Soluble Flt-1 and PlGF: new markers of early pregnancy loss?

Hdl Handle:
http://hdl.handle.net/10147/135862
Title:
Soluble Flt-1 and PlGF: new markers of early pregnancy loss?
Authors:
Muttukrishna, Shanthi; Swer, Michelle; Suri, Sangeeta; Jamil, Amna; Calleja-Agius, Jean; Gangooly, Subrata; Ludlow, Helen; Jurkovic, Davor; Jauniaux, Eric
Affiliation:
Department of Obstetrics and Gynaecology, Anu Research Centre, University College Cork, Cork University Maternity Hospital, Wilton, Cork, Republic of Ireland. S.Muttukrishna@ucc.ie
Citation:
Soluble Flt-1 and PlGF: new markers of early pregnancy loss? 2011, 6 (3):e18041 PLoS ONE
Journal:
PloS one
Issue Date:
2011
URI:
http://hdl.handle.net/10147/135862
DOI:
10.1371/journal.pone.0018041
PubMed ID:
21448460
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21448460
Abstract:
Recent data have indicated a relationship between placental oxygen and angiogenic protein levels in the first trimester of normal pregnancies. Our objective was to investigate if maternal serum levels of angiogenic factors Soluble vascular endothelial growth factor (VEGF) receptor 1 (sFlt-1), soluble Endoglin and placental growth factor (PlGF) are altered in women with symptoms of threatened miscarriage (TM) and if they are predictive of a subsequent miscarriage. Blood samples were collected at 6-10 weeks from women presenting with TM (n = 40), from asymptomatic controls (n = 32) and from non- pregnant women in their luteal phase (n = 14). All samples were assayed for serum level of sFLT-1, PlGF, sEndoglin and HSP70 using commercial ELISAs. Samples were analysed retrospectively on the basis of pregnancy outcome. TM group included 21 women with a normal pregnancy outcome and 19 with subsequent complete miscarriage. The latter subgroup had significantly lower mean maternal serum (MS) sFlt-1 (83%, P<0.001) and PlGF (44%, P<0.001) compared to those with a normal pregnancy outcome. Asymptomatic control pregnant women had similar MS levels of sFlt-1 and PlGF compared to the TM patients with a normal outcome. The mean MS sFlt-1 (>10 fold) and MS PlGF (∼2 fold) levels were significantly (P<0.001) higher in control pregnant women compared to the non-pregnant group in the luteal phase of the menstrual cycle. Soluble Endoglin was not altered in the normal pregnant women compared to non pregnant women, although lower in the TM subgroup with a subsequent miscarriage (∼25%, P<0.001) compared to TM with a live birth. There was no significant difference in the mean MS HSP 70 levels between the different groups. This study shows that sFlt1 and PlGF MS levels are increased by several folds in early pregnancy and that MS sFlt-1 and MS PlGF are markedly decreased in threatened miscarriage patients who subsequently have a miscarriage suggesting these proteins are sensitive predictive markers of subsequent pregnancy loss.
Item Type:
Article
Language:
en
MeSH:
Adult; Antigens, CD; Biological Markers; Demography; Embryo Loss; Female; HSP70 Heat-Shock Proteins; Humans; Membrane Proteins; Pregnancy; Receptors, Cell Surface; Solubility; Vascular Endothelial Growth Factor Receptor-1
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorMuttukrishna, Shanthien
dc.contributor.authorSwer, Michelleen
dc.contributor.authorSuri, Sangeetaen
dc.contributor.authorJamil, Amnaen
dc.contributor.authorCalleja-Agius, Jeanen
dc.contributor.authorGangooly, Subrataen
dc.contributor.authorLudlow, Helenen
dc.contributor.authorJurkovic, Davoren
dc.contributor.authorJauniaux, Ericen
dc.date.accessioned2011-07-12T14:54:41Z-
dc.date.available2011-07-12T14:54:41Z-
dc.date.issued2011-
dc.identifier.citationSoluble Flt-1 and PlGF: new markers of early pregnancy loss? 2011, 6 (3):e18041 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21448460-
dc.identifier.doi10.1371/journal.pone.0018041-
dc.identifier.urihttp://hdl.handle.net/10147/135862-
dc.description.abstractRecent data have indicated a relationship between placental oxygen and angiogenic protein levels in the first trimester of normal pregnancies. Our objective was to investigate if maternal serum levels of angiogenic factors Soluble vascular endothelial growth factor (VEGF) receptor 1 (sFlt-1), soluble Endoglin and placental growth factor (PlGF) are altered in women with symptoms of threatened miscarriage (TM) and if they are predictive of a subsequent miscarriage. Blood samples were collected at 6-10 weeks from women presenting with TM (n = 40), from asymptomatic controls (n = 32) and from non- pregnant women in their luteal phase (n = 14). All samples were assayed for serum level of sFLT-1, PlGF, sEndoglin and HSP70 using commercial ELISAs. Samples were analysed retrospectively on the basis of pregnancy outcome. TM group included 21 women with a normal pregnancy outcome and 19 with subsequent complete miscarriage. The latter subgroup had significantly lower mean maternal serum (MS) sFlt-1 (83%, P<0.001) and PlGF (44%, P<0.001) compared to those with a normal pregnancy outcome. Asymptomatic control pregnant women had similar MS levels of sFlt-1 and PlGF compared to the TM patients with a normal outcome. The mean MS sFlt-1 (>10 fold) and MS PlGF (∼2 fold) levels were significantly (P<0.001) higher in control pregnant women compared to the non-pregnant group in the luteal phase of the menstrual cycle. Soluble Endoglin was not altered in the normal pregnant women compared to non pregnant women, although lower in the TM subgroup with a subsequent miscarriage (∼25%, P<0.001) compared to TM with a live birth. There was no significant difference in the mean MS HSP 70 levels between the different groups. This study shows that sFlt1 and PlGF MS levels are increased by several folds in early pregnancy and that MS sFlt-1 and MS PlGF are markedly decreased in threatened miscarriage patients who subsequently have a miscarriage suggesting these proteins are sensitive predictive markers of subsequent pregnancy loss.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21448460en
dc.subject.meshAdult-
dc.subject.meshAntigens, CD-
dc.subject.meshBiological Markers-
dc.subject.meshDemography-
dc.subject.meshEmbryo Loss-
dc.subject.meshFemale-
dc.subject.meshHSP70 Heat-Shock Proteins-
dc.subject.meshHumans-
dc.subject.meshMembrane Proteins-
dc.subject.meshPregnancy-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshSolubility-
dc.subject.meshVascular Endothelial Growth Factor Receptor-1-
dc.titleSoluble Flt-1 and PlGF: new markers of early pregnancy loss?en
dc.typeArticleen
dc.contributor.departmentDepartment of Obstetrics and Gynaecology, Anu Research Centre, University College Cork, Cork University Maternity Hospital, Wilton, Cork, Republic of Ireland. S.Muttukrishna@ucc.ieen
dc.identifier.journalPloS oneen
dc.description.provinceMunster-

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