Analysis of APC allelic imbalance/loss of heterozygosity and APC protein expression in cutaneous squamous cell carcinomas.

Hdl Handle:
http://hdl.handle.net/10147/136398
Title:
Analysis of APC allelic imbalance/loss of heterozygosity and APC protein expression in cutaneous squamous cell carcinomas.
Authors:
Gray, Sarah E; Kay, Elaine W; Leader, Mary; Mabruk, Mohamed
Affiliation:
Pathology Department, Royal College of Surgeons in Ireland and Beaumont Hospital, Dublin, Ireland.
Citation:
Analysis of APC allelic imbalance/loss of heterozygosity and APC protein expression in cutaneous squamous cell carcinomas., 8 (3):149-55 Cancer Genomics Proteomics
Journal:
Cancer genomics & proteomics
Issue Date:
May-2011
URI:
http://hdl.handle.net/10147/136398
PubMed ID:
21518821
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21518821
Abstract:
The adenomatous polyposis coli (APC) gene is a tumor suppressor gene which is mutated in the hereditary disease, familial adenomatous polyposis (FAP). Somatic mutations of the APC gene have also been identified in the majority of sporadic colorectal carcinomas, and mutation of the APC gene appears to be an early step in the initiation of colon cancer. Loss of heterozygosity (LOH) of APC has been described in a variety of other cancer types, including renal cell carcinoma, gastric cancer, non-small cell lung cancer, endometrial cancer and oral squamous cell carcinomas (SCC).; To determine the role played by APC gene in the genesis of cutaneous SCC.; Allelic imbalance/loss of heterozygosity (AI/LOH) was examined in twenty-two histologically confirmed cutaneous squamous cell carcinomas (SCC) using microsatellite markers, proximal to the APC gene. Immunohistochemical analysis of APC protein expression was also examined in the cutaneous SCC.; AI/LOH was detected in 60% of the SCC samples using D5S346 marker (proximal to the APC gene). Ninty-five percent of the SCC samples showed positive reduced APC expression, however the localization of the APC protein was abnormal.; The abnormal expression of APC suggests that APC gene may play a role in cutaneous SCC development.
Item Type:
Article
Language:
en
ISSN:
1790-6295

Full metadata record

DC FieldValue Language
dc.contributor.authorGray, Sarah Een
dc.contributor.authorKay, Elaine Wen
dc.contributor.authorLeader, Maryen
dc.contributor.authorMabruk, Mohameden
dc.date.accessioned2011-07-20T10:07:35Z-
dc.date.available2011-07-20T10:07:35Z-
dc.date.issued2011-05-
dc.identifier.citationAnalysis of APC allelic imbalance/loss of heterozygosity and APC protein expression in cutaneous squamous cell carcinomas., 8 (3):149-55 Cancer Genomics Proteomicsen
dc.identifier.issn1790-6295-
dc.identifier.pmid21518821-
dc.identifier.urihttp://hdl.handle.net/10147/136398-
dc.description.abstractThe adenomatous polyposis coli (APC) gene is a tumor suppressor gene which is mutated in the hereditary disease, familial adenomatous polyposis (FAP). Somatic mutations of the APC gene have also been identified in the majority of sporadic colorectal carcinomas, and mutation of the APC gene appears to be an early step in the initiation of colon cancer. Loss of heterozygosity (LOH) of APC has been described in a variety of other cancer types, including renal cell carcinoma, gastric cancer, non-small cell lung cancer, endometrial cancer and oral squamous cell carcinomas (SCC).-
dc.description.abstractTo determine the role played by APC gene in the genesis of cutaneous SCC.-
dc.description.abstractAllelic imbalance/loss of heterozygosity (AI/LOH) was examined in twenty-two histologically confirmed cutaneous squamous cell carcinomas (SCC) using microsatellite markers, proximal to the APC gene. Immunohistochemical analysis of APC protein expression was also examined in the cutaneous SCC.-
dc.description.abstractAI/LOH was detected in 60% of the SCC samples using D5S346 marker (proximal to the APC gene). Ninty-five percent of the SCC samples showed positive reduced APC expression, however the localization of the APC protein was abnormal.-
dc.description.abstractThe abnormal expression of APC suggests that APC gene may play a role in cutaneous SCC development.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21518821en
dc.titleAnalysis of APC allelic imbalance/loss of heterozygosity and APC protein expression in cutaneous squamous cell carcinomas.en
dc.typeArticleen
dc.contributor.departmentPathology Department, Royal College of Surgeons in Ireland and Beaumont Hospital, Dublin, Ireland.en
dc.identifier.journalCancer genomics & proteomicsen
dc.description.provinceLeinster-
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