Preferential Th1 cytokine profile of phosphoantigen-stimulated human Vγ9Vδ2 T cells.

Hdl Handle:
http://hdl.handle.net/10147/135973
Title:
Preferential Th1 cytokine profile of phosphoantigen-stimulated human Vγ9Vδ2 T cells.
Authors:
Dunne, Margaret R; Mangan, Bozgana A; Madrigal-Estebas, Laura; Doherty, Derek G
Affiliation:
Department of Immunology and Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
Citation:
Preferential Th1 cytokine profile of phosphoantigen-stimulated human Vγ9Vδ2 T cells. 2010, 2010:704941 Mediators Inflamm.
Journal:
Mediators of inflammation
Issue Date:
2010
URI:
http://hdl.handle.net/10147/135973
DOI:
10.1155/2010/704941
PubMed ID:
21403900
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21403900
Abstract:
Human Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15. We show that fresh Vγ9Vδ2 T cells produce interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these conditions. Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8. Addition of IL-2 or IL-15 caused expansion of IFN-γ-producing Vγ9Vδ2 T cells, but did not enhance IFN-γ secretion after 24-72 hours. Thus, phosphoantigen-stimulated Vγ9Vδ2 T cells have potential as Th1-biasing adjuvants for immunotherapy.
Item Type:
Article
Language:
en
MeSH:
Antigens; Cytokines; Diphosphates; Hemiterpenes; Humans; Immunity, Innate; Interferon-gamma; Interleukins; Organophosphorus Compounds; Receptors, Antigen, T-Cell, gamma-delta; T-Lymphocyte Subsets; Th1 Cells; Tumor Necrosis Factor-alpha
ISSN:
1466-1861

Full metadata record

DC FieldValue Language
dc.contributor.authorDunne, Margaret Ren
dc.contributor.authorMangan, Bozgana Aen
dc.contributor.authorMadrigal-Estebas, Lauraen
dc.contributor.authorDoherty, Derek Gen
dc.date.accessioned2011-07-13T12:10:03Z-
dc.date.available2011-07-13T12:10:03Z-
dc.date.issued2010-
dc.identifier.citationPreferential Th1 cytokine profile of phosphoantigen-stimulated human Vγ9Vδ2 T cells. 2010, 2010:704941 Mediators Inflamm.en
dc.identifier.issn1466-1861-
dc.identifier.pmid21403900-
dc.identifier.doi10.1155/2010/704941-
dc.identifier.urihttp://hdl.handle.net/10147/135973-
dc.description.abstractHuman Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15. We show that fresh Vγ9Vδ2 T cells produce interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these conditions. Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8. Addition of IL-2 or IL-15 caused expansion of IFN-γ-producing Vγ9Vδ2 T cells, but did not enhance IFN-γ secretion after 24-72 hours. Thus, phosphoantigen-stimulated Vγ9Vδ2 T cells have potential as Th1-biasing adjuvants for immunotherapy.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21403900en
dc.subject.meshAntigens-
dc.subject.meshCytokines-
dc.subject.meshDiphosphates-
dc.subject.meshHemiterpenes-
dc.subject.meshHumans-
dc.subject.meshImmunity, Innate-
dc.subject.meshInterferon-gamma-
dc.subject.meshInterleukins-
dc.subject.meshOrganophosphorus Compounds-
dc.subject.meshReceptors, Antigen, T-Cell, gamma-delta-
dc.subject.meshT-Lymphocyte Subsets-
dc.subject.meshTh1 Cells-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.titlePreferential Th1 cytokine profile of phosphoantigen-stimulated human Vγ9Vδ2 T cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology and Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.en
dc.identifier.journalMediators of inflammationen
dc.description.provinceLeinster-
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