations support the idea that such an increase in sphingosine 1-phosphate receptor expression is clinically relevant in allergic rhinitis, with potential consequences for eosinophil migration and survival.
Item Type:
Article
Language:
en
MeSH:
Administration, Topical; Adult; Androstadienes; Cell Movement; Cells, Cultured; Eosinophils; Female; Gene Expression; Humans; Male; Middle Aged; Nasal Provocation Tests; Prospective Studies; Receptors, Lysosphingolipid; Reverse Transcriptase Polymerase Chain Reaction; Rhinitis, Allergic, Perennial; Steroids; Up-Regulation; Young Adult
ISSN:
1748-5460

Full metadata record

DC FieldValue Language
dc.contributor.authorMackle, Ten
dc.contributor.authorGendy, S Sen
dc.contributor.authorWalsh, Men
dc.contributor.authorMcConn-Walsh, Ren
dc.contributor.authorCostello, R Wen
dc.contributor.authorWalsh, M-Ten
dc.date.accessioned2011-07-25T08:34:12Z-
dc.date.available2011-07-25T08:34:12Z-
dc.date.issued2008-12-
dc.identifier.citationRole of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression. 2008, 122 (12):1309-17 J Laryngol Otolen
dc.identifier.issn1748-5460-
dc.identifier.pmid18808729-
dc.identifier.doi10.1017/S0022215108003241-
dc.identifier.urihttp://hdl.handle.net/10147/136778-
dc.description.abstractRecent research has indicated that sphingosine 1-phosphate plays a role in allergy. This study examined the effect of allergen challenge on the expression of sphingosine 1-phosphate receptors on the eosinophils of allergic rhinitis patients, and the effect of steroid treatment on this expression.-
dc.description.abstractA prospective, non-randomised study.-
dc.description.abstractThe study had three parts. Firstly, sphingosine 1-phosphate receptor expression on the eosinophils of allergic rhinitis patients and control patients was determined. Secondly, sphingosine 1-phosphate receptor expression was quantified pre- and post-allergen challenge, before and after a short course of fluticasone propionate; all patients underwent symptom scoring and peak nasal inspiratory flow measurement pre- and post-allergen challenge, both before and after steroid or saline treatment. Thirdly, the effect of sphingosine 1-phosphate on eosinophil migration was examined.-
dc.description.abstractThe eosinophils of both allergic rhinitis patients and controls expressed sphingosine 1-phosphate1, 3, 4, and 5. Eosinophils from all allergic rhinitis patients demonstrated up-regulation in sphingosine 1-phosphate expression after allergen challenge. These changes were statistically very significant for sphingosine 1-phosphate1, 4, and 5, and moderately significant for sphingosine 1-phosphate3. Sphingosine 1-phosphate receptor expression up-regulation was abolished in the steroid-treated group after allergen challenge; however, the saline-treated group showed no change in sphingosine 1-phosphate receptor expression after allergen challenge. Peak nasal inspiratory flow scores were significantly diminished after allergen challenge prior to treatment, but not after a course of topical nasal steroids. Sphingosine 1-phosphate induced eosinophil chemotaxis was increased following allergen challenge in allergic rhinitis subjects.-
dc.description.abstractLocal intranasal steroid therapy acts directly to block allergen-induced up-regulation of sphingosine 1-phosphate receptors on the peripheral eosinophils of allergic rhinitis patients, and this is coincident with post-challenge peak nasal inspiratory flow measurement improvements. These observations support the idea that such an increase in sphingosine 1-phosphate receptor expression is clinically relevant in allergic rhinitis, with potential consequences for eosinophil migration and survival.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18808729en
dc.subject.meshAdministration, Topical-
dc.subject.meshAdult-
dc.subject.meshAndrostadienes-
dc.subject.meshCell Movement-
dc.subject.meshCells, Cultured-
dc.subject.meshEosinophils-
dc.subject.meshFemale-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNasal Provocation Tests-
dc.subject.meshProspective Studies-
dc.subject.meshReceptors, Lysosphingolipid-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshRhinitis, Allergic, Perennial-
dc.subject.meshSteroids-
dc.subject.meshUp-Regulation-
dc.subject.meshYoung Adult-
dc.titleRole of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression.en
dc.typeArticleen
dc.contributor.departmentDepartment of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland. Mackle_tara@hotmail.comen
dc.identifier.journalThe Journal of laryngology and otologyen
dc.description.provinceLeinster-
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