Molecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene.

Hdl Handle:
http://hdl.handle.net/10147/136400
Title:
Molecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene.
Authors:
Walsh, A; Rourke, F O; Crowley, B
Affiliation:
Microbiology Department, St. Jame's Hospital, James Street, Dublin 8, Ireland. adwalsh@stjames.ie
Citation:
Molecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene. 2011, 30 (4):561-7 Eur. J. Clin. Microbiol. Infect. Dis.
Journal:
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10147/136400
DOI:
10.1007/s10096-010-1120-y
PubMed ID:
21170565
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21170565
Abstract:
Culture for detection of Neisseria gonorrhoeae (NG) is being replaced by molecular assays, but difficulties are observed with false positive and negatives results, especially for extragenital samples. This study evaluates the Abbott CT/NG Real-Time assay and a real-time porA pseudogene assay. Samples (n = 600) from a mixed prevalence Irish population include 164 male urines with corresponding urethral swabs, 58 endocervical swabs, 173 male pharyngeal swabs, 205 male rectal swabs, 36 NG clinical isolates and 26 commensal Neisseria species isolates. There was a 100% concordance between the Abbott CT/NG Real-Time and the porA assay. The positivity rate was 1.2%, 1.7%, 8.1% and 5.8% for FVU/urethral swabs, endocervical, pharyngeal and rectal swabs, respectively. These results were compared to culture and discrepancies were found with nine pharyngeal and three rectal swabs. Seven of the 12 discrepant positive samples were sequenced and were confirmed "true positives". The sensitivity and specificity of the molecular assays was 100%. The sensitivity of the culture-based testing was 100% for urogenital samples but 36% and 75% for pharyngeal and rectal swabs, respectively. The combined Abbott CT/NG and porA assays provide a valuable alternative to culture and also generate a significant increase in the diagnosis of pharyngeal and rectal NG infection.
Item Type:
Article
Language:
en
MeSH:
Culture Media; DNA, Bacterial; Female; Female Urogenital Diseases; Gonorrhea; Humans; Male; Male Urogenital Diseases; Neisseria gonorrhoeae; Nucleic Acid Amplification Techniques; Pharyngeal Diseases; Pharynx; Porins; Prevalence; Pseudogenes; Reagent Kits, Diagnostic; Rectal Diseases; Rectum; Sensitivity and Specificity
ISSN:
1435-4373

Full metadata record

DC FieldValue Language
dc.contributor.authorWalsh, Aen
dc.contributor.authorRourke, F Oen
dc.contributor.authorCrowley, Ben
dc.date.accessioned2011-07-20T11:26:11Z-
dc.date.available2011-07-20T11:26:11Z-
dc.date.issued2011-04-
dc.identifier.citationMolecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene. 2011, 30 (4):561-7 Eur. J. Clin. Microbiol. Infect. Dis.en
dc.identifier.issn1435-4373-
dc.identifier.pmid21170565-
dc.identifier.doi10.1007/s10096-010-1120-y-
dc.identifier.urihttp://hdl.handle.net/10147/136400-
dc.description.abstractCulture for detection of Neisseria gonorrhoeae (NG) is being replaced by molecular assays, but difficulties are observed with false positive and negatives results, especially for extragenital samples. This study evaluates the Abbott CT/NG Real-Time assay and a real-time porA pseudogene assay. Samples (n = 600) from a mixed prevalence Irish population include 164 male urines with corresponding urethral swabs, 58 endocervical swabs, 173 male pharyngeal swabs, 205 male rectal swabs, 36 NG clinical isolates and 26 commensal Neisseria species isolates. There was a 100% concordance between the Abbott CT/NG Real-Time and the porA assay. The positivity rate was 1.2%, 1.7%, 8.1% and 5.8% for FVU/urethral swabs, endocervical, pharyngeal and rectal swabs, respectively. These results were compared to culture and discrepancies were found with nine pharyngeal and three rectal swabs. Seven of the 12 discrepant positive samples were sequenced and were confirmed "true positives". The sensitivity and specificity of the molecular assays was 100%. The sensitivity of the culture-based testing was 100% for urogenital samples but 36% and 75% for pharyngeal and rectal swabs, respectively. The combined Abbott CT/NG and porA assays provide a valuable alternative to culture and also generate a significant increase in the diagnosis of pharyngeal and rectal NG infection.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21170565en
dc.subject.meshCulture Media-
dc.subject.meshDNA, Bacterial-
dc.subject.meshFemale-
dc.subject.meshFemale Urogenital Diseases-
dc.subject.meshGonorrhea-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMale Urogenital Diseases-
dc.subject.meshNeisseria gonorrhoeae-
dc.subject.meshNucleic Acid Amplification Techniques-
dc.subject.meshPharyngeal Diseases-
dc.subject.meshPharynx-
dc.subject.meshPorins-
dc.subject.meshPrevalence-
dc.subject.meshPseudogenes-
dc.subject.meshReagent Kits, Diagnostic-
dc.subject.meshRectal Diseases-
dc.subject.meshRectum-
dc.subject.meshSensitivity and Specificity-
dc.titleMolecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene.en
dc.typeArticleen
dc.contributor.departmentMicrobiology Department, St. Jame's Hospital, James Street, Dublin 8, Ireland. adwalsh@stjames.ieen
dc.identifier.journalEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiologyen
dc.description.provinceLeinster-

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